Journal of Applied Genetics

, Volume 53, Issue 3, pp 343–354 | Cite as

Trichoderma biodiversity in China

  • Rui-yan Sun
  • Zhi-cheng Liu
  • Kehe Fu
  • Lili Fan
  • Jie Chen
Microbial Genetics · Original Paper


In the present study, we made further investigation into the diversity of Trichoderma in China than previous ones utilizing comprehensive approaches of morphological microscopic observation and phylogenetic analysis by detecting molecular markers. One thousand nine hundred ten Trichoderma strains were isolated from soil or other materials in China: East (Anhui, Fujian, Jiangsu, Jiangxi, Shandong, Zhejiang province and Shanghai municipality), South-West (Guizhou, Qinghai, Shanxi, Sichuan and Yunnan province, Tibet Autonomous Region and Chongqing municipality), South-East (Guangdong, Guangxi, Hainan province), and Middle China (Henan, Hubei and Hunan province). Representative isolates were verified at the species level by morphological characters and the oligonucleotide barcode program TrichoOKey v.10 and the custom BLAST server TrichoBLAST, using sequence of the ITS 1 and 2 region of the rDNA cluster and partial sequences of translation elongation factor 1-alpha(tef1-α). A total of 23 Trichoderma species were identified : T.asperellum, T.atrioviride, T.aureovriride, T.brevicompactum, T.citrioviride, T.erinaceum, T.gamsii, T.hamatum, T.harzianum (H.1ixii), T.intricatum, T.koningii (H.koningii), T.koningiopsis, T.longibranchiatum, T.pleuroticola, T.reeseii (H.jecorina), T.sinensis, T.spirale, T.stromaticum, T.tomentosum, T.velutinum, T.vermipilum, T.virens (H.virens), T.viride. Among them, 3 species: T.intricatum, T.stromaticum, T.vermipilum were first reported in China; T.harzianum (H,1ixii) was the most widely distributed species in China. This study further shows that, the highest biodiversity of Trichoderma population appeared in South-West China.


Biogeography Molecular identification Phylogenetic analysis Trichoderma 


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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Rui-yan Sun
    • 1
    • 2
  • Zhi-cheng Liu
    • 1
    • 2
  • Kehe Fu
    • 1
    • 2
  • Lili Fan
    • 1
    • 2
  • Jie Chen
    • 1
    • 2
  1. 1.Department of Resource and Environmental Science, School of Agriculture and BiologyShanghai Jiaotong UniversityShanghaiChina
  2. 2.Key Laboratory of Urban Agriculture (South), Ministry of AgricultureShanghaiChina

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