Molecular cytogenetic characterization of two high protein wheat-Thinopyrum intermedium partial amphiploids
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Fluorescence and genomic in situ hybridization (FISH and GISH) were used to establish the cytogenetic constitution of two wheat × Thinopyrum intermedium partial amphiploids H95 and 55(1-57). Both partial amphiploids are high-protein lines having resistance to leaf rust, yellow rust and powdery mildew and have in total 56 chromosomes per cell. Repetitive DNA probes (pTa71, Afa family and pSc119.2) were used to identify the individual wheat chromosomes and to reveal the distribution of these probes within the alien chromosomes. FISH detected 6B tetrasomy in H95 and a null (1D)-tetrasomy (1B) in 55(1-57). GISH was carried out using biotin labeled Th. intermedium DNA and digoxigenin labeled Pseudoroegneria spicata DNA as probes, subsequently. GISH results revealed 44 wheat chromosomes and four Thinopyrum chromosome pairs, including three S and one J chromosome pairs in line H95. Line 55(1-57), contained 42 wheat chromosomes and six Th. intermedium pairs, including two S and one JS pairs. Additionally, two identical translocated chromosome pairs with diminished affinity to the alien chromatin were detected in both amphiploids. Another two translocations were found in 55(1-57), with satellite sections from the Thinopyrum J genome.
KeywordsFISH GISH High protein content Karyotyping Wheat-Thinopyrum intermedium amphiploids
This study was supported by the AGRISAFE Programme (EU-FP7-REGPOT 2007-1, grant agreement No. 203288) and the Hungarian National Research Fund (K75381). The authors gratefully acknowledge the support of the Bilateral Cooperation (No.38) between the Bulgarian and Hungarian Academy of Sciences, and thank their Bulgarian and Hungarian collaborators for providing lab facilities and fruitful discussion during this work. Thanks are due to Barbara Harasztos for revising the manuscript linguistically.
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