A collagen-based hydrogel containing tacrolimus for bone tissue engineering
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Bone tissue engineering aims to develop bone graft structure that can heal bone defects without using autografts or allografts. The current study was conducted to promote bone regeneration using a collagen type I hydrogel containing tacrolimus. For this purpose, different amounts of tacrolimus (10 μg/ml, 100 μg/ml, and 1000 μg/ml) were loaded into the hydrogel. The resulting drug-loaded hydrogels were characterized for their porosity, swelling capacity, weight loss, drug release, blood compatibility, and cell proliferation (MTT). For functional analysis, the developed hydrogel surrounded by a film made of gelatin and polycaprolactone (PCL) was administrated in the calvarias defect of Wistar rats. The results indicated that the hydrogel has a porosity of 89.2 ± 12.5% and an appropriate swelling, drug release, and blood compatibility behavior. The in vitro results indicated that the collagen hydrogel containing 1000 μg tacrolimus was adequate in terms of cell proliferation. Finally, in vivo studies provided some evidence of the potential of the developed hydrogel for bone healing.
KeywordsTacrolimus (FK-506) Collagen type I Hydrogel Osteogenesis Bone Tissue engineering
All the authors read and approved the final manuscript.
Compliance with ethical standards
Animal experiments were approved by the ethics committee of the Shahed University (ethical code: IR.Shahed.REC.1396.005) and were carried out in accordance with the university’s guidelines.
Consent for publication
Conflict of interest
The authors declare that they have no conflict of interest.
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