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A simple coating method of PDMS microchip with PTFE for synthesis of dexamethasone-encapsulated PLGA nanoparticles

  • Zahra Mahmoodi
  • Javad MohammadnejadEmail author
  • Sajad Razavi Bazaz
  • Ali Abouei Mehrizi
  • Mohammad Adel Ghiass
  • Massoud Saidijam
  • Rassoul Dinarvand
  • Majid Ebrahimi Warkiani
  • Masoud SoleimaniEmail author
Original Article
  • 83 Downloads

Abstract

Dexamethasone is a widely used drug in medical and biological applications. Since the systematic and controllable release of this drug is of significant importance, encapsulation of this anti-inflammatory drug in poly(lactic-co-glycolic acid) (PLGA) nanoparticles can minimize uncontrolled issues. As dexamethasone-encapsulated PLGA nanoparticles are synthesized in the presence of organic solvents, poly(dimethylsiloxane) (PDMS)-based microchannels collapse due to the swelling problem. In present study, PTFE nanoparticles were used for the surface modification of the microchannels to prevent absorption and adhesion of solvents into the microchannels’ wall. The contact angle analysis of microchips after coating showed that the surface of microchannels bear the superhydrophobicity feature (140.30°) and SEM images revealed that PTFE covered the surface of PDMS, favorably. Then, the prepared microchip was tested for the synthesis of dexamethasone-loaded nanoparticles. SEM and atomic force microscopy (AFM) images of the synthesized nanoparticles represented that there was not any evidence of adhesion or absorption of nanoparticles. Furthermore, the monodispersity of nanoparticles was discernible. As AFM results revealed, the average diameters of 47, 63, and 82 nm were achieved for flow ratios of 0.01, 0.05, and 0.1, respectively. To evaluate the drug efficiency, cumulative release and encapsulation efficiency were analyzed which showed much more efficiency than the synthesized nanoparticles in the bulk mode. In addition, MTT test revealed that nanoparticles could be considered as a non-toxic material. Since the synthesis of drug-loaded nanoparticles is ubiquitous in laboratory experiments, the approach presented in this study can render more versatility in this regard.

Keywords

PTFE coating Dexamethasone Microfluidics Nanoparticle synthesis Numerical simulation 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

13346_2019_636_MOESM1_ESM.docx (311 kb)
ESM 1 (DOCX 310 kb)

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Copyright information

© Controlled Release Society 2019

Authors and Affiliations

  1. 1.Department of Life Science Engineering, Faculty of New Sciences and TechnologiesUniversity of TehranTehranIran
  2. 2.School of Biomedical EngineeringUniversity Technology of SydneySydneyAustralia
  3. 3.Tissue Engineering and Hematology Department, Faculty of Medical SciencesTarbiat Modares UniversityTehranIran
  4. 4.Research Center for Molecular MedicineHamadan University of Medical SciencesHamadanIran
  5. 5.Nanotechnology Research Centre, Novel Drug Delivery Department, Faculty of PharmacyTehran University of Medical ScienceTehranIran
  6. 6.Institute of Molecular MedicineSechenov First Moscow State UniversityMoscowRussia

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