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Formulation of topical ibuprofen solid lipid nanoparticle (SLN) gel using hot melt extrusion technique (HME) and determining its anti-inflammatory strength

  • Arvind Bagde
  • Ketan Patel
  • Shallu Kutlehria
  • Nusrat Chowdhury
  • Mandip SinghEmail author
Original Article
  • 45 Downloads

Abstract

Solid lipid nanoparticles (SLN) have been formulated using various batch processes, e.g., solvent diffusion evaporation, emulsification solvent evaporation followed by size reduction using high-pressure homogenization (HPH) or ultrasonication. However, for the manufacturing of formulations, continuous processes are always preferred over batch processes since they are more efficient and offer better quality of the end product. Hence, we developed topical SLN of ibuprofen (IBU) using hot melt extrusion (HME), prepared a gel formulation, and performed its in vitro and in vivo evaluation. Effect of different variables of HME equipment and materials used in SLN was optimized using design of experiment (DoE) approach. Stable 0.48% IBU SLN with particle size 60.2 ± 4.81 nm and entrapment efficiency 90.41 ± 3.46% were developed which further gelled using 1% carbopol 981A. Drug release study, skin deposition study, and in vivo anti-inflammatory activity studies showed 84.37 ± 4.65% drug release, 12.05 ± 0.81% drug deposition, and 40.17 ± 2.41% edema inhibition respectively in case of IBU SLN gel (IBU-SLN-G) which was significantly higher (p < 0.05) than control IBU gel (C-IBU-G) with 50.11 ± 0.57% drug release, 4.11 ± 1.10% deposition, and 20.08 ± 3.23% edema inhibition respectively. In conclusion, HME offers a single step process for manufacturing for SLN which avoids high stress particle size reduction techniques used for SLN preparation.

Keywords

Hot melt extrusion Ibuprofen Topical SLN gel Quality by design Rat paw edema 

Notes

Compliance with ethical standards

The Institutional Animal Care and Use Committee (IACUC) at Florida A&M University, FL approved all animal protocols that were observed in this study on 10th March 2016 (Protocol number: 016-03; Project number: 861412-3).

Animal studies

All institutional and national guidelines for the care and use of laboratory animals were followed.

Supplementary material

13346_2019_632_MOESM1_ESM.docx (1.3 mb)
ESM 1 (DOCX 1318 kb)

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Copyright information

© Controlled Release Society 2019

Authors and Affiliations

  • Arvind Bagde
    • 1
  • Ketan Patel
    • 1
    • 2
  • Shallu Kutlehria
    • 1
  • Nusrat Chowdhury
    • 1
  • Mandip Singh
    • 1
    Email author
  1. 1.College of Pharmacy and Pharmaceutical SciencesFlorida A&M UniversityTallahasseeUSA
  2. 2.College of Pharmacy and Health SciencesSt. John’s UniversityQueensUSA

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