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Drug Delivery and Translational Research

, Volume 9, Issue 1, pp 53–65 | Cite as

Pectin-encrusted gold nanocomposites containing phytic acid and jacalin: 1,2-dimethylhydrazine-induced colon carcinogenesis in Wistar rats, PI3K/Akt, COX-2, and serum metabolomics as potential targets

  • Malti Arya
  • Pooja Singh
  • Chandra B. Tripathi
  • Poonam Parashar
  • Mahendra Singh
  • Jovita Kanoujia
  • Anupam Guleria
  • Gaurav Kaithwas
  • Krishna P. Gupta
  • Shubhini A. Saraf
Original Article

Abstract

Phytic acid (PA) has momentous chemotherapeutic potential. Due to the chelate formation and rapid elimination, it is not popular in cancer treatment. The present work was inquested to develop a surface-modified nanoformulation of PA which prevents its speedy elimination and maximizes chemotherapeutic action. Chloroauric acid was reduced with pectin to produce pectin-gold nanoparticles (PGNPs). PGNPs were incubated with PA and jacalin for drug loading and surface modifications, respectively, to form PA-loaded jacalin-pectin-gold nanoparticles (PA-J-PGNPs). Formulation(s) were assessed for various pharmaceutical/pharmacological parameters. To validate the efficacy against colon carcinogenesis, formulation(s) were assessed in 1,2-dimethylhydrazine (DMH)-treated Wistar rats. DMH treatment distorted colonic architecture, oxidative, and hemodynamic parameters, which were favorably restored by PA-J-PGNP administration. To further confirm our deliberations, formulation(s) were also examined against DMH-altered metabolic changes and expression of markers pertaining to cellular proliferation, which was reinstated by PA-J-PGNPs. Our findings establish PA formulation(s) as a promising approach for suppression of colon carcinogenesis.

Keywords

IP6 Surface modification 1H NMR spectroscopy Hemodynamic changes Western blotting RT-PCR 

Notes

Acknowledgments

The authors are grateful to Dr. Dinesh Kumar and Mr. Amit Kumar, CBMR, SGPGIMS Campus Lucknow for their help in NMR data acquisition. The University Grants Commission, Government of India granted a senior research fellowship to MA (F1-17.1/2014-15/RGNF-2014-15-SC-UTT-70422) and PP (F1-17.1/2014-15/RGNF-2014-15-SC-UTT-63524), Department of Science and Technology (DST), Government of India, DST INSPIRE Faculty Award (Ref. No. DST/Inspire Faculty Award 2014/LSBM-120) to AG, and ICMR (Ref. no.74/1/2017-Pers (EMS)) to KPG and SAS.

Compliance with Ethical Standards

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

13346_2018_605_MOESM1_ESM.docx (260 kb)
ESM 1 (DOCX 260 kb)

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Copyright information

© Controlled Release Society 2018

Authors and Affiliations

  • Malti Arya
    • 1
  • Pooja Singh
    • 1
  • Chandra B. Tripathi
    • 1
  • Poonam Parashar
    • 1
  • Mahendra Singh
    • 1
  • Jovita Kanoujia
    • 1
  • Anupam Guleria
    • 2
  • Gaurav Kaithwas
    • 1
  • Krishna P. Gupta
    • 1
  • Shubhini A. Saraf
    • 1
  1. 1.Department of Pharmaceutical SciencesBabasaheb Bhimrao Ambedkar University (BBAU), A Central UniversityLucknowIndia
  2. 2.Centre of Biomedical Research (CBMR)Sanjay Gandhi Postgraduate Institute of Medical Sciences (SGPGIMS) CampusLucknowIndia

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