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Human herpesvirus 8 DNA detection and variant analysis in patients with multiple sclerosis

  • Sayed Mahdi Marashi
  • Aliehossadat Mostafa
  • Zabihollah Shoja
  • Ahmad Nejati
  • Shohreh Shahmahmoodi
  • Yaghoub Mollaei-Kandelous
  • Mohammad Ali Sahraian
  • Somayeh Jalilvand
Short Communication
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Abstract

Several studies reported a complex interplay between viral infections and neural cells leading to multiple sclerosis. A role for some viral infections has been proposed in MS. In this study, DNA sequences of human herpesvirus 8 (HHV-8) were searched in the peripheral blood of 54 patients with multiple sclerosis and 130 healthy subjects using nested-PCR assay to amplify ORF26 locus. Furthermore, HHV-8 positive samples were subjected to a nested-PCR to amplify K1 gene of HHV-8 followed by direct nucleotide sequencing. HHV-8 genome was detected in 18.5% (10/54) and 3% (4/130) of MS patients and controls, respectively, and the difference reached statistically significant level (P = 0.0017). Genotyping analysis revealed that genotype C was common (88.9%) in all study subjects, followed by genotype A. Our results showed higher detection of HHV-8 DNA in MS patients than control group.

Keywords

Multiple sclerosis Human herpesvirus 8 Genotypes 

Notes

Acknowledgements

This study has been funded and supported by Tehran University of Medical Sciences (TUMS), Grant Nos. 95-02-27-32301 and 93-03-27-26793.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

13337_2018_481_MOESM1_ESM.docx (19 kb)
Supplementary material 1 (DOCX 19 kb)

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Copyright information

© Indian Virological Society 2018

Authors and Affiliations

  • Sayed Mahdi Marashi
    • 1
  • Aliehossadat Mostafa
    • 1
  • Zabihollah Shoja
    • 2
  • Ahmad Nejati
    • 1
  • Shohreh Shahmahmoodi
    • 1
    • 3
  • Yaghoub Mollaei-Kandelous
    • 1
  • Mohammad Ali Sahraian
    • 4
  • Somayeh Jalilvand
    • 1
  1. 1.Department of Virology, School of Public HealthTehran University of Medical SciencesTehranIran
  2. 2.Department of VirologyPasteur Institute of IranTehranIran
  3. 3.Food Microbiology Research CenterTehran University of Medical SciencesTehranIran
  4. 4.MS Research Centre, Neuroscience InstituteTUMSTehranIran

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