Human herpesvirus 8 DNA detection and variant analysis in patients with multiple sclerosis
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Several studies reported a complex interplay between viral infections and neural cells leading to multiple sclerosis. A role for some viral infections has been proposed in MS. In this study, DNA sequences of human herpesvirus 8 (HHV-8) were searched in the peripheral blood of 54 patients with multiple sclerosis and 130 healthy subjects using nested-PCR assay to amplify ORF26 locus. Furthermore, HHV-8 positive samples were subjected to a nested-PCR to amplify K1 gene of HHV-8 followed by direct nucleotide sequencing. HHV-8 genome was detected in 18.5% (10/54) and 3% (4/130) of MS patients and controls, respectively, and the difference reached statistically significant level (P = 0.0017). Genotyping analysis revealed that genotype C was common (88.9%) in all study subjects, followed by genotype A. Our results showed higher detection of HHV-8 DNA in MS patients than control group.
KeywordsMultiple sclerosis Human herpesvirus 8 Genotypes
This study has been funded and supported by Tehran University of Medical Sciences (TUMS), Grant Nos. 95-02-27-32301 and 93-03-27-26793.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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