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VirusDisease

, Volume 30, Issue 1, pp 106–111 | Cite as

Complete nucleotide sequence of a new geminivirus isolated from Vitis vinifera in India: a symptomless host of Grapevine red blotch virus

  • Avinash Marwal
  • Rajesh Kumar
  • S. M. Paul Khurana
  • R. K. GaurEmail author
Short Communication

Abstract

Symptomless grape plants (Vitis vinifera) cultivated in Jind, Punjab, have been found to carry a Grapevine red blotch virus (GRBV). Evaluation of full length DNA sequence (3204 bp) of the virus (KU522121) has revealed similarity with mastrevirus, begomovirus, and other Grapevine red blotch viruses reported in the US and Canada. Similar to naturally growing plants, agroinfiltrated model plants with infectious clone of GRBV do not show any visible disease warning sign. To the best of our knowledge, this is the first report of a symptomless host Vitis vinifera from Indian vineyards harbouring a Grapevine geminivirus.

Keywords

Vitis vinifera Geminivirus GRBV India 

Notes

Acknowledgements

The authors are thankful to Science and Engineering Research Board – Department of Science and Technology, New Delhi, India for the financial assistance (File Nos. YSS/2015/000265 and EMR/2016/000579).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

13337_2018_477_MOESM1_ESM.png (1.1 mb)
Supplementary material 1.Vitis vinifera symptomless sample (A, B, C) collected from the grape plantation fields in Punjab. Neither the Chenopodium album (D) nor the Lycopersicon esculentum (E) plants developed any symptoms when induced with infectious construct of isolate RK-1. Even the Green House raised Grapevine plants were devoid of any observable symptoms (F). (PNG 1098 kb)
13337_2018_477_MOESM2_ESM.png (235 kb)
Supplementary material 2. Dot Blot hybridization of DNA samples extracted from the infectious construct inoculated (1 to 5) and non inoculated/healthy model plants (Abbreviated as Ca = Chenopodium album, Le = Lycopersicon esculentum and Vv = Vitis vinifera) (6 to 10). (PNG 235 kb)

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Copyright information

© Indian Virological Society 2018

Authors and Affiliations

  • Avinash Marwal
    • 2
  • Rajesh Kumar
    • 1
  • S. M. Paul Khurana
    • 3
  • R. K. Gaur
    • 1
    Email author
  1. 1.Department of Biosciences, College of Arts, Science and HumanitiesMody University of Science and TechnologyLakshmangarh, SikarIndia
  2. 2.Department of Biotechnology, Vigyan Bhawan – Block B, Main CampusMohanlal Sukhadia UniversityUdaipurIndia
  3. 3.Amity Institute of BiotechnologyAmity University HaryanaGurgaon, ManesarIndia

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