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VirusDisease

, Volume 29, Issue 3, pp 333–341 | Cite as

Full genome sequences of chicken anemia virus demonstrate mutations associated with pathogenicity in two different field isolates in Egypt

  • Sara Abdel-Mawgod
  • Amany Adel
  • Abdel-Satar Arafa
  • Hussein A. Hussein
Original Article

Abstract

Chicken anemia virus (CAV) is an important pathogen associated with immunosuppression in chicken. In this study, out of samples collected from 115 commercial poultry farms, 12 samples were CAV positive by PCR. Partial sequence and phylogenetic analysis of VP1 gene revealed that the detected viruses were clustered to genotype I (n = 3) and genotype II (n = 9). Motifs of both low (E144) and high pathogenic strains (T89, I125, Q141) were found in the three viruses of genotype I. Whereas genotype II viruses demonstrated the characteristic motifs of highly pathogenic strains (I75, T89, I125, Q141, and Q144). Three isolates representative of both genotypes (CAV/CA1, CAV/GZ1 and CAV/SK4) were selected for full genome sequencing and results revealed that the VP2 gene had two substitutions at V153 and E 175, while VP3 gene had only one substitution at C118. To evaluate virus pathogenicity, two isolates from each genotype (CAV/SK4 of genotype I and CAV/CA1 of genotype II) were intramuscularly inoculated in two groups of one-day-old specific pathogen free chicks. Eighteen days post inoculation, PCR detected CAV in 75 and 90% of chicks in group I and II; respectively. Mortalities in inoculated chicks were 5 and 20% and packed cell volume values were 0.21 and 0.19; respectively. CAV/CA1 and CAV/SK4 isolates showed pathogenic evidences at the level of genetic (Q141 and 394Q) with variable degree of virulence. In conclusion, the study reports the circulation of at least two genotypes of CAV among chicken population with mutation associated with pathogenicity.

Keywords

CAV Full genome sequence Genetic characterization Pathogenicity evidence Egypt 

Notes

Acknowledgements

The authors would like to acknowledge Naglaa Hagag, Marwa Ali, Ali Zanaty and the staff of gene analysis unit of reference laboratory of poultry production (RLQP) for the kind support.

Supplementary material

13337_2018_467_MOESM1_ESM.doc (32 kb)
Supplementary material 1 (DOC 31 kb)
13337_2018_467_MOESM2_ESM.doc (33 kb)
Supplementary material 2 (DOC 33 kb)

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Copyright information

© Indian Virological Society 2018

Authors and Affiliations

  • Sara Abdel-Mawgod
    • 1
  • Amany Adel
    • 1
  • Abdel-Satar Arafa
    • 1
  • Hussein A. Hussein
    • 2
  1. 1.Reference Laboratory for Veterinary Quality Control on Poultry ProductionAnimal Health Research InstituteDokki, GizaEgypt
  2. 2.Virology Department, Faculty of Veterinary MedicineCairo UniversityGizaEgypt

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