, Volume 28, Issue 1, pp 81–92 | Cite as

Inducible transgenic tobacco system to study the mechanisms underlying chlorosis mediated by the silencing of chloroplast heat shock protein 90

  • Sachin Ashok Bhor
  • Chika Tateda
  • Tomofumi Mochizuki
  • Ken-Taro Sekine
  • Takashi Yaeno
  • Naoto Yamaoka
  • Masamichi Nishiguchi
  • Kappei KobayashiEmail author
Original Article


Chlorosis is one of the most common symptoms of plant diseases, including those caused by viruses and viroids. Recently, a study has shown that Peach latent mosaic viroid (PLMVd) exploits host RNA silencing machinery to modulate the virus disease symptoms through the silencing of chloroplast-targeted heat shock protein 90 (Hsp90C). To understand the molecular mechanisms of chlorosis in this viroid disease, we established an experimental system suitable for studying the mechanism underlying the chlorosis induced by the RNA silencing of Hsp90C in transgenic tobacco. Hairpin RNA of the Hsp90C-specific region was expressed under the control of a dexamethasone-inducible promoter, resulted in the silencing of Hsp90C gene in 2 days and the chlorosis along with growth suppression phenotypes. Time course study suggests that a sign of chlorosis can be monitored as early as 2 days, suggesting that this experimental model is suitable for studying the molecular events taken place before and after the onset of chlorosis. During the early phase of chlorosis development, the chloroplast- and photosynthesis-related genes were downregulated. It should be noted that some pathogenesis related genes were upregulated during the early phase of chlorosis in spite of the absence of any pathogen-derived molecules in this system.


Chlorosis Chloroplast Hsp90 Inducible silencing Viroid 



We thank Kazue Obara for technical assistance. This study was supported in part by The United Graduate School of Agricultural Sciences, Ehime University, and JSPS KAKENHI grants 26292026 and 15K14664 to Kobayashi. Bhor has been supported by Rotary Yoneyama Memorial Foundation.

Supplementary material

13337_2017_361_MOESM1_ESM.docx (1.7 mb)
Supplementary material 1 (DOCX 1778 kb)


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Copyright information

© Indian Virological Society 2017

Authors and Affiliations

  1. 1.The United Graduate School of Agricultural SciencesEhime UniversityMatsuyamaJapan
  2. 2.Iwate Biotechnology Research CenterKitakamiJapan
  3. 3.Graduate School of Life and Environmental SciencesOsaka Prefecture UniversitySakaiJapan
  4. 4.Faculty of AgricultureUniversity of the RyukyusNakagamiJapan
  5. 5.Faculty of AgricultureEhime UniversityMatsuyamaJapan
  6. 6.Research Unit for CitromicsEhime UniversityMatsuyamaJapan

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