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VirusDisease

, Volume 27, Issue 3, pp 307–310 | Cite as

Molecular detection and characterization of a divergent isolate of Plantago asiatica mosaic virus in Plantago asiatica

  • Seungmo Lim
  • Davaajargal Igori
  • Fumei Zhao
  • Yun Su Do
  • In-Sook Cho
  • Gug-Seoun Choi
  • Jae Sun MoonEmail author
Short Communication

Abstract

Four samples of Plantago asiatica showing mottle or mottled yellowing symptoms were collected and three of them were confirmed to be infected with Plantago asiatica mosaic virus (PlAMV) by Illumina HiSeq 2500 paired-end RNA sequencing. Consequently, the complete genomic sequence of a Korean isolate of PlAMV (PlAMV isolate Gunwi) from P. asiatica was determined. The nucleotide sequence of PlAMV-Gunwi was most closely related to a Russian isolate of PlAMV (PlAMV-Ru) collected from P. asiatica but highly dissimilar (~23 %) to other isolates of PlAMV from other plant species. Pairwise comparisons revealed that the complete replicase protein and coat protein of PlAMV-Gunwi share 85.15–91.20 and 84.54–90.82 % amino acid sequence identities, respectively, to the corresponding proteins of other PlAMV isolates. To our knowledge, this is the first report of the natural infection of P. asiatica by PlAMV in Korea, and the divergent PlAMV isolate Gunwi expands our understanding of the epidemiology of PlAMV.

Keywords

PlAMV Divergent isolate Plantago asiatica Next-generation sequencing 

Notes

Acknowledgments

This research was carried out with the support of the Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ01007703), Rural Development Administration, Republic of Korea.

Supplementary material

13337_2016_329_MOESM1_ESM.pptx (316 kb)
Supplementary material 1 (PPTX 315 kb)
13337_2016_329_MOESM2_ESM.doc (72 kb)
Supplementary material 2 (DOC 72 kb)

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Copyright information

© Indian Virological Society 2016

Authors and Affiliations

  • Seungmo Lim
    • 1
    • 2
  • Davaajargal Igori
    • 1
    • 2
  • Fumei Zhao
    • 1
    • 2
  • Yun Su Do
    • 3
  • In-Sook Cho
    • 4
  • Gug-Seoun Choi
    • 4
  • Jae Sun Moon
    • 1
    • 2
    Email author
  1. 1.Molecular Biofarming Research CenterKorea Research Institute of Bioscience and BiotechnologyDaejeonRepublic of Korea
  2. 2.Biosystems and Bioengineering ProgramUniversity of Science and TechnologyDaejeonRepublic of Korea
  3. 3.Apple Research InstituteNational Institute of Horticultural and Herbal ScienceGunwiRepublic of Korea
  4. 4.Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal ScienceRDAWanjuRepublic of Korea

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