Advertisement

VirusDisease

, Volume 27, Issue 2, pp 154–160 | Cite as

Molecular characterization of banana bunchy top virus isolate from Sri Lanka and its genetic relationship with other isolates

  • W. A. R. T. Wickramaarachchi
  • K. S. ShankarappaEmail author
  • K. T. RangaswamyEmail author
  • M. N. Maruthi
  • R. G. A. S. Rajapakse
  • Saptarshi Ghosh
Original Article

Abstract

Bunchy top disease of banana caused by Banana bunchy top virus (BBTV, genus Babuvirus family Nanoviridae) is one of the most important constraints in production of banana in the different parts of the world. Six genomic DNA components of BBTV isolate from Kandy, Sri Lanka (BBTV-K) were amplified by polymerase chain reaction (PCR) with specific primers using total DNA extracted from banana tissues showing typical symptoms of bunchy top disease. The amplicons were of expected size of 1.0–1.1 kb, which were cloned and sequenced. Analysis of sequence data revealed the presence of six DNA components; DNA-R, DNA-U3, DNA-S, DNA-N, DNA-M and DNA-C for Sri Lanka isolate. Comparisons of sequence data of DNA components followed by the phylogenetic analysis, grouped Sri Lanka-(Kandy) isolate in the Pacific Indian Oceans (PIO) group. Sri Lanka-(Kandy) isolate of BBTV is classified a new member of PIO group based on analysis of six components of the virus.

Keywords

Banana Sri Lanka BBTV Polymerase chain reaction Sequence Phylogenetic tree 

Notes

Acknowledgments

The authors are thankful to the anonymous reviewers for their comments and suggestions.

References

  1. 1.
    Abel PP, Nelson RS, De B, Hoffmann N, Rogers SG, et al. Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene. Science. 1986;232:738–43.CrossRefPubMedGoogle Scholar
  2. 2.
    Almeida RPP, Bennett GM, Anhalt MD, Tsai CW, O’grady P. Spread of an introduced vector-borne banana virus in Hawaii. Mol Ecol. 2009;18:136–46.CrossRefPubMedGoogle Scholar
  3. 3.
    Amin I, Qazi J, Mansoor S, Ilyas M, Briddon RW. Molecular characterization of banana bunchy top virus (BBTV) from Pakistan. Virus Genes. 2008;36:191–8.CrossRefPubMedGoogle Scholar
  4. 4.
    Amin I, Ilyas M, Qazi J, Bashir R, Yadav JS, Mansoor S, Fauquet CM, Briddon RW. Identification of a major pathogenicity determinant and suppressors of RNA silencing encoded by a South Pacific isolate of banana bunchy top virus originating from Pakistan. Virus Genes. 2011;42(2):272–81.CrossRefPubMedGoogle Scholar
  5. 5.
    Anderson PK, Cunningham AA, Patel NG, Morales FJ, Epstein PR, Daszak P. Emerging infectious diseases of plants: pathogen pollution, climate change and agrotechnology drivers. Trends Ecol Evol. 2004;19:535–44.CrossRefPubMedGoogle Scholar
  6. 6.
    Banerjee AS, Roy GT, Behere SS, Roy SS, Dutta SK, Ngachan SV. Identification and characterization of a distinct banana bunchy top virus isolate of Pacific-Indian Oceans group from North-East India. Virus Res. 2014;2014(183):41–9.CrossRefGoogle Scholar
  7. 7.
    Bell KE, Dale JL, Ha CV, Vu MT, Revill PA. Characterization of Rep-encoding components associated with banana bunchy top nanovirus in Vietnam. Arch Virol. 2002;147:695–707.CrossRefPubMedGoogle Scholar
  8. 8.
    Bressan A, Watanabe S. Immunofluorescence localization of banana bunchy top virus (family Nanoviridae) within the aphid vector, Pentalonia nigronervosa, suggests a virus tropism distinct from aphid-transmitted luteovirus. Virus Res. 2011;155:520–5.CrossRefPubMedGoogle Scholar
  9. 9.
    Bucher P. Weight matrix of four eukaryotic RNA polymerase II promoter elements derived from 502 unrelated promoter sequences. J Mol Biol. 1990;212:563–78.CrossRefPubMedGoogle Scholar
  10. 10.
    Burns TM, Harding RM, Dale JL. Evidence that banana bunchy top virus has a multiple component genome. Arch Virol. 1994;137:371–80.CrossRefPubMedGoogle Scholar
  11. 11.
    Burns TM, Harding RM, Dale JL. The genome organization of banana bunchy top virus: analysis of six ssDNA components. J Gen Virol. 1995;76:1471–82.CrossRefPubMedGoogle Scholar
  12. 12.
    CABI. Banana bunchy top virus. Distribution maps of plant diseases, Map 19. 6th ed. CABI: Wallingford; 2013.Google Scholar
  13. 13.
    Chu PWG, Keese P, Qiu BS, Waterhouse PM, Gerlach WL. Putative full-length clones of the genomic DNA segments of subterranean clover stunt virus and identification of the segment coding for the viral coat protein. Virus Res. 1993;27:161–71.CrossRefPubMedGoogle Scholar
  14. 14.
    Dale JL. Banana bunchy top: an economically important tropical plant virus disease. Adv Virus Res. 1987;33:301–25.CrossRefPubMedGoogle Scholar
  15. 15.
    Elayabalan S, Subramaniam S, Selvarajan R. Banana bunchy top disease (BBTD) symptom expression in banana and strategies for transgenic resistance: a review. Emirates J Food Agric. 2015;27(1):55–74.Google Scholar
  16. 16.
    Fenoll C, Schwarz JJ, Black DM, Schneiser M, Howell SH. The intergenic region of maize streak virus contains a GC-rich element that activates rightward transcription and binds maize nuclear factors. Plant Mol Biol. 1990;15:865–77.CrossRefPubMedGoogle Scholar
  17. 17.
    Furuya N, Kawano S, Natsuaki KT. Characterization and genetic status of banana bunchy top virus isolated from Okinawa, Japan. J Gen Plant Pathol. 2005;71:68–73CrossRefGoogle Scholar
  18. 18.
    Gadd CH. Bunchy top disease of plantains (a review). Trop Agric. 1926;LXVI(1):3.Google Scholar
  19. 19.
    Hafner GJ, Harding RM, Dale JL. A DNA primer associated with banana bunchy top virus. J Gen Virol. 1997;78:479–86.CrossRefPubMedGoogle Scholar
  20. 20.
    Hafner GJ, Stafford MR, Wolter LC, Harding RM, Dale JL. Nicking and joining activity of Banana bunchy top virus replication protein in vitro. J Gen Virol. 1997;78(7):1795–9.CrossRefPubMedGoogle Scholar
  21. 21.
    Harding RM, Sadik AS, Bahielden A, Dale JL. A sensitive detection of banana bunchy top nanovirus using molecular genetic approaches. Arab J Biotech. 2000;3:103–14.Google Scholar
  22. 22.
    Hu JS, Wang M, Sether D, Xie W, Leonhardt KW. Use of polymerase chain reaction (PCR) to study transmission of banana bunchy top virus by the banana aphid (Pentalonia nigronervosa). Ann Appl Biol. 1996;128:55–64.CrossRefGoogle Scholar
  23. 23.
    Hu JM, Fu HC, Lin CH, Su HJ, Yeh HH. Reassortment and concerted evaluation in banana bunchy top virus genomes. J Virol. 2007;81(4):1746–61.CrossRefPubMedGoogle Scholar
  24. 24.
    Jones DR. Risks involved in the transfer of banana and plantain germplasm. In: Jones DR, editor. The improvement and testing of Musa: a global partnership. Proceedings of the first global conference of the international Musa testing program, FHIA, Honduras, 27–30 April 1994. Montpellier, France: INIBAP, pp. 85–98.Google Scholar
  25. 25.
    Jun TEZ, Liu ZX. Cloning and sequencing of DNA component of BBTV Hainan isolate. Chin J Agric Biotech. 2005;2(2):91–7.Google Scholar
  26. 26.
    Karan M, Harding RM, Dale JL. Evidence for two groups of banana bunchy top virus isolates. J Gen Virol. 1994;75:3541–6.CrossRefPubMedGoogle Scholar
  27. 27.
    Karan M, Harding RM, Dale JL. Association of banana bunchy top virus DNA components 2 to 6 with bunchy top disease. Mol Plant Pathol. 1977;1–16. http://www.bspp.org.uk/mppol/1997/0624karan.
  28. 28.
    Lazarowitz SG. Geminiviruses: genome structure and gene function. Crit Rev Plant Sci. 1992;11:327–49.CrossRefGoogle Scholar
  29. 29.
    Lodhi MA, Ye GN, Weeden NF, Reisch B. A simple and efficient method for DNA extraction from grapevine cultivars and Vitis species. Plant Mol Biol Rep. 1994;12:6–13.CrossRefGoogle Scholar
  30. 30.
    Lowe S, Browne M, Boudjelas S, De PM. 100 of the world’s worst invasive alien species—a selection from the global invasive species database. Published by the Invasive Species Specialist Group (ISSG) a specialist group of the Species Survival Commission (SSC) of World Conservation Union (IUCN); 2000.Google Scholar
  31. 31.
    Magee CJ. Some aspects of the bunchy top disease of banana and other Musa spp. J Proc R Soc. 1953;87:3–18.Google Scholar
  32. 32.
    Maruthi MN, Colvin J, Seal S, Gibbson G, Cooper J. Co-adaptation between cassava mosaic Gemini viruses and their local vector populations. Virus Res. 2002;86:71–85.CrossRefPubMedGoogle Scholar
  33. 33.
    Nai TY, Zhang YL, Feng TC, Wang JH, Kulye M, Yang WJ, Lin ZS, Xiong Z, Liu ZX. Cloning and sequence analysis of two banana bunchy top virus genomes in Hainan. Virus Genes. 2012;44(3):488–94.CrossRefGoogle Scholar
  34. 34.
    Niu S, Wang B, Guo X, Yu J, Wang X, Xu K, Zhai Y, Wang J, Liu Z. Identification of two RNA silencing suppressors from banana bunchy top virus. Arch Virol. 2009;154(11):1775–83.CrossRefPubMedGoogle Scholar
  35. 35.
    Register JC, Beachy RN. Resistance to TMV in transgenic plants results from interference with an early event in infection. Virology. 1988;166:524–32.CrossRefPubMedGoogle Scholar
  36. 36.
    Saunders K, Lucy A, Stanley J. DNA forms of the geminivirus African cassava mosaic virus consistent with the roiling circle mechanism of replication. Nucleic Acids Res. 1991;19(9):2325–30.CrossRefPubMedPubMedCentralGoogle Scholar
  37. 37.
    Selvarajan R, Sheeba MM, Balasubramanian V, Rajmohan R, Dhevi NL, Sasireka T. Molecular characterization of geographically different banana bunchy top virus isolates in India. Indian J Virol. 2010;21(2):110–6.CrossRefPubMedGoogle Scholar
  38. 38.
    Small W. Bunchy top disease of plantains in Ceylon. Mycol Notes (15) Trop Agric. 1928;LXXI(3):141.Google Scholar
  39. 39.
    Stain D, Martin DP, Muhire BM, Lolohea S, Halafihi M, Lepoint P, Blomme G, et al. The global distribution of Banana bunchy top virus reveals little evidence for frequent recent, human-mediated long distance dispersal events. Virus Evol. 2015;1(1):1–16.CrossRefGoogle Scholar
  40. 40.
    Tamura K, Stecher G, Daniel Peterson D, Alan Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol. 2013;30:2725–9.CrossRefPubMedPubMedCentralGoogle Scholar
  41. 41.
    Vishnoi R, Raj SK, Prasad V. Molecular characterization of an Indian isolate of banana bunchy top virus based on six genomic DNA components. Virus Genes. 2009;38:334–44.CrossRefPubMedGoogle Scholar
  42. 42.
    Vetten HJ, Chu PWG, Dale JL, Harding R, Hu J, Katul L, Kojima M, Randles JW, Sano Y, Thomas JE. In: Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA, editors. Virus taxonomy—eighth report of the international committee on taxonomy of viruses. London: Academic Press; 2004. p. 343–52.Google Scholar
  43. 43.
    Wanitchakorn R, Harding RM, Dale JL. Banana bunchy top virus DNA-3 encodes the viral coat protein. Arch Virol. 1997;142:1673–80.CrossRefPubMedGoogle Scholar
  44. 44.
    Wanitchakorn R, Hafner GJ, Harding RM, Dale JL. Functional analysis of proteins encoded by banana bunchy top virus DNA 4 to 6. J Gen Virol. 2000;81:299–306.CrossRefPubMedGoogle Scholar
  45. 45.
    Wanitchakorn R, Harding RM, Dale JL. Sequence variability in the coat protein gene of two groups of banana bunchy top isolates. Arch Virol. 2000;145:593–602.CrossRefPubMedGoogle Scholar
  46. 46.
    Wardlaw CW. The virus diseases: bunchy top. Mosaic infectious chlorosis and other virus diseases. In: Wardlaw CW, editor. Banana diseases including plantains and abaca. 2nd ed. London: Longman; 1972. p. 68–145.Google Scholar

Copyright information

© Indian Virological Society 2016

Authors and Affiliations

  1. 1.Division of Plant Pathology, Department of AgricultureHorticulture Crops Research and Development InstitutePeradeniyaSri Lanka
  2. 2.Department of Plant Pathology, K. R. C. College of Horticulture, ArabhaviUniversity of Horticultural SciencesBagalkotIndia
  3. 3.Department of Plant PathologyUniversity of Agricultural SciencesBengaluruIndia
  4. 4.Natural Resources InstituteUniversity of GreenwichKentUK

Personalised recommendations