, Volume 27, Issue 2, pp 154–160 | Cite as

Molecular characterization of banana bunchy top virus isolate from Sri Lanka and its genetic relationship with other isolates

  • W. A. R. T. Wickramaarachchi
  • K. S. ShankarappaEmail author
  • K. T. RangaswamyEmail author
  • M. N. Maruthi
  • R. G. A. S. Rajapakse
  • Saptarshi Ghosh
Original Article


Bunchy top disease of banana caused by Banana bunchy top virus (BBTV, genus Babuvirus family Nanoviridae) is one of the most important constraints in production of banana in the different parts of the world. Six genomic DNA components of BBTV isolate from Kandy, Sri Lanka (BBTV-K) were amplified by polymerase chain reaction (PCR) with specific primers using total DNA extracted from banana tissues showing typical symptoms of bunchy top disease. The amplicons were of expected size of 1.0–1.1 kb, which were cloned and sequenced. Analysis of sequence data revealed the presence of six DNA components; DNA-R, DNA-U3, DNA-S, DNA-N, DNA-M and DNA-C for Sri Lanka isolate. Comparisons of sequence data of DNA components followed by the phylogenetic analysis, grouped Sri Lanka-(Kandy) isolate in the Pacific Indian Oceans (PIO) group. Sri Lanka-(Kandy) isolate of BBTV is classified a new member of PIO group based on analysis of six components of the virus.


Banana Sri Lanka BBTV Polymerase chain reaction Sequence Phylogenetic tree 



The authors are thankful to the anonymous reviewers for their comments and suggestions.


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Copyright information

© Indian Virological Society 2016

Authors and Affiliations

  1. 1.Division of Plant Pathology, Department of AgricultureHorticulture Crops Research and Development InstitutePeradeniyaSri Lanka
  2. 2.Department of Plant Pathology, K. R. C. College of Horticulture, ArabhaviUniversity of Horticultural SciencesBagalkotIndia
  3. 3.Department of Plant PathologyUniversity of Agricultural SciencesBengaluruIndia
  4. 4.Natural Resources InstituteUniversity of GreenwichKentUK

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