VirusDisease

, Volume 25, Issue 4, pp 447–454 | Cite as

Impact of cucumber mosaic virus infection on the varietal traits of common bean cultivars in Iran

Original Article
First Online:
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Accepted:

Abstract

Cucumber mosaic virus (CMV) appears to be one of the most widespread pathogens on beans. In the present research, 49 Iranian lines and cultivars of common bean (Phaseolus vulgaris) were screened for their reaction to CMV. Plants at primary leaf stage were inoculated with CMV by rub inoculation and then they were kept in an insect-proof growth chamber at 20 °C. Three weeks postinoculation, inoculated plants were assayed based on their symptoms, growth rate, fresh and dry weights and virus titer. Results of the present study showed that a line, D81083, had moderate resistance, six lines and cultivars were found to be tolerant to the CMV and 42 lines were found to be susceptible, these plants exhibited severe symptoms and accumulated high levels of virus titer. However in the present research one moderately resistant line and six tolerant lines and cultivars were identified for use in breeding and cultivation and also for future on researches bean.

Keywords

CMV Iran Phaseolus vulgaris Resistance 
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Notes

Acknowledgments

The authors wish to thank Iranian National Plant Gene-Bank, Khomain bean research institute, Brojerd bean research institute and Plant Virology Research Center (PVRC), Shiraz, Iran for kindly providing the seeds. We thank Dr. Ali Mokhtassi-Bidgoli for his very helpful discussions on the statistics analysis.

References

  1. 1.
    Azizi A, Mozafari J, Shams-bakhsh M. Phenotypic and molecular screening of tomato germplasm for resistance to tomato yellow leaf curl virus. Iran J Biotechnol. 2008;6:1999–2206.Google Scholar
  2. 2.
    Beyzaei E, Dorri HR, Ghanbari AA, Gaffari Khaligh H, Rahmani Ghobadi A, Taheri Mazandarani M, Shahraeen N, Yousefi M Sadri. A new large seed chiti bean cultivar suitable for cultivation in temperate-cold areas of Iran. Seed Plant Improv J. 2012;28(2):335–7.Google Scholar
  3. 3.
    Bos L, Matt DZ. A strain of cucumber mosaic virus, seed- transmitted in beans. Neth J Plant Pathol. 1974;80:113–23.CrossRefGoogle Scholar
  4. 4.
    Bruckart WL, Lorbeer JW. Cucumber mosaic virus in weed hosts near commercial fields of lettuce and celery. Phytopathology. 1976;66:253–9.CrossRefGoogle Scholar
  5. 5.
    Cebolla-Cornejo J, Soler S, Gomar B, Soria MD, Nuez F. Screening capsicum germplasm for resistance to tomato spotted wilt virus (TSWV). Ann Appl Biol. 2003;143:143–52.CrossRefGoogle Scholar
  6. 6.
    Clark ME, Adams AN. Characteristics of microplate methods of enzyme linked immunosorbent assay for detection of plant viruses. J Gen Virol. 1977;34:475–83.PubMedCrossRefGoogle Scholar
  7. 7.
    Davis RF, Hampton RO. Cucumber mosaic virus isolates seed-borne in Phaseolus vulgaris: serology, host pathogen relationships and seed transmission. Phytopathology. 1986;76:999–1004.CrossRefGoogle Scholar
  8. 8.
    De Blas C, Borja MI, Saiz M, Romero J. Broad spectrum detection of cucumber mosaic virus (CMV) using the polymerase chain reaction. J Phytopathol. 1994;141:323–9.CrossRefGoogle Scholar
  9. 9.
    Dintinger J, Boissot N, Chiroleu F, Hamon S, Reynaud B. Evaluation of maize inbreds for maize strip virus and maize mosaic virus resistance: disease progress in relation to time and accumulative number of plant hoppers. Phytopathology. 2005;95:600–7.PubMedCrossRefGoogle Scholar
  10. 10.
    Dorri HR, Beizaie E, Ghanbari AA, Assadi B, Gaffari Khaligh H, Shahraeen N, Yousefi M, Dadivar M, Lak MR, Hassani Mehraban A Dorsa. A new white been cultivar, tolerant to tow spotted spider mite, for cultivation in temperate -cold areas of Iran. Seed Plant Improv J. 2012;28(1):155–7.Google Scholar
  11. 11.
    García-Arenal F, Palukaitis P. Cucumber mosaic virus. In: García-Arenal F, Palukaitis P, Mahy BWJ, Van Regenmortel MHV, editors. Encyclopedia of Virology. 3rd ed. Oxford: Academic Press; 2008. p. 614–9.CrossRefGoogle Scholar
  12. 12.
    Gildow FE, Shah DA, Sackett WM, Butzler T, Nault BA, Fleischer SJ. Transmission efficiency of cucumber mosaic virus by aphids associated with virus epidemics in snap bean. Phytopathology. 2008;98:1233–41.PubMedCrossRefGoogle Scholar
  13. 13.
    Gillaspie AGJ. New method for screening cowpea germ plasm for resistance to cucumber mosaic virus. Plant Dis. 2006;90:611–4.CrossRefGoogle Scholar
  14. 14.
    Graham PH, Vance CP. Legumes: importance and constraints to greater use. Plant Physiol. 2003;131:872–7.PubMedCentralPubMedCrossRefGoogle Scholar
  15. 15.
    Griffiths P. Breeding snap beans for cucumber mosaic virus (CMV) resistance. 101st annual international conference of the American society for horticultural science. Austin, Texas 2004: 869.Google Scholar
  16. 16.
    Hall R. Compendium of bean diseases. Minnesota: APS Press, The American Phytopathological Society; 1991.Google Scholar
  17. 17.
    Hern´andez-Verdugo S, Guevara-Gonz´alez RG, Rivera-Bustamante RF, Oyama K. Screening wild plants of Capsicum annuum for resistance to pepper huasteco virus (PHV): presence of viral DNA and differentiation among populations. Euphytica. 2001;122:31–6.CrossRefGoogle Scholar
  18. 18.
    Hong JS, Ohnishi S, Masuta C, Choi JK, Ryu KH. Infection of soybean by cucumber mosaic virus as determined by viral movement protein. Arch Virol. 2007;152:321–8.PubMedCrossRefGoogle Scholar
  19. 19.
    Hormozi-Nejad MH, Mozafari J, Rakhshandehroo F. Responses of Iranian bean cultivars to bean common mosaic virus (BCMV). 18th Iranian plant protection congress. Hamadan: University of Bu-Ali Sina; 2008. p. 24–7.Google Scholar
  20. 20.
    Jones RAC, Latham LJ. Natural resistance to cucumber mosaic virus in lupin species. Ann Appl Biol. 1996;129:523–42.CrossRefGoogle Scholar
  21. 21.
    Larsen RC, Miklas PN, Eastwell KC, Grau CR, Mondjana A. A virus disease complex devastating late season snap bean production in the Midwest. Annals reports of bean improvement cooperative, Michigan State University, Michigan; 2002. (45): 36–37.Google Scholar
  22. 22.
    Montazeri Hedesh R, Shams-Bakhsh M, Mozafari J. Evaluation of common bean lines for their reaction to tomato yellow leaf curl virus-Ir2. Crop Prot. 2011;30:163–7.CrossRefGoogle Scholar
  23. 23.
    Nault BA, Shah DA, Taylor AG. Viruses and aphids everywhere in New York snap bean fields in 2005. In Proceedings of the 2006 Empire State Fruit and Vegetable EXPO. Cornell Coop. Exten., Syracuse, NY; 2006. pp. 74–76.Google Scholar
  24. 24.
    Nault BA, Shah DA, Dillard HR, McFaul AC. Seasonal and spatial dynamics of alate aphid dispersal in snap bean fields in proximity to alfalfa and implications for virus management. Environ Entomol. 2004;33:1593–601.CrossRefGoogle Scholar
  25. 25.
    Osdaghi E, Alizadeh A, Shams-bakhsh M, Lak MR. Evaluation of common bean lines for their reaction to the common bacterial blight pathogen. Phytopathol Mediterr. 2009;48:461–8.Google Scholar
  26. 26.
    Palukaitis P, Roossinck MJ, Dietzgen RG, Francki RIB. Cucumber mosaic virus. Adv Virus Res. 1992;41:281–348.PubMedCrossRefGoogle Scholar
  27. 27.
    Rist DL, Lorbeer JW. Relationships of weed reservoirs of cucumber mosaic virus (CMV) and broad bean wilt virus (BBWV) to CMV and BBWV in commercial lettuce fields in New York. Phytopathology. 1991;81:367–71.CrossRefGoogle Scholar
  28. 28.
    Seo YS, Rojas MR, Lee JY, Lee SW, Jeon JS, Ronald P, Lucas WJ, Gilbertson RL. A viral resistance gene from common bean functions across plant families and is up-regulated in anon-virus-specific manner. Proc Natl Acad Sci USA. 2006;103:11856–61.PubMedCentralPubMedCrossRefGoogle Scholar
  29. 29.
    SAS Institute Inc., The SAS System for Windows. Release 9.0. Statistical Analysis Systems Institute, Cary, NC, USA; 2002.Google Scholar
  30. 30.
    Shah DA, Dillard HR, Mazumdar-Leighton S, Gonsalves D, Nault BA. Incidence, spatial patterns and associations among viruses in snap bean and alfalfa in New York. Plant Dis. 2006;90:203–10.CrossRefGoogle Scholar
  31. 31.
    Shahraeen N, Ghotbi T, Dezaje EA, Sahandi A. A survey of viruses affecting French bean (Phaseolus vulgaris) in Iran includes a first report of Southern bean mosaic virus and bean pod mottle virus. Plant Dis. 2005;89:1012.CrossRefGoogle Scholar
  32. 32.
    Spence NJ, Walkey DGA. Variation for pathogenicity among isolates of bean common mosaic virus in Africa and a reinterpretation of the genetic relationship between cultivars of Phaseolus vulgaris and pathotypes of BCMV. Plant Pathol. 1995;44:527–46.CrossRefGoogle Scholar
  33. 33.
    Strausbaugh CA, Myers JR, Forster RL, McClean PE. A quantitative method to screen common bean plants for resistance to bean common mosaic necrosis virus. Phytopathology. 2003;93:1430–6.PubMedCrossRefGoogle Scholar
  34. 34.
    Takahashi H, Miller J, Nozaki Y, Sukamto TM, Shah J, Hase S, Ikegami M, Ehara Y, Dinesh-Kumar SP. RCY1, an Arabidopsis thaliana RPP8/HRT family resistance gene, conferring resistance to cucumber mosaic virus requires salicylic acid, ethylene and a novel signal transduction mechanism. Plant J. 2002;32:655–67.PubMedCrossRefGoogle Scholar
  35. 35.
    Taheri Mazandarani M, Beyzaei E, Dorri HR, Ghanbari AA, Salehi P, Taheriyoon G, Mohammadkhani R, Shahraeen N, Hassani Mehraban A Shokoufa. Shokoufa a new high yielding white bean cultivar for cultivation in temperate and cold areas of Iran. Seed Plant Improv J. 2010;26(1):141–4.Google Scholar
  36. 36.
    Taheri Mazandarani M, Beyzaei E, Dorri HR, Ghanbari AA, Salehi P, Taheriyoon G, Mohammadkhani R, Shahraeen N, Hassani Mehraban A Pak. Pak, a new white bean cultivar suitable for mechanized harvesting. Seed Plant Improv J. 2010;26(1):144–6.Google Scholar
  37. 37.
    Todorović J, Vasić M, Todorović V. Dry and spring bean. Grafomark, Institute of Field and Vegetable Crops, Novi Sad, Faculty of Agriculture, Banja Luka, Serbia and BiH; 2008.Google Scholar
  38. 38.
    Tomlinson JA, Karter AL. Studies on the seed transmission of cucumber mosaic virus in chickweed (Stellaria media) in relation to the ecology of the virus. Ann Appl Biol. 1970;66:381–6.CrossRefGoogle Scholar
  39. 39.
    Wang HL, Sudarshana MR, Gilbertson RL, Lucas WJ. Analysis of cell-to-cell and long-distance movement of a bean dwarf mosaic geminivirus-green fluorescent protein reporter in host and nonhost species: identification of sites of resistance. Mol Plant Microbe Interact. 1999;12:345–55.CrossRefGoogle Scholar
  40. 40.
    Yang Y, Kim KS, Anderson EJ. Seed transmission of cucumber mosaic virus in spinach. Phytopathology. 1997;87:924–31.PubMedCrossRefGoogle Scholar
  41. 41.
    Zehnder GW, Murphy JF, Sikora EJ, Kloepper JW. Application of rhizobacteria for induced resistance. Eur J Plant Pathol. 2001;107:39–50.CrossRefGoogle Scholar

Copyright information

© Indian Virological Society 2014

Authors and Affiliations

  1. 1.Plant Pathology Department, Faculty of AgricultureTarbiat Modares UniversityTehranIran

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