, Volume 25, Issue 1, pp 114–119 | Cite as

Characterization of fowl adenoviruses associated with hydropericardium syndrome and inclusion body hepatitis in broiler chickens

  • Dinesh Mittal
  • Naresh JindalEmail author
  • Ashok Kumar Tiwari
  • Raj Singh Khokhar
Original Article


The present study was carried out for detection and molecular characterization of fowl adenoviruses (FAdVs) associated with hydropericardium syndrome or inclusion body hepatitis in commercial broiler chickens. The FAdVs were detected in liver samples from 33 commercial broiler chicken flocks by polymerase chain reaction (PCR) using hexon gene specific primers. The restriction enzyme analysis using StyI, BsiWI, MluI, AspI, BglI and ScaI enzymes of all the 33 FAdV-positive samples revealed FAdV-4 in 10 samples, FAdV-8 in five samples, FAdV-2 and FAdV-12 in two samples each, and FAdV-5 and FAdV-6 in one sample each. Twelve samples revealed the digestion pattern for more than one serotypes with FAdV-8 and FAdV-5, FAdV-8 and FAdV-7, FAdV-8 and FAdV-6, FAdV-8 and FAdV-12 being the predominant combinations indicating mixed infection. The serotypes FAdV-2 and FAdV-5 have not been detected previously in the country. The purified PCR products of FAdVs of four samples (HR 1, HR 2, HR 3 and HR 4) were cloned and sequenced. Phylogenetic analysis revealed that FAdVs of all four samples clustered in separate groups consistent with the REA pattern. In conclusion, this study reveals the presence of FAdV-2, FAdV-4, FAdV-5, FAdV-6, FAdV-7, FAdV-8 and FAdV-12 in broiler chickens affected with hydropericardium syndrome or inclusion body hepatitis in India.


Molecular characterization Broiler chickens Fowl adenovirus Hydropericardium syndrome Inclusion body hepatitis 

Supplementary material

13337_2013_183_MOESM1_ESM.jpg (4.2 mb)
Supplemental Fig. 1a Alignment of deduced amino acid sequences of hexon genevariable region of fowl adenovirus serotypes. Amino acid residuesdiffering from majority consensus are boxed. Amino acid positionsare as per Chiocca et al. [3] (JPG 4266 kb)
13337_2013_183_MOESM2_ESM.jpg (2.4 mb)
Supplemental Fig. 1b Alignment of deduced amino acid sequences of hexon genevariable region of fowl adenovirus serotypes. Amino acid residuesdiffering from majority consensus are boxed. Amino acid positionsare as per Chiocca et al. [3] (JPG 2469 kb)
13337_2013_183_MOESM3_ESM.doc (53 kb)
Supplementary material 3 (DOC 53 kb)


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Copyright information

© Indian Virological Society 2013

Authors and Affiliations

  • Dinesh Mittal
    • 3
  • Naresh Jindal
    • 1
    Email author
  • Ashok Kumar Tiwari
    • 2
  • Raj Singh Khokhar
    • 1
  1. 1.Department of Veterinary Public Health and Epidemiology, College of Veterinary SciencesLala Lajpat Rai University of Veterinary and Animal SciencesHisarIndia
  2. 2.Division of Animal BiotechnologyIndian Veterinary Research InstituteIzatnagarIndia
  3. 3.Animal Disease Research Center, College of Veterinary SciencesGuru Angad Dev Veterinary and Animal Science UniversityLudhianaIndia

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