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Molecular identification of Cucumber mosaic virus isolates of subgroup IB associated with mosaic disease of eggplant in India

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Abstract

Association of Cucumber mosaic virus (CMV) with severe mosaic disease of eggplant (Solanum melongena L.) collected from Lucknow and Kanpur, India was initially detected by host reaction and serological assay and confirmed by RT-PCR employing coat protein gene specific primers. Further, molecular identification of the virus isolates was done by cloning and sequence analysis of the complete RNA3 genome. Based on 97–99 % identities and phylogenetic relationships, the virus isolates infecting eggplant were identified as members of CMV subgroup IB.

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Acknowledgments

Authors are thankful to the Director, CSIR-National Botanical Research Institute, Lucknow for facilities to accomplish this work and author K. K. Gautam is thankful to Indian Council of Medical Research, New Delhi, India for fellowship.

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Correspondence to Shri Krishna Raj.

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Kumar, S., Gautam, K.K. & Raj, S.K. Molecular identification of Cucumber mosaic virus isolates of subgroup IB associated with mosaic disease of eggplant in India. VirusDis. 25, 129–131 (2014). https://doi.org/10.1007/s13337-013-0174-8

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  • DOI: https://doi.org/10.1007/s13337-013-0174-8

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