Molecular Characterization of Geographically Different Banana bunchy top virus Isolates in India
Banana bunchy top disease (BBTD) caused by Banana bunchy top virus (BBTV) is one of the most devastating diseases of banana and poses a serious threat for cultivars like Hill Banana (Syn: Virupakshi) and Grand Naine in India. In this study, we have cloned and sequenced the complete genome comprised of six DNA components of BBTV infecting Hill Banana grown in lower Pulney hills, Tamil Nadu State, India. The complete genome sequence of this hill banana isolate showed high degree of similarity with the corresponding sequences of BBTV isolates originating from Lucknow, Uttar Pradesh State, India, and from Fiji, Egypt, Pakistan, and Australia. In addition, sixteen coat protein (CP) and thirteen replicase genes (Rep) sequences of BBTV isolates collected from different banana growing states of India were cloned and sequenced. The replicase sequences of 13 isolates showed high degree of similarity with that of South Pacific group of BBTV isolates. However, the CP gene of BBTV isolates from Shervroy and Kodaikanal hills of Tamil Nadu showed higher amino acid sequence variability compared to other isolates. Another hill banana isolate from Meghalaya state had 23 nucleotide substitutions in the CP gene but the amino acid sequence was conserved. This is the first report of the characterization of a complete genome of BBTV occurring in the high altitudes of India. Our study revealed that the Indian BBTV isolates with distinct geographical origins belongs to the South Pacific group, except Shervroy and Kodaikanal hill isolates which neither belong to the South Pacific nor the Asian group.
KeywordsBanana bunchy top virus Babuvirus Nanoviridae South Pacific PCR Hill Banana
We thank the Department of Biotechnology, India for partial funding the project and the Director, National Research Centre for Banana, Trichy, India for facilitating this study. We also thank Dr. J. E. Thomas, Senior Principal Plant Virologist, Queensland Department of Primary Industries and Fisheries, Australia for his critical comments for improving the manuscript.
- 6.He ZF, Li HP, Xiao HG, Fan HZ. Cloning and sequencing of DNA component 3 of two BBTV strains. Nongye Shengwu Jisu Xuebao. 2001;9:145–8. (in Chinese).Google Scholar
- 7.Jones DR. Diseases of banana, abaca and enset. Wallingford: CAB International; 2000. p. 241–53.Google Scholar
- 9.Karan M, Harding RM, Dale JL. Association of Banana bunchy top virus DNA components 2 to 6 with banana bunchy top disease. Mol Plant Pathol. 1997. http://188.8.131.52/mppol/1997/0624karan/paper.html.
- 10.Kesavamoorthy RC. Radical changes in ecosystem in the Pulney hills. Proceedings of the 13th National Seminar on Banana production Technology. Coimbatore: TNAU; 1980. pp. 23–28.Google Scholar
- 11.Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: a laboratory manual. New York: Cold Spring Harbor Laboratory; 1989.Google Scholar
- 13.Singh HP. Research and development in banana plantation—national and international scenario. In: Singh HP, Mustaffa MM, editors. Banana—new innovations. New Delhi: Westville Publishing House; 2009. p. 1–21.Google Scholar
- 16.Tien E, Zhuang J, Liu ZX. Cloning and sequencing of DNA components of Banana bunchy top virus Hainan isolate. J Agric Biotechnol. 2004;12:680–4.Google Scholar
- 18.Vetten HJ, Chu PW, Dale JL, Harding RM, Hu J, Katul L, Kojima M, Randles JW, Sano Y, Thomas JE. In: Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA, editors. Virus taxonomy—eighth report of the International Committee on Taxonomy of Viruses. London: Academic Press; 2004. pp. 343–352.Google Scholar