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Indian Journal of Virology

, Volume 21, Issue 2, pp 103–109 | Cite as

A Geminivirus-Satellite Complex is Associated with Leaf Deformity of Mentha (Mint) Plants in Punjab

  • B. K. Borah
  • G. S. Cheema
  • C. K. Gill
  • I. DasguptaEmail author
Research Paper

Abstract

A widespread leaf deformity disease of mentha (mint), accompanied by whiteflies, the vectors of begomoviruses, was observed in Punjab in the last few years. The presence of begomovirus was indicated by DNA dot-blot analysis using the conserved coat protein and replication-associated protein genes of another begomovirus, Sri Lankan cassava mosaic virus (SLCMV). A DNA fragment (2.0 kb), representing a partial genomic DNA of a begomovirus, amplified from the symptomatic mentha leaves was used to design end-primers and further amplify an additional 0.9 kb fragment, representing the remaining portion of the resident viral DNA. The two sequences, assembled together (2.7 kb), showed that they represented the complete sequence of an isolate of Tomato leaf curl Karnataka virus (ToLCKV) DNA. Using universal betasatellite primers, a 1.4 kb fragment was amplified from the same sample. This cloned DNA fragment showed complete sequence identity with the previously reported Cotton leaf curl Multan betasatellite (CLCuMB). Majority of the symptomatic mentha leaf samples, collected from four districts of Punjab, showed cross-hybridization in DNA dot-blot using cloned SLCMV and CLCuMB DNA, indicating the presence of one or more begomoviruses related to SLCMV and the betasatellite, CLCuMB. The begomovirus and betasatellite could be mechanically transmitted to Nicotiana benthamiana. Whitefly transmission of the resident begomovirus could also be demonstrated on mentha. The evidence indicates the association of ToLCKV and CLCuMB, a hitherto new combination of a begomovirus and a betasatellite associated with a leaf deformity disease in mentha in Punjab.

Keywords

Mentha Begomovirus Betasatellite Whitefly 

Notes

Acknowledgments

BKB is grateful to Council of Scientific and Industrial Research, Govt. of India for Research fellowship. Authors are grateful to Dr V. G. Malathi, Indian Agricultural Research Institute, New Delhi, for providing the antisera against MYMIV.

References

  1. 1.
    Beczner L, Hamilton RI, Rochon DM. Properties of the Mentha strain of lychnis ringspot virus. Intervirology. 1992;33:49–56.PubMedGoogle Scholar
  2. 2.
    Borah BK, Johnson AMA, Sai Gopal DVR, Dasgupta I. A comparison of four DNA extraction methods for the detection of Citrus yellow mosaic badna virus from two species of citrus using PCR and dot-blot hybridization. Virus Res. 2008;151:321–4.Google Scholar
  3. 3.
    Briddon RW, Mansoor S, Bedford ID, Pinner MS, Saunders K, Stanley J, Zafar Y, Malik KA, Markham PG. Identification of DNA components required for induction of cotton leaf curl disease. Virology. 2001;285:234–43.PubMedCrossRefGoogle Scholar
  4. 4.
    Briddon RW, Bull SE, Mansoor S, Amin I, Markham PG. Universal primers for PCR-mediated amplification of DNA-β. Mol Biotechnol. 2002;20:315–8.PubMedCrossRefGoogle Scholar
  5. 5.
    Briddon RW, Stanley J. Subviral agents associated with plant single-stranded DNA viruses. Virology. 2006;344:198–210.PubMedCrossRefGoogle Scholar
  6. 6.
    Briddon RW, Brown JK, Moriones E, Stanley J, Zerbini M, Zhou X, Fauquet CM. Recommendations for the classification and nomenclature of the DNA-β satellites of begomoviruses. Arch Virol. 2008;153:763–81.PubMedCrossRefGoogle Scholar
  7. 7.
    Chakraborty S, Pandey PK, Banerjee MK, Kalloo G, Fauquet CM. Tomato leaf curl Gujarat virus, a new Begomovirus species causing a severe leaf curl disease of tomato in Varanasi, India. Phytopathology. 2003;93:1485–95.PubMedCrossRefGoogle Scholar
  8. 8.
    Chatterjee A, Ghosh SK. A new monopartite begomovirus isolated from Hibiscus cannabinus L. in India. Arch Virol. 2007;152:2113–8.PubMedCrossRefGoogle Scholar
  9. 9.
    Chowda Reddy RV, Colvin J, Muniyappa V, Seal S. Diversity and distribution of begomoviruses infecting tomato in India. Arch Virol. 2005;150:845–67.CrossRefGoogle Scholar
  10. 10.
    Clark MF, Adams AN. Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J Gen Virol. 1977;34:475–83.PubMedCrossRefGoogle Scholar
  11. 11.
    Dry IB, Krake LR, Rigden JE, Rezaian MA. A novel subviral agent associated with a geminivirus: the first report of a DNA β satellite. Proc Natl Acad Sci USA. 1999;4:7088–93.Google Scholar
  12. 12.
    Dutt N, Briddon RW, Dasgupta I. Identification of a second begomovirus, Sri Lankan cassava mosaic virus, causing cassava mosaic disease in India. Arch Virol. 2005;150:2101–8.PubMedCrossRefGoogle Scholar
  13. 13.
    Fauquet CM, Stanley J. Geminivirus classification and nomenclature: progress and problems. Ann. Appl. Biol. 2003;142:165–89.CrossRefGoogle Scholar
  14. 14.
    Fauquet CM, Briddon RW, Brown JK, Moriones E, Stanley J, Zerbini M, Zhou X. Geminivirus strain demarcation and nomenclature. Arch Virol. 2008;153:783–821.PubMedCrossRefGoogle Scholar
  15. 15.
    Gopal P, Kumar PP, Sinilal B, Jose J, Yadunandam AK, Usha R. Differential roles of C4 and β-C1 in mediating suppression of post-transcriptional gene silencing: evidence for transactivation by the C2 of Bhendi yellow vein mosaic virus, a monopartite begomovirus. Virus Res. 2007;23:8–9.Google Scholar
  16. 16.
    Harrison BD. Advances in Geminivirus research. Annu Rev Phytopathol. 1985;23:55–82.CrossRefGoogle Scholar
  17. 17.
    Hong YG, Harrison BD. Nucleotide sequences from tomato leaf curl viruses from different countries: evidence for three geographically separate branches in evolution of coat protein of whitefly transmitted geminiviruses. J Gen Virol. 1995;76:2043–9.PubMedCrossRefGoogle Scholar
  18. 18.
    Hull R. Matthews’ plant virology. San Diego: Academic Press; 2002. p. 180.Google Scholar
  19. 19.
    Jose J, Usha R. Bhendi yellow vein mosaic disease in India is caused by association of a DNA beta satellite with a begomovirus. Virology. 2003;305:310–7.PubMedCrossRefGoogle Scholar
  20. 20.
    Kirthi N, Priyadharshini CGP, Sharma P, Maiya SP, Hemalatha V, Sivaraman P, Dhawan P, Rishi N, Savithri HS. Genetic variability of begomoviruses associated with cotton leaf curl disease originating from India. Arch Virol. 2004;149:2047–57.PubMedGoogle Scholar
  21. 21.
    Kobayashi N, Horikishi T, Katsuyama H, Handa T, Takayanagi K. A simple and efficient DNA extraction method for plants, especially woody plants. Plant Tissue Culture Biotechnol. 1998;4:76–80.Google Scholar
  22. 22.
    Mansoor S, Briddon RW, Bull SE, Bedford ID, Bashir A, Hussain M, Saeed, Zafar MY, Malik KA, Fauquet C, Markham PG. Cotton leaf curl disease is associated with multiple monopartite begomoviruses supported by single DNA beta. Arch Virol. 2003;148:1969–86.PubMedCrossRefGoogle Scholar
  23. 23.
    Muniyappa V, Venkatesh HM, Ramappa HK, Kulkarni RS, Zeiden M, Tarba CY, Ghanim M, Czosnek H. Tomato leaf curl virus from Bangalore (ToLCV-Ban4): sequence comparison with Indian ToLCV isolates, detection in plants and insects, and vector relationships. Arch Virol. 2000;145:1583–98.PubMedCrossRefGoogle Scholar
  24. 24.
    Padidam M, Beachy RN, Fauquet CM. Tomato leaf curl geminivirus from India has a bipartite genome and coat protein is not essential for infectivity. J Gen Virol. 1995;76:25–35.PubMedCrossRefGoogle Scholar
  25. 25.
    Rampersad SN, Umaharan P. Detection of begomoviruses in clarified plant extracts: a comparison of standard, direct-binding, and immunocapture polymerase chain reaction techniques. Phytopathology. 2003;93:1153–7.PubMedCrossRefGoogle Scholar
  26. 26.
    Saeed SY, Zafar Y, Randles JW, Rezaian MA. A monopartite begomovirus-associated DNA beta satellite substitutes for the DNA B of a bipartite begomovirus to permit systemic infection. J Gen Virol. 2007;88:2881–9.PubMedCrossRefGoogle Scholar
  27. 27.
    Samad A, Gupta MK, Shasany AK, Ajayakumar PV and Alam M. Begomovirus related to Tomato leaf curl Pakistan virus newly reported in Mentha crops in India. New Dis. Rep. 2008;18.Google Scholar
  28. 28.
    Sambrook J, Russell DW. Molecular cloning: a laboratory manual. 3rd ed. NY: Cold Spring Harbor Press; 2001.Google Scholar
  29. 29.
    Saunders K, Stanley J. A nanovirus-like DNA component associated with yellow vein disease of Ageratum conyzoides: evidence for interfamilial recombination between plant DNA viruses. Virology. 1999;264:142–52.PubMedCrossRefGoogle Scholar
  30. 30.
    Sharma P, Rishi N, Malathi VG. Molecular cloning of coat protein gene of an Indian cotton leaf curl virus (CLCuV-HS2) isolate and its phylogenetic relationship with others members of Geminiviridae. Virus Genes. 2005;30:85–91.PubMedCrossRefGoogle Scholar
  31. 31.
    Shih SL, Tsai WS, Green SK, Hanson PM, Valand G, Kalloo G, Shrestha SK, Joshi S. Molecular characterisation of a new tomato begomovirus infecting from India. Plant Dis. 2003;87:598.CrossRefGoogle Scholar
  32. 32.
    Stanley J, Bisaro DM, Briddon RW, Brown JK, Fauquet CM, Harrison BD, Rybicki EP, Stenger DC. Geminiviridae. In: Ball LA, editor. Virus taxonomy. 8th report of the international committee on the taxonomy of viruses. London: Elsevier/Academic Press; 2005. p. 301–26.Google Scholar
  33. 33.
    Tzanetakis IE, Postman JD, Gergerich RC, Martin RR. A virus between families: nucleotide sequence and evolution of Strawberry latent ringspot virus. Virus Res. 2006;121:199–204.PubMedCrossRefGoogle Scholar
  34. 34.
    Tzanetakis IE, Postman JD, Martin RR. Mint virus X: a novel potexvirus associated with symptoms in ‘Variegata’ mint. Arch Virol. 2006;151:143–53.PubMedCrossRefGoogle Scholar
  35. 35.
    Tzanetakis IE, Postman JD, Martin RR. Identification, detection and transmission of a new vitivirus from Mentha. Arch Virol. 2007;152:2027–33.PubMedCrossRefGoogle Scholar
  36. 36.
    Zhou X, Liu Y, Robinson DJ, Harrison BD. Four DNA-A variants among Pakistan isolates of cotton leaf curl virus and their affinities to DNA-A of geminivirus isolates from okra. J Gen Virol. 1998;79:915–23.PubMedGoogle Scholar

Copyright information

© Indian Virological Society 2011

Authors and Affiliations

  • B. K. Borah
    • 1
  • G. S. Cheema
    • 2
  • C. K. Gill
    • 3
  • I. Dasgupta
    • 1
    Email author
  1. 1.South Campus, Department of Plant Molecular BiologyUniversity of DelhiNew DelhiIndia
  2. 2.Department of AgronomyPunjab Agricultural UniversityLudhianaIndia
  3. 3.Department of EntomologyPunjab Agricultural UniversityLudhianaIndia

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