A comparison of the pharmacokinetics of three different preparations of total flavones of Hippophae rhamnoides in beagle dogs after oral administration
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Pharmacokinetic properties of isorhamnetin, quercetin, and kaempferol in three different total flavones of Hippophae rhamnoides (TFH) preparations were compared after oral administration to beagle dogs by a UPLC–MS method. The pharmacokinetic results showed that C max of isorhamnetin and quercetin in TFH solid dispersion (TFH-SD) and TFH self-emulsifying (TFH-SE) preparations was significantly enhanced than that in TFH preparations (p < 0.05). The AUCs of isorhamnetin and quercetin in TFH-SD were 5.9- and 3.1-fold higher than that of TFH, while the AUCs of isorhamnetin and quercetin in TFH-SE were 3.4- and 2.4-fold higher than that of TFH. These findings suggested that the oral bioavailability of isorhamnetin and quercetin in beagle dogs can be significantly increased in TFH-SD and TFH-SE preparations compared to TFH preparations, which was helpful to explore the new forms for oral administration TFH and explain their in vivo processes.
KeywordsTotal flavones of Hippophae rhamnoides UPLC–MS Solid dispersion Self-emulsifying Pharmacokinetics Beagle dogs
This study was sponsored by the National Science Foundation of China (81303304), the Innovation Program of Shanghai Municipal Education Commission (14YZ057), the Specialized Research Fund for the Doctoral Program of Higher Education (20133107120006), and the Medical Program of Hongkou District (1301-01).
- Shi R, Qiao S, Yu DQ, Shi XW, Liu M, Jiang XJ, Wang Q, Zhang LT (2011) Simultaneous determination of five flavonoids from Scutellaria Barbata extract in rat plasma by LC-MS/MS and its application to pharmacokinetic study. J Chromatogr B Anal Technol Biomed Life Sci 879:1625–1632CrossRefGoogle Scholar
- Sun B, Sun GB, Xiao J, Chen RC, Wang X, Wu Y, Cao L, Yang ZH, Sun XB (2012) Isorhamnetin inhibits H(2)O(2)-induced activation of the intrinsic apoptotic pathway in H9c2 cardiomyocytes through scavenging reactive oxygen species and ERK inactivation. J Cell Biochem 113:473–485CrossRefPubMedGoogle Scholar
- Zhao G, Duan J, Xie Y, Lin G, Luo H, Li G, Yuan X (2013) Effects of solid dispersion and self-emulsifying formulations on the solubility, dissolution, permeability and pharmacokinetics of isorhamnetin, quercetin and kaempferol in total flavones of Hippophae rhamnoides L. Drug Dev Ind Pharm 39:1037–1045CrossRefPubMedGoogle Scholar