Advertisement

Induction of resistance to Meloidogyne incognita by DL-Beta amino butyric acid under salt stress condition

  • Zübeyir DevranEmail author
  • Ömür Baysal
Article
  • 401 Downloads

Abstract

Root-knot nematodes cause severe yield losses in crops. Though chemicals have been widely used to manage them, these chemicals have been restricted due to their hazardous environmental and health effects. In recent years, the use of bio-control agents and the induction of resistance are prominent control methods. In the present study, DL-β-Amino Butyric Acid (BABA) was tested to control Meloidogyne incognita under salt stress conditions. The results indicate that 100 mM NaCl in combination with BABA (125 μg/ml) significantly reduced gall index by 45% compared to the control. Interestingly, the lower dose of BABA combined with abiotic stress resulted in a similar efficacy to the higher dose of BABA (250 μg/ml).

Keywords

BABA Induced resistance Root-knot nematode 

Root-knot nematodes are polyphagous pests that cause severe damage to crops. Over a 100 species of root-knot nematodes have been described. Meloidogyne incognita, M. javanica and M. arenaria are the most common and important species in tropical and subtropical regions (Karssen and Moens 2006). These species are widely present in protected vegetable production areas in Turkey (Devran and Söğüt 2009, 2010; Devran and Özalp 2017).

Chemicals were once used to control root-knot nematodes (Devran and Söğüt 2010), but increasing environmental and health concerns have restricted their use (Nyczepir and Thomas 2009; Wesemael et al. 2011). Recently, alternative control measures are being considered, such as the usage of resistant plants and induction of plant resistance using different plant activators. The induction of resistance in plants can be done either through pre-inoculation with incompatible strains of pathogens or by using chemicals with no directly inhibitory effect on plant pathogenic organisms despite their remarkable inductive effects in planta (Salazar et al. 2007; Borges and Sandalio 2015). These effects may have physiological and molecular parameters.

DL-β-amino butyric acid is a chemical plant defence activator that induces plant resistance to pathogens (Cohen 2002). However, its mode of action is uncertain because the induced reactions are dependent not only on the pathosystem, but also on the mode of application (Jakab et al. 2001). The translocation and accumulation of BABA in young leaves has resulted in enhanced resistance against fungal and bacterial pathogens (Cohen 2002; Baysal et al. 2005). Previous studies have reported a novel alternative that simultaneously uses BABA and low, tolerable levels of salt stress to control bacterial spot disease caused by Pseudomonas syringae pv. tomato (Baysal et al. 2007).

The aim of this study was to induce plant resistance to Meloidogyne incognita through BABA treatment and investigate its synergistic behaviour under abiotic stress.

The isolate of M. incognita used in this study was identified in a previous study (Devran and Söğüt 2009). Commercial tomato variety, Tueza F1, (Multi Tohum, Antalya, Turkey) with four true leaves was used for experiments. Plants were grown in pots containing sandy soil in a growth chamber at 25 °C with 65% humidity during a 16:8 h photoperiod. The plants were watered and fertilized as required.

DL-β-amino butyric acid was prepared as an aqueous solution with a final concentration of 125, 250 and 500 μg/ml. Twenty-four h prior to inoculation, the adjusted BABA solutions were applied as drenching through roots (ca. 50 ml per plant). Control plants were treated with water. The plants were immersed in a 100 mM NaCl solution, and exposed to salinity stress for 10 min. Uninoculated control plants were treated with sterile distilled water for the same period of time as the treated plants. BABA application (125 μg/ml) was also done at 24 h after treatment as indicated above. Meloidogyne incognita was reared on susceptible tomato cultivar Tueza F1 (Multi Tohum, Antalya, Turkey). Plants were inoculated at the four true leaf stage with 1000 s stage juveniles of M. incognita. Plants were arranged in randomized block design with 5 replicates. All experiments were conducted in growth chamber at 25 °C. Experiments ended at 8 weeks after inoculation and each plant was harvested and root systems were carefully washed under tap water.

Egg masses in each root system were counted under the stereo binocular microscope and root galling index was determined on each root using a gall index scale of 0–10 (Barker 1985). Number of egg masses on the roots and root galling indices in each pot were analyzed with analysis of variance procedures to determine the effects of BABA and its combination with abiotic stress on M. incognita. The significance of differences among experiments were tested using Tukey test at P < 0.05 significance level with SPSS statistical program (SPSS, 16.0).

All treatments showed remarkable differences compared to the control according to the number of egg masses and galling index on roots (Table 1). The results demonstrated a synergistic effect of low dose BABA combined with abiotic stress (salt stress) on M. incognita. These findings indicated that combination of salt stress (100 mM NaCl) with four-fold less BABA (125 μg/ml) had inhibitory effect as achieved with application of 500 μg/ml BABA alone. This could significantly reduce the galling index in tomato roots. The application of NaCl (100 mM) alone resulted in a more significant effect than BABA (500 μg/ml) alone against M. incognita (Table 1).
Table 1

Effects of treatments on Meloidogyne incognita

Applications

The Number of Egg Masses

Galling Index

BABA (500 μg/ml)

94.5 ± 13.2ab

4.5 ± 0.3ab

BABA (250 μg/ml)

46.5 ± 6.8a

3.3 ± 0.3a

NaCl (100 mM)

73.5 ± 6.5ab

3.8 ± 0.3a

BABA (125 μg/ml) + NaCl (100 mM)

53.8 ± 12.8a

3.3 ± 0.3a

Control

233.0 ± 79.5b

6.0 ± 0.6b

Means in columns followed by the same letter are not significantly different (P ≤ 0.05) according to Tukey test

In previous studies, the inducer effect of BABA has been shown for fungal and bacterial pathogens on tomatoes (Lee et al. 2000; Cohen 2002; Baysal et al. 2005). BABA has also been used as a plant inducer against root-knot nematodes in tomatoes (Oka et al. 1999). Sahebani et al. (2011) investigated the effects of BABA on cucumbers infected with M. javanica and the accumulation of total phenolic compounds, hydrogen peroxide and the activity of some enzymes related to plant defence mechanisms. They showed that treating the cucumber seedlings with BABA significantly reduced the nematode galls, number of egg masses per plant and number of eggs per individual egg mass compared to the control. In the present study, BABA was tested against M. incognita on tomatoes. Our findings were in agreement with previous studies (Sahebani et al. 2011; Oka et al. 1999).

In conclusion, these results indicate that the induction of plant resistance occurred through the simultaneous application of BABA and salt stress. Salt stress alone and its combination with low doses of BABA treatments showed a synergistic effect on tomato plants against M. incognita. Salt stress has an increasingly synergistic effect on BABA’s efficacy.

Notes

Compliance with ethical standards

Conflict of interest

Authors declare that they have no conflict of interest.

References

  1. Barker KR (1985) Nematode Extraction and Bioassays. In: Barker KR, Carter CC, Sasser JN (eds) Advanced treatise on Meloidogyne: 2. Methodology. North Carolina State University Graphics, Raleigh, pp 19–35Google Scholar
  2. Baysal Ö, Gürsoy YZ, Duru A, Örnek H (2005) Induction of oxidants in tomato leaves treated with DL-ß- amino butyric acid (BABA) and infected with Clavibacter michiganensis ssp. michiganensis. Eur J Plant Pathol 112(4):361–369CrossRefGoogle Scholar
  3. Baysal Ö, Gürsoy YZ, Örnek H, Çetinel B, Teixeira da Silva JA (2007) Enhanced systemic resistance to bacterial speck disease caused by Pseudomonas syringae pv. tomato by dl-ß-aminobutyric acid under salt stress. Physiol Plantarum 129:493–506CrossRefGoogle Scholar
  4. Borges AA, Sandalio LM (2015) Induced resistance for plant defense. Front Plant Sci 6:109–110CrossRefGoogle Scholar
  5. Cohen YR (2002) DL-β-aminobutyric acid-induced resistance against plant pathogens. Plant Dıs 86:448–457Google Scholar
  6. Devran Z, Mıstanoğlu İ. Özalp T (2017) Occurrence of mixed populations of root-knot nematodes in vegetable freenhouses in Turkey, as determined by PCR screening. J Plant Dıs Protect 124:617–630Google Scholar
  7. Devran Z, Söğüt MA (2009) Distribution and identification of root-knot nematodes from Turkey. J Nematol 41(2):128–133PubMedPubMedCentralGoogle Scholar
  8. Devran Z, Söğüt MA (2010) Occurrence of virulent root-knot nematode populations on tomatoes bearing the Mi gene in protected vegetable-growing areas of Turkey. Phytoparasitica 38:245–251CrossRefGoogle Scholar
  9. Jakab G, Cottier V, Toquin V, Rigoli G, Zimmerli L, Métraux JP, Mauch-Mani B (2001) β-Aminobutyric acid-induced resistance in plants. Eur J Plant Pathol 107:29–37CrossRefGoogle Scholar
  10. Karssen G, Moens M (2006) Root-knot nematodes. In: Perry RN, Moens M (eds) Plant nematology, 2nd edn. CAB International, Wallingford, pp 59–90CrossRefGoogle Scholar
  11. Lee YK, Hong JK, Sanwald SH, Hwang BK (2000) Histological and ultrastructural comparisons of compatible, incompatible and DL-bamino-n-butyric acid-induced resistance responses of pepper stems to Phytophthora capsici. Physıol Mol Plant P 57:269–280CrossRefGoogle Scholar
  12. Nyczepir AP, Thomas SH (2009) Current and future management strategies in intensive crop production systems. In: Perry RN, Moens M, Starr JL (eds) . Root-knot nematodes. CAB International, Wallingford, pp 412–443Google Scholar
  13. Oka Y, Cohen Y, Speigel Y (1999) Local and systemic induced resistance to the root-knot nematode in tomato by DL-b-aminon-butyric acid. Phytopathology 89:1138–1143CrossRefGoogle Scholar
  14. Sahebani N, Hadavi NS, Zade FO (2011) The effects of b-amino-butyric acid on resistance of cucumber against root-knot nematode, Meloidogyne javanica. Acta Physıol Plant 33:443–450CrossRefGoogle Scholar
  15. Salazar SM, Castagnaro AP, Arias M, Chalfoun N, Tonello U, Díaz Ricci JC (2007) Induction of a defense response in strawberry mediated by an avirulent strain of Colletotrichum. Eur J Plant Pathol 117:109–122CrossRefGoogle Scholar
  16. Wesemael WML, Viaene N, Moens M (2011) Root-knot nematodes (Meloidogyne spp.) in Europe. Nematology 13:3–16CrossRefGoogle Scholar

Copyright information

© Australasian Plant Pathology Society Inc. 2018

Authors and Affiliations

  1. 1.Department of Plant Protection, Faculty of AgricultureAkdeniz UniversityAntalyaTurkey
  2. 2.Department of Molecular Biology and Genetic, Faculty of Life ScienceMuğla Sıtkı Koçman UniversityMuğlaTurkey

Personalised recommendations