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Influence of the time of fungicide application in the production of trichothecenes on field inoculated spikes from wheat grown in Argentina

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Abstract

Fusarium graminearum is considered the main causative agent of Fusarium Head Blight in wheat. The fungus produces mycotoxins of the trichothecene family that pose a significant threat to the health of animals and humans. The objective of this work was to analyze the effect of the time of fungicide application on the trichothecene production in 8 inoculated wheat varieties. The fungicide Tebuconazole was applied in two independent stages: immediately after inoculation and after 72 h of the inoculation (infective event derived from 72 h of wetting). The detection of mycotoxins was performed by gas chromatography with electron capture detector and the results were confirmed by gas chromatography coupled to a mass spectrometer. Significant levels of deoxynivalenol were found in all samples and in almost all of them 15 acetyldeoxynivalenol was found in low concentration. Less frequently, 3 acetyldeoxynivalenol was found in higher values. There were not significant differences (p > 0.05) between the time of fungicide application (immediately after inoculation in anthesis and after 72 h) in all wheat varieties studied. The expansion of the fungicide application window for effective control of trichothecenes for 72 h represents an economic advantage for the producer avoiding the use of agrochemicals by preventive applications and minimizing the risk on human health and the environment.

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Authors and Affiliations

  1. Área de Protección de Alimentos, Instituto de Tecnología de Alimentos, Instituto Nacional de Tecnología Agropecuaria, Buenos Aires, Argentina

    Natalia Pesquero, Dante Rojas & Anabel Rodriguez

  2. Laboratorio de Microbiología de Alimentos, Instituto de Micología Y Botánica. Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina

    Diana Ramirez Albuquerque & Virginia Fernandez Pinto

  3. Estacion Experimental Agropecuaria Marcos Juarez, Instituto Nacional de Tecnología Agropecuaria, Cordoba, Argentina

    Enrique Alberione

  4. Instituto de Clima Y Agua, Instituto Nacional de Tecnología Agropecuaria, Buenos Aires, Argentina

    Ricardo Moschini

Authors
  1. Natalia Pesquero
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  2. Diana Ramirez Albuquerque
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  3. Dante Rojas
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  4. Enrique Alberione
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  5. Anabel Rodriguez
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  6. Ricardo Moschini
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  7. Virginia Fernandez Pinto
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Contributions

R. Moschini, N. Pesquero, and V. Fernandez Pinto conceived the project and its components. E. Alberione performed the field trials and selected the Fusarium isolates. N. Pesquero, D. Ramirez Albuquerque and D. Rojas were responsible for the toxin analysis. R. Moschini, N. Pesquero, A. Gonzalez and V. Fernandez Pinto analyzed the data. A. Gonzalez designed the statistical analysis. R. Moschini,V. Fernandez Pinto, N. Pesquero and A. Gonzalez wrote the manuscript and all co-authors contributed to manuscript revision.

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Correspondence to Virginia Fernandez Pinto.

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All authors of the manuscript submitted to Australasian Plant Pathology, point out that we have no potential conflict of interest.

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Pesquero, N., Ramirez Albuquerque, D., Rojas, D. et al. Influence of the time of fungicide application in the production of trichothecenes on field inoculated spikes from wheat grown in Argentina. Australasian Plant Pathol. 50, 435–440 (2021). https://doi.org/10.1007/s13313-021-00792-2

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  • DOI: https://doi.org/10.1007/s13313-021-00792-2

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