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Australasian Plant Pathology

, Volume 48, Issue 6, pp 543–551 | Cite as

Xanthomonas perforans: a tomato and pepper pathogen associated with bacterial blight and dieback of Eucalyptus pellita seedlings in Indonesia

  • K. N. Bophela
  • S. N. Venter
  • M. J. Wingfield
  • A. Duran
  • M. Tarigan
  • T. A. CoutinhoEmail author
Original Paper
  • 125 Downloads

Abstract

Leaf and shoot blight, often accompanied by die-back symptoms, on Eucalyptus species, hybrids and clones have been reported from a number of countries. More than one bacterial species has been found to cause these symptoms. In this study, a leaf disease of E. pellita in Indonesia was investigated. The disease was found primarily on nursery plants and young trees that recovered within the first year of growth. Leaf samples were collected from symptomatic trees, and isolations consistently yielded a Xanthomonas sp. Sequencing of the 16S rRNA gene region and multilocus sequence analysis (MLSA) was performed on 19 of the 61 Xanthomonas isolates obtained. In the MLSA, four genes, namely, dnaK, fyuA, gyrB and rpoD, were sequenced and the isolates were identified as X. perforans. Four representative isolates, at a concentration of 106 CFU/ml, were leaf-infiltrated and spray-inoculated on to E. pellita, tomato and pepper seedlings. The type isolate of X. perforans was included in the pathogenicity trials as a positive control. All four isolates of X. perforans, inclusive of the type isolate, induced bacterial spot symptoms on tomato and pepper seedlings. They also caused water-soaked lesions on the leaves of E. pellita seedlings, characteristic of the symptoms observed in the field. This is the first report of X. perforans infecting leaves of a woody host.

Keywords

Xanthomonas perforans Eucalyptus pellita Bacterial blight Tomato Pepper 

Notes

Acknowledgements

This study was funded by the Tree Protection Co-operative Programme (TPCP), University of Pretoria and the National Research Foundation (NRF). K.N. Bophela received the NRF/DST Innovation Masters scholarship for financial support during the tenure of the research study. The authors would like to thank the staff at the DNA sequencing facility at the University of Pretoria for assistance with DNA sequencing and staff members of the Forestry and Agricultural Biotechnology Institute (FABI) for the administrative assistance during the study period.

Compliance with ethical standards

Conflict of interest

No conflict of interest exists.

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Copyright information

© Australasian Plant Pathology Society Inc. 2019

Authors and Affiliations

  1. 1.Department of Biochemistry, Genetics and MicrobiologyUniversity of PretoriaPretoriaSouth Africa
  2. 2.Forestry and Agricultural Biotechnology Institute (FABI)University of PretoriaPretoriaSouth Africa
  3. 3.AAA R&D, PT. Riau Andalan Pulp and PaperPelalawanIndonesia

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