Effective detection of Lily Symptomless Virus using the reverse transcription loop–mediated isothermal amplification method
- 11 Downloads
Lily Symptomless Virus (LSV) is prevalent in lily, causing severe economic losses in Lily production. Therefore, it is quite necessary to develop an effective method for detecting LSV. This study aimed to develop a visual Reverse Transcription Loop–Mediated Isothermal Amplification (RT-LAMP) method to detect LSV. The optimum condition was 60 min at 65 °C. The primers designed were highly specific to LSV and did not cross-react with other seven Lily viruses. The sensitivity of RT-LAMP assay for LSV was 0.0001 ng/ul. This RT-LAMP method was rapid, simple, highly specific, and sensitive and helped in the reliable visual detection of LSV in Lily plants.
KeywordsRT-LAMP Lily Carlavirus Virus detection
Thisrogram, China (Grant No.G2014070203) and agriculture research system of Shanghai, China (Grant No. 201708). These funding bodies had no role in design, collection, and analysis of t work was financially supported by Shanghai agriculture applied technology development phe study and preparation of the manuscript.
- Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, Hase T (2000) Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 28:E63Google Scholar