Australasian Plant Pathology

, Volume 48, Issue 3, pp 309–312 | Cite as

Identification of pea leaf distortion virus and Ludwigia leaf distortion betasatellite associated with yellow leaf curl disease of lima bean in Nepal

  • M. S. ShahidEmail author
  • M. Shafiq
  • B. J. Pudashini
  • G. B. Khatri-Chhetri
  • Keiko T. Natsuaki
  • Rob W. Briddon
Research Note


Lima bean (Phaseolus lunatus) plants exhibiting yellow mosaic and stunted growth symptoms, typical of begomovirus infection, were collected in Rampur city Nepal. Symptomatic plants were shown to be infected by a monopartite begomovirus in association with a betasatellite. The two begomovirus isolates associated with the disease showed 98.7% nucleotide sequence identity to previously characterized isolates of the recently identified begomovirus species Pea leaf distortion virus (PLDV) from Nepal. The associated betasatellite isolates showed highest nucleotide sequence identity at 99% to Ludwigia leaf distortion betasatellite (LuLDB) from Nepal. This is the first identification of PLDV and LuLDB infecting the grain legume Phaseolus lunatus.


Geminivirus Begomovirus Betasatellite Phaseolus lunatus 



RWB was supported by the Higher Education Commission, Govt. of Pakistan, under the “Foreign Faculty Hiring Program”.

Compliance with ethical standards

Conflict of interest

Authors declare that they have no conflict of interest.

Supplementary material

13313_2019_630_MOESM1_ESM.docx (17 kb)
Supplementary table 1 (DOCX 16 kb)
13313_2019_630_MOESM2_ESM.jpg (29 kb)
Supplementary fig. 1 Phaseolus lunatus plants exhibiting yellow mosaic and foliar crumpling symptoms (a) compared to a non-symptomatic P. lunatus plant (b). (JPG 29 kb)


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Copyright information

© Australasian Plant Pathology Society Inc. 2019

Authors and Affiliations

  1. 1.Department of Crop Sciences, College of Agricultural and Marine SciencesSultan Qaboos UniversityAl-KhodOman
  2. 2.Kisankalagi Unnat Biu-Bijan Karyakram Improved seed for farmers program (KUBK-ISFP)ButwalNepal
  3. 3.Department of Plant PathologyInstitute of Agriculture and Animal Science Tribhuvan UniversityChitwanNepal
  4. 4.Department of International Agricultural DevelopmentTokyo University of AgricultureTokyoJapan
  5. 5.Agricultural Biotechnology DivisionNational Institute for Biotechnology and Genetic EngineeringFaisalabadPakistan

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