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Molecular characterization of Mungbean yellow mosaic India virus infecting tomato (Solanum lycopersicum L.)

  • Aniruddha Kumar AgnihotriEmail author
  • Sudhakar Prasad Mishra
  • Mohammad Ansar
  • Ramesh Chandra Tripathi
  • Ravindra Singh
  • Mohammad Akram
Original Paper
  • 73 Downloads

Abstract

The severe mosaic and mild leaf curl symptoms were observed on tomato crops in the agricultural farm at Chitrakoot, Madhya Pradesh, India. Based on typical viral symptoms and high populations of whiteflies in the field, the association of begomovirus was suspected. The identification of a bipartite begomovirus associated with the disease was performed by sequence analyses of their cloned full-length genome. The complete genome sequences were submitted in GenBank under accession numbers MF683072 (DNA-A) and MF683073 (DNA-B). The sequence analyses of both genome of bipartite begomovirus revealed highest nucleotide sequence identity and close phylogenetic relationships with Mungbean yellow mosaic India virus (MYMIV) reported from India, therefore, following the ICTV guidelines, the begomovirus under study was identified as an isolate of MYMIV. Best of our knowledge, this is the first report of MYMIV infecting tomato.

Keywords

Mungbean yellow mosaic India virus Tomato Rolling circle amplification 

Notes

Acknowledgements

The authors are thankful to the Prof. N. C. Gautam, Vice-Chancellor, Mahatma Gandhi Chitrakoot Gramoday Vishwavidyalaya (MGCGV), Chitrakoot, India for providing necessary facilities and constant support.

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Copyright information

© Australasian Plant Pathology Society Inc. 2019

Authors and Affiliations

  • Aniruddha Kumar Agnihotri
    • 1
    Email author
  • Sudhakar Prasad Mishra
    • 1
  • Mohammad Ansar
    • 2
  • Ramesh Chandra Tripathi
    • 3
  • Ravindra Singh
    • 3
  • Mohammad Akram
    • 4
  1. 1.Department of Crop Sciences, Faculty of AgricultureMahatma Gandhi Chitrakoot Gramoday VishwavidyalayaSatnaIndia
  2. 2.Department of Plant PathologyBihar Agricultural UniversityBhagalpurIndia
  3. 3.Department of Biological Sciences, Faculty of Science and EnvironmentMahatma Gandhi Chitrakoot Gramoday VishwavidyalayaSatnaIndia
  4. 4.Division of Crop ProtectionICAR-Indian Institute of Pulses ResearchKanpurIndia

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