Elimination of Apple stem pitting virus from in vitro-cultured pear by an antiviral agent combined with thermotherapy
Six in vitro pear cultivars, Wonhwang, Xuehua, Conference, Stankimson, Starcrimson and Red Bastlett were infected by Apple stem pitting virus (ASPV) and were treated by combination of 25 μg/ml ribavirin and temperature 35 °C. Results showed that ribavirin could enhance the proliferation of Xuehua, Conference, Stankimson and Starcrimson and death plants were found in Xuehua and Red Bastlett and the total survival rate of the six cultivars was 93.5%. After treatment, the regenerated plants were detected by reverse transcription-polymerase chain reaction (RT-PCR). The results showed that ASPV in all regenerated plants could not be detected and the total elimination rate of the six cultivars was 92.3%. The combination showed high efficacy on elimination of ASPV.
KeywordsPear In vitro culture Apple stem pitting virus (ASPV) Chemotherapy Thermotherapy Virus elimination
This work is supported by the China Agriculture Research System CARS-29-10.
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