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Pantoea dispersa causing bulb decay of onion in Taiwan

  • Chun-Pi Chang
  • I-Hsin Sung
  • Chien-Jui Huang
Original Paper
  • 51 Downloads

Abstract

Onion (Allium cepa L.) bulbs showing symptoms similar to those caused by slippery skin disease were sampled during a market survey in Chiayi city, Taiwan during 2017–2018. Interestingly, a bacterium which phenotypically differed from Burkholderia was isolated along with the slippery skin pathogen from the rotted scales. The purified isolates were initially identified as Pantoea dispersa by the phenotypic and biochemical characterization. Further molecular identification based on the 16S rDNA sequence analysis and multilocus sequence analysis with the atpD, gyrB, and infB sequences confirmed that the isolates are P. dispersa. For pathogenicity assay, surface-sterilized onion bulbs were injected with the isolates, and P. dispersa type strain LMG 2603T was included as a reference strain. Upon artificial inoculation of onion bulbs, all P. dispersa isolates and the strain LMG 2603T induced decays of the internal fleshy scales and yellow to tan discoloration similar to those caused by onion pathogenic Pantoea spp. The same bacterium was consistently re-isolated from the artificially inoculated onion bulbs, fulfilling Koch’s postulates. Moreover, a single injection of a P. dispersa suspension at a concentration of 1 × 104 CFU/mL was sufficient to cause disease. Accordingly, this study indicates, for the first time, that P. dispersa is an onion pathogen causing bulb decay in Taiwan.

Keywords

Pantoea dispersa Allium cepa Multilocus sequence analysis 

Notes

Acknowledgements

This work was financed by the Ministry of Science and Technology (MOST, grant number 106-2311-B-415-001), Taiwan, R.O.C.

Authors’ contributions

C.J.H. conceived and designed this study. C.P.C. carried out most of the experiments. C.J.H analyzed the data and wrote the manuscript. I.H.S. helped statistical analysis and revised the manuscript critically. All authors have approved the final version of manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Research involving human participants and/or animals

The authors declare that no human participants and animals were involved in this study.

Informed consent

This manuscript is new and not being considered elsewhere. All authors have approved the submission of this manuscript.

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Copyright information

© Australasian Plant Pathology Society Inc. 2018

Authors and Affiliations

  1. 1.Department of Plant MedicineNational Chiayi UniversityChiayi CityRepublic of China

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