β– amino butyric acid mediated changes in cellular redox homeostasis confers tomato resistance to early blight
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Foliar application of β-aminobutyric acid (BABA) restricted the colonization of Alternaria solani in tomato leaves. The activities of antioxidative enzymes were studied in defence response to oxidative stress generated upon A. solani infection. At 1 dpi (days post inoculation) and 2 dpi, these defence responses are found to be prominent in BABA- primed plants as compared to untreated plants after A. solani infection. Elevated antioxidant enzyme capacity in BABA- primed plants was coupled with less lipid peroxidation. Generation of superoxide radical (O2 ._) and hydrogen peroxide (H2O2) was efficiently modulated in BABA- primed plants in response to pathogen inoculation suggesting there role in restricting fungal growth whereas, in BABA untreated plants increased accumulation of H2O2 at late stage of infection (36 and 48 h post inoculation) resulted in failed defence response. A positive correlation was observed in levels O2 ._, H2O2, and SOD activity. In addition, the activity of phenylalanine ammonia-lyase (PAL) and deposition of callose was studied as a biochemical marker of induced resistance and marker for PAMP-triggered immunity respectively. BABA- primed plants showed an elevated PAL activity over untreated infected plants at all studied time points (1, 2, 3, 5 dpi). Contradictory to previous studies, we found that in BABA- primed plants level of callose deposition is not as much of untreated plants upon A. solani infection. Overall the results indicate that BABA priming triggers changes in oxidative status suitable for the expression of resistance against A. solani.
KeywordsInduced resistance Priming Antioxidant Callose ß- aminobutyric acid Early blight
The study was supported by a grant from BCUD, Savitribai Phule Pune University, Pune, India. PR is thankful to UGC, India for the award of RGNF-UGC Fellowship.
- Alscher RG, Erturk N, Heath LS (2002) Role of superoxide dismutases (SODs) in controlling oxidative stress in plants. J Exp Biol 53:1331–1341Google Scholar
- Bacic A, Fincher GB, Stone BA (2009) Chemistry, biochemistry and biology of (1→3)-β-glucans and related polysaccharides. Academic Press, AmsterdamGoogle Scholar
- Emelianov VI, Kravchuk ZN, Poliakovskiĭ SA, Dmitriev AP (2008) Callose accumulation during treatment of tomato (Lycopersicon esculentum L.) cells with biotic elicitors. Cytol Genet 42:21–28Google Scholar
- Flors V, Leyva MO, Vicedo B, Finiti I, Real MD, García-Agustín P, Bennett AB, González-Bosch C (2007) Absence of the endo-Î²-1,4-glucanases Cel1 and Cel2 reduces susceptibility to in tomato. Plant J 52 (6):1027–1040Google Scholar
- Khan N, Vaidyanathan C (1986) A new simple spectrophotometric assay of phenylalanine ammonia-lyase. Curr Sci 55:391–393Google Scholar
- Nakano Y, Assada K (1981) Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880Google Scholar
- Romero D, Rivera ME, Cazorla FM, Codina JC, Fernández-Ortuño D, Torés JA (2008) Comparative histochemical analyses of oxidative burst and cell wall reinforcement in compatible and incompatible melon-powdery mildew (Podosphaera fusca) interactions. J Plant Physiol 165:1895–1905CrossRefPubMedGoogle Scholar
- Vanitha SC, Niranjana SR, Mortensen CN, Umesha S (2009) Bacterial wilt of tomato in Karnataka and its management by Pseudomonas fluorescens. BioControl 54 (5):685–695Google Scholar
- Xu HW, Lu Y, Tong SY, Song FB (2011) Lipid peroxidation, antioxidant enzyme activity and osmotic adjustment changes in husk leaves of maize in black soils region of Northeast China. Afr J Agric Res 6:3098–3102Google Scholar
- Zhong Y, Wang B, Yan J, Cheng L, Yao L, Xiao L, Wu T (2014) DL-β-aminobutyric acid-induced resistance in soybean against Aphis glycines Matsumura (Hemiptera: Aphididae). PLoS One 9:e85Google Scholar