Advertisement

Australasian Plant Pathology

, Volume 45, Issue 5, pp 509–512 | Cite as

Analysis of the first complete genome sequence of an Australian tomato spotted wilt virus isolate

  • Richard Moyle
  • Lara-Simone Pretorius
  • Jessica Dalton-Morgan
  • Denis Persley
  • Peer Schenk
Original Paper

Abstract

We report the first fully sequenced genome of an Australian tomato spotted wilt virus isolate (TSWV-QLD1). The tripartite genome consists of an 8914 nt L segment, a 4869 nt M segment, and a 3003 nt S segment. Phylogenetic analysis of the N protein sequence places this Australian isolate in a different clade than partially sequenced TSWV isolates from other regions of Australia.

Keywords

TSWV Tospovirus Bunyaviridae small RNA sequencing Sanger sequencing 

Notes

Compliance with ethical standards

Funding

This study was funded by the Australian Research Council (Discovery project grant number DP140103363).

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

13313_2016_435_MOESM1_ESM.xlsx (11 kb)
Table S1 (XLSX 11 kb)
13313_2016_435_MOESM2_ESM.pdf (105 kb)
Figure S1 (PDF 105 kb)
13313_2016_435_MOESM3_ESM.pdf (106 kb)
Figure S2 (PDF 106 kb)
13313_2016_435_MOESM4_ESM.pdf (106 kb)
Figure S3 (PDF 106 kb)
13313_2016_435_MOESM5_ESM.pdf (352 kb)
Figure S4 (PDF 351 kb)

References

  1. de Avila AC, de Haan P, Kormelink R, Resende Rde O, Goldbach RW, Peters D (1993) Classification of tospoviruses based on phylogeny of nucleoprotein gene sequences. J G Virol 74(Pt 2):153–159CrossRefGoogle Scholar
  2. de Haan P, Wagemakers L, Peters D, Goldbach R (1990) The S RNA segment of tomato spotted wilt virus has an ambisense character. J G Virol 71(Pt 5):1001–1007CrossRefGoogle Scholar
  3. de Haan P, Kormelink R, de Oliveira RR, van Poelwijk F, Peters D, Goldbach R (1991) Tomato spotted wilt virus L RNA encodes a putative RNA polymerase. J G Virol 72(Pt 9):2207–2216CrossRefGoogle Scholar
  4. Debreczeni DE, Lopez C, Aramburu J, Daros JA, Soler S, Galipienso L, Falk BW, Rubio L (2015) Complete sequence of three different biotypes of tomato spotted wilt virus (wild type, tomato Sw-5 resistance-breaking and pepper Tsw resistance-breaking) from Spain. Arch Virol 160(8):2117–2123CrossRefPubMedGoogle Scholar
  5. Dereeper A, Guignon V, Blanc G, Audic S, Buffet S, Chevenet F, Dufayard JF, Guindon S, Lefort V, Lescot M et al (2008) Phylogeny.Fr: robust phylogenetic analysis for the non-specialist. Nucleic Acids Res 36(Web Server issue):W465–469Google Scholar
  6. Hu ZZ, Feng ZK, Zhang ZJ, Liu YB, Tao XR (2011) Complete genome sequence of a tomato spotted wilt virus isolate from China and comparison to other TSWV isolates of different geographic origin. Arch Virol 156(10):1905–1908CrossRefPubMedGoogle Scholar
  7. Kikkert M, Verschoor A, Kormelink R, Rottier P, Goldbach R (2001) Tomato spotted wilt virus glycoproteins exhibit trafficking and localization signals that are functional in mammalian cells. J Virol 75(2):1004–1012CrossRefPubMedPubMedCentralGoogle Scholar
  8. Kormelink R, de Haan P, Meurs C, Peters D, Goldbach R (1992) The nucleotide sequence of the M RNA segment of tomato spotted wilt virus, a bunyavirus with two ambisense RNA segments. J G Virol 73(Pt 11):2795–2804CrossRefGoogle Scholar
  9. Kormelink R, Storms M, Van Lent J, Peters D, Goldbach R (1994) Expression and subcellular location of the NSM protein of tomato spotted wilt virus (TSWV), a putative viral movement protein. Virology 200(1):56–65CrossRefPubMedGoogle Scholar
  10. Lee JS, Cho WK, Kim MK, Kwak HR, Choi HS, Kim KH (2011) Complete genome sequences of three tomato spotted wilt virus isolates from tomato and pepper plants in Korea and their phylogenetic relationship to other TSWV isolates. Arch Virol 156(4):725–728CrossRefPubMedGoogle Scholar
  11. Lewandowski DJ, Adkins S (2005) The tubule-forming NSm protein from tomato spotted wilt virus complements cell-to-cell and long-distance movement of tobacco mosaic virus hybrids. Virology 342(1):26–37CrossRefPubMedGoogle Scholar
  12. Lian S, Lee JS, Cho WK, Yu J, Kim MK, Choi HS, Kim KH (2013) Phylogenetic and recombination analysis of tomato spotted wilt virus. PLoS One 8(5):e63380CrossRefPubMedPubMedCentralGoogle Scholar
  13. Lopez C, Aramburu J, Galipienso L, Soler S, Nuez F, Rubio L (2011) Evolutionary analysis of tomato Sw-5 resistance-breaking isolates of Tomato spotted wilt virus. J G Virol 92(Pt 1):210–215CrossRefGoogle Scholar
  14. Margaria P, Rosa C (2015) First complete genome sequence of a tomato spotted wilt virus isolate from the United States and its relationship to other TSWV isolates of different geographic origin. Arch Virol 160(11):2915–2920Google Scholar
  15. Margaria P, Ciuffo M, Pacifico D, Turina M (2007) Evidence that the nonstructural protein of tomato spotted wilt virus is the avirulence determinant in the interaction with resistant pepper carrying the TSW gene. Mol Plant-Microbe Interact: MPMI 20(5):547–558CrossRefPubMedGoogle Scholar
  16. Margaria P, Miozzi L, Ciuffo M, Pappu HR, Turina M (2015) The first complete genome sequences of two distinct European tomato spotted wilt virus isolates. Arch Virol 160(2):591–595CrossRefPubMedGoogle Scholar
  17. Mitter N, Koundal V, Williams S, Pappu H (2013) Differential expression of tomato spotted wilt virus-derived viral small RNAs in infected commercial and experimental host plants. PLoS One 8(10):e76276CrossRefPubMedPubMedCentralGoogle Scholar
  18. Moyle RL, Birch RG (2013) Diversity of sequences and expression patterns among alleles of a sugarcane loading stem gene. Theor Appl Genet 126(7):1775–1782CrossRefPubMedGoogle Scholar
  19. Pappu H, Pappu S, Jain R, Bertrand P, Culbreath A, McPherson R, Csinos A (1998) Sequence characteristics of natural populations of tomato spotted wilt tospovirus infecting flue-cured tobacco in Georgia. Virus Genes 17(2):169–177Google Scholar
  20. Pappu HR, Jones RA, Jain RK (2009) Global status of tospovirus epidemics in diverse cropping systems: successes achieved and challenges ahead. Virus Res 141(2):219–236CrossRefPubMedGoogle Scholar
  21. Takeda A, Sugiyama K, Nagano H, Mori M, Kaido M, Mise K, Tsuda S, Okuno T (2002) Identification of a novel RNA silencing suppressor, NSs protein of Tomato spotted wilt virus. FEBS Lett 532(1–2):75–79Google Scholar
  22. Ullman DE, German TL, Sherwood JL, Westcot DM, Cantone FA (1993) Tospovirus replication in insect vector cells - Immunocytochemical evidence that the nonstructural protein encoded by the S-Rna of tomato spotted wilt tospovirus is present in thrips vector cells. Phytopathology 83(4):456–463CrossRefGoogle Scholar
  23. Wijkamp I, Vanlent J, Kormelink R, Goldbach R, Peters D (1993) Multiplication of tomato spotted wilt virus in its insect vector, Frankliniella-Occidentalis. J Gen Virol 74:341–349CrossRefPubMedGoogle Scholar

Copyright information

© Australasian Plant Pathology Society Inc. 2016

Authors and Affiliations

  1. 1.Plant-Microbe Interactions Laboratory, School of Agriculture and Food SciencesThe University of QueenslandBrisbaneAustralia
  2. 2.Department of Agriculture and FisheriesBrisbaneAustralia

Personalised recommendations