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Australasian Plant Pathology

, Volume 44, Issue 5, pp 527–539 | Cite as

Re-classification of the causal agent of white grain disorder on wheat as three separate species of Eutiarosporella

  • Elisha Thynne
  • Megan C. McDonald
  • Margaret Evans
  • Hugh Wallwork
  • Stephen Neate
  • Peter S. Solomon
Original Paper

Abstract

In the late 1990s, a novel Botryosphaeria-like fungal pathogen was observed causing a disease on wheat in Queensland, characterised as white grain disorder (WGD). In recent years, this disease has sporadically appeared across the eastern states of Australia. In this study, internal transcribed spacer (ITS) region sequences were used to compare these fungi to other Botryosphaeriaceae spp. to show that they should be reclassified as members of the Eutiarosporella genus. Using a small population of WGD isolates, we built a three-loci maximum likelihood tree, using ITS, β-tubulin, and Elongation Factor1-α sequences to show that there are three separate Eutiarosporella spp. found in infected grain. This multigene tree, with the support of phenotypic differences between clades observed in vitro, show that that the causal agents of WGD should be delimited into three divergent species; Eutiarosporella tritici-australis, Eutiarosporella darliae, and Eutiarosporella pseudodarliae.

Keywords

Botryosphaeriaceae Botryosphaeria zeae Phylogeny Eutiarosporella White grain 

Notes

Acknowledgments

This study was supported by the Grains Research and Development Corporation (GRDC) (ANU00019). Peter Solomon is an Australian Research Council Future Fellow and Elisha Thynne is a recipient of an Australian Postgraduate Award and a GRDC Grains Industry Research Scholarship.

Supplementary material

13313_2015_367_MOESM1_ESM.docx (37 kb)
Supplementary Table 1 (DOCX 37 kb)
13313_2015_367_Fig7_ESM.jpg (554 kb)
Supplementary Fig 1

Phylogenetic analyses separate isolates of WGD fungi into three distinct clades. Tree is rooted to Botryosphaeria agaves. (JPEG 553 kb)

13313_2015_367_Fig8_ESM.jpg (1.7 mb)
Supplementary Fig 2

Phylogenetic analyses comparing isolates of WGD fungi with a range of species from the former genus of Tiarosporella spp.. Tree is rooted to Marasasiomyces karoo. (JPEG 1739 kb)

13313_2015_367_Fig9_ESM.jpg (473 kb)
Supplementary Fig 3

Alignment of EF1-α sequences of the isolates of wheat-infecting Tiarosporella spp. focusing on the region of base-deletion in B. zeae and Clade 2 B. zeae. (JPEG 473 kb)

13313_2015_367_Fig10_ESM.jpg (2.4 mb)
Supplementary Fig 4

Growth of each of the clades of wheat-infecting Tiarosporella spp. at different temperatures. (JPEG 2495 kb)

13313_2015_367_Fig11_ESM.jpg (7.5 mb)
Supplementary Fig 5

Histogram displaying the comparative conidial dimensions of Tiarosporella spp. in relation to WGD fungi. (JPEG 7644 kb)

13313_2015_367_Fig12_ESM.jpg (1.3 mb)
Supplementary Fig 6

Image of Clade 1 pigment production on liquid minimal media (with sucrose and glutamine). (JPEG 1334 kb)

13313_2015_367_Fig13_ESM.jpg (980 kb)
Supplementary Fig 7

Growth of each of the three clades on OMA, GABA-OMA,V8-OMA. (JPEG 979 kb)

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Copyright information

© Australasian Plant Pathology Society Inc. 2015

Authors and Affiliations

  • Elisha Thynne
    • 1
  • Megan C. McDonald
    • 1
  • Margaret Evans
    • 2
  • Hugh Wallwork
    • 2
  • Stephen Neate
    • 3
  • Peter S. Solomon
    • 1
  1. 1.Plant Sciences Division, Research School of BiologyThe Australian National UniversityCanberraAustralia
  2. 2.South Australian Research and Development Institute, Plant Research CentreHartley GroveUrrbraeAustralia
  3. 3.Centre for Crop Health, Institute for Agriculture and the EnvironmentUniversity of Southern QueenslandToowoombaAustralia

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