Australasian Plant Pathology

, Volume 43, Issue 6, pp 715–726 | Cite as

Proteomic analysis reveals the potential involvement of xylanase from Pyrenophora teres f. teres in net form net blotch disease of barley

Article

Abstract

The barley pathogen Pyrenophora teres f. teres (Ptt) produces proteinaceous toxins that contribute to the necrotic symptoms observed during net form net blotch (NFNB) disease. To better understand the relationship between these toxins and virulence, a proteomics approach was used to identify proteins differentially expressed in a more virulent Ptt isolate. Three proteins were identified: an endo-1,4-β-xylanase A (PttXyn11A), a cysteine hydrolase family protein (PttCHFP1) and an unknown (but conserved) secreted protein (PttSP1). PttXyn11A was homologous to a plant cell-wall degrading enzyme but also had a predicted necrosis-inducing region on the enzyme surface. PttCHFP1 showed homology to an isochorismatase, an enzyme proposed to suppress plant defence. Xylanase activity and PttXyn11A expression were greater in more virulent isolates in vitro and during the interaction respectively, suggesting that PttXyn11A plays a role in symptom development.

Keywords

Barley net blotch Net form net blotch Virulence Proteinaceous toxins Xylanase 

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Copyright information

© Australasian Plant Pathology Society Inc. 2014

Authors and Affiliations

  1. 1.School of Agriculture, Food & WineThe University of Adelaide, Waite Research Institute, PMB 1Glen OsmondAustralia

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