Australasian Plant Pathology

, Volume 43, Issue 5, pp 577–581 | Cite as

Microsatellite and mating type primers for the maize and sorghum pathogen, Exserohilum turcicum

  • M. P. Haasbroek
  • M. Craven
  • I. Barnes
  • B. G. Crampton
Article

Abstract

Northern corn leaf blight (NCLB) is a destructive foliar disease of maize that results from infection with the fungal pathogen, Exserohilum turcicum. Annual yield losses incurred from NCLB in South Africa may exceed 50 % when environmental conditions optimal for disease development prevail. In order to study the genetic diversity of E. turcicum, 13 microsatellite markers and mating type PCR primers were developed. Thirty-two primer pairs were designed from the E. turcicum genome sequence to flank microsatellite regions. A multiplex PCR assay amplifying both mating type idiomorphs was designed from the MAT1-1 and MAT1-2 gene sequences, and a protocol for multiplex PCR amplification of MAT loci was optimized. Initial screening identified 13 microsatellite regions that were polymorphic in 9 isolates of E. turcicum. To test the efficacy of the markers, 26 isolates of E. turcicum from 6 South African provinces, including 2 isolates from sorghum, were genotyped. A total of 90 alleles across 13 loci were obtained and the gene diversity ranged from 0.074 to 0.929. Cross-species amplification with E. rostratum was obtained for one SSR marker (SSR27). The MAT markers were specific to E. turcicum and could be used to differentiate isolates of E. turcicum and E. rostratum. The markers developed in this study will be useful to elucidate the population genetic structure, genetic diversity and mode of reproduction of E. turcicum on maize and sorghum.

Keywords

Fungi Mating type markers Microsatellite Multiplex PCR Northern corn leaf blight (NCLB) Setosphaeria turcica 

Supplementary material

13313_2014_289_MOESM1_ESM.pdf (19 kb)
Online resource 1(PDF 18 kb)
13313_2014_289_MOESM2_ESM.pdf (22 kb)
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Copyright information

© Australasian Plant Pathology Society Inc. 2014

Authors and Affiliations

  • M. P. Haasbroek
    • 1
  • M. Craven
    • 3
  • I. Barnes
    • 2
  • B. G. Crampton
    • 1
  1. 1.Department of Plant Science, Forestry and Agricultural Biotechnology Institute (FABI)University of PretoriaPretoriaSouth Africa
  2. 2.Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI)University of PretoriaPretoriaSouth Africa
  3. 3.Agricultural Research Council-Grain Crops InstitutePotchefstroomSouth Africa

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