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Australasian Plant Pathology

, Volume 43, Issue 2, pp 197–203 | Cite as

Use of specific differential isolates of Rhynchosporium commune to detect minor gene resistance to leaf scald in barley seedlings

  • H. Wallwork
  • M. Grcic
  • C. D. Li
  • M. J. Hayden
  • K. Chalmers
  • D. E. Mather
Article

Abstract

Major gene resistance to leaf scald caused by Rhynchosporium commune is readily detected in barley seedlings. Screening of barley lines in this manner has been used to identify and map the presence of several such major genes. Similar detection systems have not been possible for minor genes, detection of which has come from field evaluation of plants at later growth stages. Resistance contributed by such minor genes has often therefore been termed adult plant resistance (APR) and most barley lines possess some degree of such resistance to scald. The presence and genetic control of minor gene resistance has been more difficult to study due to the requirement for field screening and the partial and apparent multigenic nature of this resistance. In this paper we report the identification of isolates of R. commune and methods which enable the presence of minor genes to be detected at the seedling stage in some key Australian varieties and breeding parents. A mapping population has been used to confirm that the QTL detected in seedlings and in the field are the same.

Keywords

Adult plant resistance Resistance QTL Pathogen variation 

Notes

Acknowledgments

The Grains Research and Development Corporation for providing the research grant that funded this work. Grant Hollaway and Mark Mclean (Department of Primary Industries, Victoria) and Celeste Linde (Australian National University) for providing some isolates of R. commune. Elysia Vassos, Rebecca Fox and Greg Lott for technical assistance.

References

  1. Ali SM (1981) Barley grass as a source of pathogenic variation in Rhynchosporium secalis. Aust J Agric Res 32:21–25CrossRefGoogle Scholar
  2. Ali SM, Mayfield AH, Clare BG (1976) Pathogenicity of 203 isolates of Rhynchosporium secalis on 21 barley cultivars. Physiol Plant Pathol 9:135–143CrossRefGoogle Scholar
  3. Brown JS (1990) Pathogenic variation among isolates of Rhynchosporium secalis from barley grass growing in South Eastern Australia. Euphytica 50:81–89CrossRefGoogle Scholar
  4. Cheong J, Williams K, Wallwork H (2006) The identification of QTLs for adult plant resistance to leaf scald in barley. Aust J Agric Res 57:961–965CrossRefGoogle Scholar
  5. Close TJ, Bhat PR, Lonardi S, Wu Y, Rostocks N, Ramsay L, Druka A, Stein N, Svensson JT, Wanamaker S, Bozdag S, Roose ML, Moscou MJ, Chao S, Varshney RK, Szűcs P, Sato K, Hayes PM, Matthews DE, Kleinhofs A, Muehbauer GJ, DeYoung J, Marshall DF, Madishetty K, Fendon RD, Condamine P, Graner A, Waugh R (2009) Development and implementation of high-throughput SNP genotyping in barley. BMC Genomics 10:582PubMedCentralPubMedCrossRefGoogle Scholar
  6. Emebiri LC (2013) QTL dissection of the loss of green colour during post-anthesis grain maturation in two-rowed barley. Theor Appl Genet 126:1873–1884PubMedCrossRefGoogle Scholar
  7. Goodwin SB, Allard RW, Webster RK (1990) A nomenclature for Rhynchosporium secalis pathotypes. Phytopathology 80:1330–1336CrossRefGoogle Scholar
  8. Goodwin SB, Allard RW, Hardy SA, Webster RK (1992) Hierarchical structure of pathogenic variation among Rhynchosporium secalis populations in Idaho and Oregon. Can J Botany 70:810–817CrossRefGoogle Scholar
  9. Hanemann SGF, Cossu R, Wicker T, Röder MS (2009) Fine mapping, physical mapping and development of diagnostic markers for the Rrs2 scald resistance gene in barley. Theor Appl Genet 119:1507–1522PubMedCrossRefGoogle Scholar
  10. Jackson LF, Webster RK (1976) Race differentiation, distribution and frequency of Rhynchosporium secalis in California. Phytopathology 66:719–725CrossRefGoogle Scholar
  11. Jarosz AM, Burdon JJ (1996) Resistance to barley scald (Rhynchosporium secalis) in wild barley grass (Hordeum glaucum and Hordeum leporinum) populations in south-eastern Australia. Aust J Agric Res 47:413–425CrossRefGoogle Scholar
  12. Jensen J, Backes G, Skinnes H, Giese H (2002) Quantitative trait loci for scald resistance in barley localized by a non-interval mapping procedure. Plant Breed 121:124–128CrossRefGoogle Scholar
  13. Lander ES, Botstein D (1989) Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121:185–199PubMedCentralPubMedGoogle Scholar
  14. Manly KF, Cudmore RH, Meer JM (2001) Map Manager QTL, cross-platform software for genetic mapping. Mamm Genome 12:930–932PubMedCrossRefGoogle Scholar
  15. Mester D, Ronin Y, Hu Y, Peng I, Nevo E, Korol AB (2003) Efficient multipoint mapping: making use of dominant repulsion-phase markers. Theor Appl Genet 107:1102–1112PubMedCrossRefGoogle Scholar
  16. Newton AC, Meyer RC, Young GR et al. (2004) Disease resistance mapping in spring barley. In Proceedings of the 9th international barley genetics symposium, Brno, Czech Republic, 20–26 June 2004: 720–725.Google Scholar
  17. Schmidt D, Röder MS, Dargaz H, Wolf N, Schweizer GF, Tekauz A, Ganal MW (2001) Construction of a YAC library from barley cultivar Franka and identification of YAC-derived markers linked to the Rh2 gene conferring resistance to scald (Rhynchosporium secalis). Genome 44:1031–1040PubMedCrossRefGoogle Scholar
  18. Schweizer G, Baumer M, Daniel G, Rugel H, Röder M (1995) RFLP markers linked to scald (Rhyncosporium secalis) resistance gene Rh2 in barley. Theor Appl Genet 90:920–924PubMedCrossRefGoogle Scholar
  19. Tekauz A (1991) Pathogenic variation in Rhynchosporium secalis on barley in Canada. Can J Plant Pathol 13:298–304CrossRefGoogle Scholar
  20. Voorrips RE (2002) MapChart: software for the graphical presentation of linkage maps and QTLs. J Hered 93:77–78PubMedCrossRefGoogle Scholar
  21. Wallwork H, Grcic M (2011) The use of differential isolates of Rhynchosporium secalis to identify resistance to leaf scald in barley. Australas Plant Pathol 40:490–496CrossRefGoogle Scholar
  22. Wenzl P, Carling J, Kudrna D, Jaccoud D, Huttner E, Kleinhofs A, Killian A (2004) Diversity Arrays Technology (DArT) for whole-genome profiling of barley. PNAS 101:9915–9920Google Scholar
  23. Williams K, Donellan S, Smyl C, Scott L, Wallwork H (2003) Molecular variation in Rhynchosporium secalis isolates obtained from hotspots. Australas Plant Pathol 32:257–262CrossRefGoogle Scholar
  24. Zaffarano PL, McDonald BA, Linde CC (2011) Two new species of Rhynchosporium. Mycologia 103:195–202PubMedCrossRefGoogle Scholar
  25. Zhan J, Fitt BDL, Pinnschmidt HO, Oxley SJP, Newton AC (2008) Resistance, epidemiology and sustainable management of Rhynchosporium secalis populations on barley. Plant Pathol 57:1–14Google Scholar
  26. Zhang QF, Webster RK, Crandall BA, Jackson LF, Maroof MAS (1992) Race composition and pathogenicity associations of Rhynchosporium secalis in California. Phytopathology 82:798–803CrossRefGoogle Scholar

Copyright information

© Australasian Plant Pathology Society Inc. 2013

Authors and Affiliations

  • H. Wallwork
    • 1
    • 3
  • M. Grcic
    • 1
  • C. D. Li
    • 2
  • M. J. Hayden
    • 4
  • K. Chalmers
    • 3
  • D. E. Mather
    • 3
  1. 1.South Australian Research and Development InstituteUrrbraeAustralia
  2. 2.Department of Agriculture and FoodSouth PerthAustralia
  3. 3.School of Agriculture, Food and Wine, Waite Research InstituteUniversity of AdelaideUrrbraeAustralia
  4. 4.Department of Environment and Primary Industry, AgriBioSciences CentreLa Trobe UniversityBundooraAustralia

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