Australasian Plant Pathology

, Volume 42, Issue 4, pp 413–420 | Cite as

Pathogenicity and sporulation of Phytophthora pinifolia on Pinus radiata in Chile

  • Rodrigo Ahumada
  • Alessandro Rotella
  • Bernard Slippers
  • Michael J. Wingfield
Article

Abstract

Phytophthora pinifolia causes the needle and shoot disease of Pinus radiata known as Daño Foliar del Pino (DFP) in Chile. The first pathogenicity trials with this organism utilized mycelial plugs placed on stem wounds. These resulted in lesions in the tissue, but did not reproduce the resinous bands on the needles, which are the most characteristics symptoms of the disease under natural conditions. In this study, stem inoculations were repeated, but to complete Koch’s postulates fully, and to confirm that P. pinifolia causes the symptoms observed on naturally infected trees, zoospore/sporangial suspensions were used to inoculate pine foliage. This method produced the same symptoms observed on needles infected naturally. These results confirm that P. pinifolia is the causal agent of the Daño Foliar del Pino on P. radiata in Chile and successfully completed Koch’s postulates for the first time. Pathogenicity tests on different Pinus spp. and hybrids showed a wide range of responses to inoculation with P. pinifolia mycelial plugs, from highly susceptible to resistant. Monitoring of sporulation revealed that the sporangia commonly remain on the needles for extended periods of time and their frequency of occurrence and dispersal appear to increase during the rainy season.

Keywords

Daño Foliar del Pino Phytophthora pinifolia Pinus radiata Needle disease Sporulation monitoring 

References

  1. Ahumada R (2003) Pathogens in commercial Eucalyptus plantations in Chile, with special reference to Mycosphaerella and Botryosphaeria species. Pretoria, South Africa: University of Pretoria, M.Sc. thesisGoogle Scholar
  2. Barnes I, Crous PW, Wingfield MJ, Wingfield BD (2004) Multigene phylogenies reveal that red band needle blight of Pinus is caused by two distinct species of Dothistroma, D. septosporum and D. pini. Stud Mycol 50:551–555Google Scholar
  3. Brasier CM, Beales PA, Kirk SA, Denman S, Rose J (2005) Phytophthora kernoviae sp. nov., an invasive pathogen causing bleeding stem lesions on forest trees and foliar necroses of ornamentals in the UK. Mycol Res 109:853–859PubMedCrossRefGoogle Scholar
  4. Davidson JM, Werres S, Garbelotto M, Hansen EM, Rizzo DM (2003) Sudden oak death and associated diseases caused by Phytophthora ramorum. Plant Health Progress Online (http://www.plantmanagementnetwork.org/php)
  5. Davidson JM, Wickland AC, Patterson HA, Falk KR, Rizzo DM (2005) Transmission of Phytophthora ramorum in mixed-evergreen forest in California. Phytopathology 95:587–596PubMedCrossRefGoogle Scholar
  6. Durán A, Gryzenhout M, Slippers B, Ahumada R, Rotella A, Flores F, Wingfield BD, Wingfield MJ (2008) Phytophthora pinifolia sp. nov., associated with a serious needle disease of Pinus radiata in Chile. Plant Pathol 57:715–727CrossRefGoogle Scholar
  7. Durán A, Slippers B, Gryzenhout M, Ahumada R, Drenth A, Wingfield BD, Wingfield MJ (2009) DNA-based method for rapid identification of the pine pathogen, Phytophthora pinifolia. FEMS Microbiol Lett 298:99–104PubMedCrossRefGoogle Scholar
  8. Durán A, Gryzenhout M, Drenth A, Slippers B, Ahumada R, Wingfield BD, Wingfield MJ (2010) AFLP analysis reveals a clonal population of Phytophthora pinifolia in Chile. Fungal Biol 114:746–752PubMedCrossRefGoogle Scholar
  9. Erwin DC, Ribeiro OK (1996) Phytophthora diseases worldwide. APS Press, St. PaulGoogle Scholar
  10. Fry WE, Goodwin SB (1997) Re-emergence of potato and tomato late blight in the United States. Plant Dis 81:1349–1357CrossRefGoogle Scholar
  11. Garbelotto M, Davison JM, Ivors K, Maloney PE, Hüberli D, Koike ST, Rizzo DM (2003) Non-oak native plants are the main hosts for the sudden oak death pathogen in California. Calif Agric 57:18–23CrossRefGoogle Scholar
  12. Greslebin AG, Hansen EM (2010) Pathogenicity of Phytophthora austrocedrae on Austrocedrus chilensis and its relation with mal del ciprés in Patagonia. Plant Pathol 59:604–612CrossRefGoogle Scholar
  13. Greslebin AG, Hansen EM, Sutton W (2007) Phytophthora austrocedrae sp. nov., a new species associated with Austrocedrus chilensis mortality in Patagonia (Argentina). Mycol Res 111:308–316PubMedCrossRefGoogle Scholar
  14. Hansen EM, Hamm PB (1996) Survival of Phytophthora lateralis in infected roots of slow growth on selective media and low Port-Orford-cedar. Plant Dis 80:1075–1078CrossRefGoogle Scholar
  15. Hansen EM, Reeser P, Davidson J, Garbelotto M, Ivors K, Douhan L, Rizzo D (2003) Phytophthora nemorosa, a new species causing cankers and leaf blight of forest trees in California and Oregon, U.S.A. Mycotaxon 88:129–138Google Scholar
  16. Hansen EM, Parke JL, Sutton W (2005) Susceptibility of Oregon forest trees and shrubs to Phytophthora ramorum: a comparison of artificial inoculation and natural infection. Plant Dis 89:63–70CrossRefGoogle Scholar
  17. Heidelberger P, Welch PD (1983) Simulation run length control in the presence of an initial transient. Oper Res 31:1109–1144CrossRefGoogle Scholar
  18. Hüberli D, Tommerup IC, Colquhoun IJ, Hardy G (2002) Evaluation of resistance to Phytophthora cinnamomi in seed-grown trees and clonal lines of Eucalyptus marginata inoculated in lateral branches and roots. Plant Pathol 51(4):435–442CrossRefGoogle Scholar
  19. Lanfranco D (2000) Manejo de plagas forestales en Chile: análisis de casos en Pinus radiata. Sér Téc IPEF 13:41–48Google Scholar
  20. Lunn DJ, Thomas A, Best N, Spiegelhalter D (2000) WinBUGS: a Bayesian modeling framework: concepts, structure, and extensibility. Stat Comput 10:325–337CrossRefGoogle Scholar
  21. Oh E, Hansen EM, Sniezko RA (2006) Port-Orford-cedar resistant to Phytophthora lateralis. For Pathol 36:385–394CrossRefGoogle Scholar
  22. Rizzo DM, Garbelotto M, Davidson JM, Slaughter GW, Koike T (2002) Phytophthora ramorum as the cause of extensive mortality of Quercus sp. and Lithocarpus densiflorus in California. Plant Dis 86:205–214CrossRefGoogle Scholar
  23. Rizzo DM, Garbelotto M, Hansen EM (2005) Phytophthora ramorum: integrative research and management of an emerging pathogen in California and Oregon forests. Annu Rev Phytopathol 43:309–345PubMedCrossRefGoogle Scholar
  24. Stat Soft Inc (2004) STATISTICA (data analysis software system), version 6. Statsoft, Tulsa, OK, USAGoogle Scholar
  25. Tooley PW, Browning M (2009) Susceptibility to Phytophthora ramorum and inoculum production potential of some common Eastern forest understory plant species. Plant Dis 93:249–256CrossRefGoogle Scholar
  26. Toro J, Gessel SP (1999) Radiata pine plantations in Chile. New Forest 18:33–44CrossRefGoogle Scholar

Copyright information

© Australasian Plant Pathology Society Inc. 2013

Authors and Affiliations

  • Rodrigo Ahumada
    • 1
    • 2
  • Alessandro Rotella
    • 1
  • Bernard Slippers
    • 3
  • Michael J. Wingfield
    • 2
  1. 1.Bioforest S.A.ConcepciónChile
  2. 2.Department of Microbiology and Plant Pathology, Forestry and Agricultural Biotechnology Institute (FABI)University of PretoriaPretoriaSouth Africa
  3. 3.Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI)University of PretoriaPretoriaSouth Africa

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