Australasian Plant Pathology

, Volume 42, Issue 4, pp 449–459 | Cite as

Infection pathway of Botrytis cinerea in capsicum fruit (Capsicum annuum L.)

  • Thong D. Le
  • Glenn McDonald
  • Eileen S. Scott
  • Amanda J. Able


Botrytis cinerea, which causes grey mould, infects fruit of a number of horticultural crops during their development but then remains latent until the ripening process when the disease manifests. However, how B. cinerea grows in capsicum fruit after harvest has not been fully characterised. The present research has examined the growth of B. cinerea in fruit of two cultivars of capsicum (cv. Aries and cv. Papri Queen) that were inoculated either before or after harvest. Three concentrations of conidial suspensions (104, 105 and 106 conidia mL−1) were used to inoculate flowers at three preharvest stages – anthesis, 3 days after anthesis (DAA) and 6 DAA, and fruit at three postharvest ripening stages – deep green (DG), breaker red (BR) and red (R). Inoculation with water served as a control. Rot development was then monitored daily during postharvest storage at 10 °C by measuring the length and width of lesions. Cv. Aries was more susceptible to B. cinerea than cv. Papri Queen regardless of whether inoculation occurred preharvest or postharvest. Flowers often died when inoculated at anthesis. Regardless of cultivar, as inoculum concentration increased the number of flowers that died also increased. However, disease development on fruit was not affected by inoculum concentration or the timing of inoculation before harvest. When fruit were inoculated after harvest, grey mould developed most rapidly in BR fruit of cv. Papri Queen and in R fruit of cv. Aries. The understanding of infection of B. cinerea revealed by this research and its implications for disease management are discussed.


Preharvest and postharvest inoculation Flowering Fruit ripening Latent infection Grey mould 



The authors thank Monsanto Seeds and Fairbanks Seeds for supplying capsicum seeds. The first author is grateful to the Agricultural Science and Technology Project of Vietnam for awarding a PhD scholarship. We thank Sue Pederick (South Australian Research and Development Institute) for sharing her techniques for long-term storage of B. cinerea and Dr Olena Kravchuk (the University of Adelaide) for her advice about multifactorial analysis.


  1. Adrian M, Jeandet P, Veneau J, Weston LA, Bessis R (1997) Biological activity of resveratrol, a stilbenic compound from grapevines, against Botrytis cinerea, the causal agent for gray mold. J Chem Ecol 23:1689–1702CrossRefGoogle Scholar
  2. Babalar M, Asghari M, Talaei A, Khosroshahi A (2007) Effect of pre-and postharvest salicylic acid treatment on ethylene production, fungal decay and overall quality of Selva strawberry fruit. Food Chem 105:449–453CrossRefGoogle Scholar
  3. Bais AJ, Murphy PJ, Dry IB (2000) The molecular regulation of stilbene phytoalexin biosynthesis in Vitis vinifera during grape berry development. Funct Plant Biol 27:723CrossRefGoogle Scholar
  4. Baker KF (1957) The U.C. system for producing healthy container-grown plants: University of California, Agricultural Experimental Station Manual 23Google Scholar
  5. Barkai-Golan R, Lavy-Meir G (1989) Effects of ethylene on the susceptibility to Botrytis cinerea infection of different tomato genotypes. Ann Appl Biol 114:391–396CrossRefGoogle Scholar
  6. Bristow P, McNicol R, Williamson B (1986) Infection of strawberry flowers by Botrytis cinerea and its relevance to grey mould development. Ann Appl Biol 109:545–554CrossRefGoogle Scholar
  7. Cantu D, Blanco-Ulate B, Yang L, Labavitch JM, Bennett AB, Powell ALT (2009) Ripening-regulated susceptibility of tomato fruit to Botrytis cinerea requires NOR but not RIN or ethylene. Plant Physiol 150:1434–1449PubMedCrossRefGoogle Scholar
  8. Conforti F, Statti GA, Menichini F (2007) Chemical and biological variability of hot pepper fruits (Capsicum annuum var. acuminatum L.) in relation to maturity stage. Food Chem 102:1096–1104CrossRefGoogle Scholar
  9. Cota I, Troncoso-Rojas R, Sotelo-Mundo R, Sánchez-Estrada A, Tiznado-Hernández M (2007) Chitinase and β-1, 3-glucanase enzymatic activities in response to infection by Alternaria alternata evaluated in two stages of development in different tomato fruit varieties. Sci Hortic 112:42–50CrossRefGoogle Scholar
  10. Dashwood E, Fox R (1988) Infection of flowers and fruits of red raspberry by Botrytis cinerea. Plant Pathol 37:423–430CrossRefGoogle Scholar
  11. Davey MW, Auwerkerken A, Keulemans J (2007) Relationship of apple vitamin C and antioxidant contents to harvest date and postharvest pathogen infection. J Sci Food Agric 87:802–813CrossRefGoogle Scholar
  12. Deli J, Molnár P, Matus Z, Tóth G (2001) Carotenoid composition in the fruits of red paprika (Capsicum annuum var. lycopersiciforme rubrum) during ripening: biosynthesis of carotenoids in red paprika. J Agric Food Chem 49:1517–1523PubMedCrossRefGoogle Scholar
  13. Derckel JP, Audran JC, Haye B, Lambert B, Legendre L (1998) Characterization, induction by wounding and salicylic acid, and activity against Botrytis cinerea of chitinases and β-1,3-glucanases of ripening grape berries. Physiol Plant 104:56–64CrossRefGoogle Scholar
  14. Droby A, Lichter A (2004) Post-harvest Botrytis infection: etiology, development and management. In: Elad Y, Williamson B, Tudzynski P, Delen N (eds) Botrytis: biology, pathology and control. Kluwer Academic Press, Dordrecht, pp 349–367Google Scholar
  15. Eden M, Hill R, Beresford R, Stewart A (1996) The influence of inoculum concentration, relative humidity, and temperature on infection of greenhouse tomatoes by Botrytis cinerea. Plant Pathol 45:795–806CrossRefGoogle Scholar
  16. Eissa HA, Mostafa В, Hussein A (2007) Capsaicin content and quality characteristics in different local pepper varieties (Capsicum annum) and acid-brine pasteurized puree. J Food Technol 5:246–255Google Scholar
  17. Elad Y, Volpin H (1993) Reduced development of grey mould (Botrytis cinerea) in bean and tomato plants by calcium nutrition. J Phytopathol 139:146–156CrossRefGoogle Scholar
  18. Fallik E, Grinberg S, Alkalai S, Lurie S (1996) The effectiveness of postharvest hot water dipping on the control of grey and black moulds in sweet red pepper (Capsicum annuum). Plant Pathol 45:644–649CrossRefGoogle Scholar
  19. Fourie J, Holz G (1994) Infection of plum and nectarine flowers by Botrytis cinerea. Plant Pathol 43:309–315CrossRefGoogle Scholar
  20. Goetz G, Fkyerat A, Métais N, Kunz M, Tabacchi R, Pezet R, Pont V (1999) Resistance factors to grey mould in grape berries: identification of some phenolics inhibitors of Botrytis cinerea stilbene oxidase. Phytochemistry 52:759–767CrossRefGoogle Scholar
  21. Halfon-Meiri A, Rylski I (1983) Internal mold caused in sweet pepper by Alternaria alternata: fungal ingress. Phytopathology 73:67–70CrossRefGoogle Scholar
  22. Horowitz S, Yarden O, Zveibil A, Freeman S (2004) Development of a robust screening method for pathogenicity of Colletotrichum spp. on strawberry seedlings enabling forward genetic studies. Plant Dis 88:845–851CrossRefGoogle Scholar
  23. Howard L, Talcott S, Brenes C, Villalon B (2000) Changes in phytochemical and antioxidant activity of selected pepper cultivars (Capsicum species) as influenced by maturity. J Agric Food Chem 48:1713–1720PubMedCrossRefGoogle Scholar
  24. Jarvis WR, Borecka H (1968) The susceptibility of strawberry flowers to infection by Borytis cinerea Pers. ex Fr. Hortic Res 8:147–154Google Scholar
  25. Keller M, Viret O, Cole FM (2003) Botrytis cinerea infection in grape flowers: defense reaction, latency, and disease expression. Phytopathology 93:316–322PubMedCrossRefGoogle Scholar
  26. Kim JS, Ahn J, Lee SJ, Moon BK, Ha TY, Kim S (2011) Phytochemicals and antioxidant activity of fruits and leaves of paprika (Capsicum annuum L., var. Special) cultivated in Korea. J Food Sci 76:193–198CrossRefGoogle Scholar
  27. Lavy-Meir G, Barkai-Golan R, Kopeliovitch E (1989) Resistance of tomato ripening mutants and their hybrids to Botrytis cinerea. Plant Dis 73:967–922Google Scholar
  28. Lopez-Malo A, Alzamora S, Argaiz A (1998) Vanillin and pH synergistic effects on mold growth. J Food Sci 63:143–146CrossRefGoogle Scholar
  29. Maas JL (1998) Compendium of strawberry diseases. APS Press, St PaulGoogle Scholar
  30. McClellan W, Hewitt W (1973) Early Botrytis rot grapes: time of infection and latency of Botrytis cinerea Pers. in Vitis vinifera L. Phytopathology 63:1151–1157CrossRefGoogle Scholar
  31. McNicol R, Williamson B, Dolan A (1990) Effects of inoculation, wounding and temperature on post-harvest grey mould (Botrytis cinerea) of red raspberry. J Hortic Sci 65:157–165Google Scholar
  32. Michailides TJ, Elmer PAG (2000) Botrytis gray mold of kiwifruit caused by Botrytis cinerea in the United States and New Zealand. Plant Dis 84:208–223CrossRefGoogle Scholar
  33. Navarro JM, Flores P, Garrido C, Martinez V (2006) Changes in the contents of antioxidant compounds in pepper fruits at different ripening stages, as affected by salinity. Food Chem 96:66–73CrossRefGoogle Scholar
  34. Niklis N, Sfakiotakis E, Thanassoulopoulos C (1995) Ethylene production by Botrytis cinerea, kiwifruit and Botrytis rotted kiwifruit under several storage temperatures. Acta Horticult 444:733–738Google Scholar
  35. Ozdemir M, Floros JD (2004) Active food packaging technologies. Crit Rev Food Sci Nutr 44:185–193PubMedCrossRefGoogle Scholar
  36. Pezet R, Viret O, Perret C, Tabacchi R (2003) Latency of Botrytis cinerea Pers.: Fr. and biochemical studies during growth and ripening of two grape berry cultivars, respectively susceptible and resistant to grey mould. J Phytopathol 151:208–214CrossRefGoogle Scholar
  37. Pham NTT (2007) Ripening behaviour of capsicum (Capsicum annuum L.) fruit. PhD thesis, University of Adelaide, South Australia, 149 pagesGoogle Scholar
  38. Salzman RA, Tikhonova I, Bordelon BP, Hasegawa PM, Bressan RA (1998) Coordinate accumulation of antifungal proteins and hexoses constitutes a developmentally controlled defense response during fruit ripening in grape. Plant Physiol 117:465PubMedCrossRefGoogle Scholar
  39. Staats M, Van Baarlen P, Van Kan JAL (2005) Molecular phylogeny of the plant pathogenic genus Botrytis and the evolution of host specificity. Mol Biol Evol 22:333–346PubMedCrossRefGoogle Scholar
  40. Tao S, Zhang S, Tsao R, Charles MT, Yang R, Khanizadeh S (2010) In vitro antifungal activity and mode of action of selected polyphenolic antioxidants on Botrytis cinerea. Arch Phytopathol Plant Protect 43:1564–1578CrossRefGoogle Scholar
  41. Terry LA, Joyce DC, Adikaram NKB, Khambay BPS (2004) Preformed antifungal compounds in strawberry fruit and flower tissues. Postharvest Biol Technol 31:201–212CrossRefGoogle Scholar
  42. Utkhede RS, Mathur S (2005) Biological and chemical control of fruit rot in greenhouse sweet peppers (Capsicum annum L.) caused by Fusarium subglutinans. J Biol Sci 5:610–615CrossRefGoogle Scholar
  43. Villavicencio L, Blankenship SM, Sanders DC, Swallow WH (1999) Ethylene and carbon dioxide production in detached fruit of selected pepper cultivars. J Am Soc Hortic Sci 124:402–406Google Scholar
  44. Volpin H, Elad Y (1991) Influence of calcium nutrition on susceptibility of rose flowers to Botrytis blight. Phytopathology 81:1390–1394CrossRefGoogle Scholar
  45. Williamson B, McNicol R, Dolan A (1987) The effect of inoculating flowers and developing fruits with Botrytis cinerea on post-harvest grey mould of red raspberry. Ann Appl Biol 111:285–294CrossRefGoogle Scholar
  46. Williamson B, Tudzynski B, Tudzynski P, Van Kan JAL (2007) Botrytis cinerea: the cause of grey mould disease. Mol Plant Pathol 8:561–580PubMedCrossRefGoogle Scholar
  47. Wurms K (2005) Susceptibility to Botrytis cinerea, and curing-induced responses of lytic enzymes and phenolics in fruit of two kiwifruit (Actinidia) cultivars. N Z J Crop Hortic Sci 33:25–34CrossRefGoogle Scholar

Copyright information

© Australasian Plant Pathology Society Inc. 2013

Authors and Affiliations

  • Thong D. Le
    • 1
  • Glenn McDonald
    • 1
  • Eileen S. Scott
    • 1
  • Amanda J. Able
    • 1
  1. 1.School of Agriculture, Food and WineThe University of Adelaide, Waite Research InstituteGlen OsmondAustralia

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