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Australasian Plant Pathology

, Volume 41, Issue 5, pp 483–491 | Cite as

Fusarium circinatum and pitch canker of Pinus in Colombia

  • E. T. Steenkamp
  • C. A. Rodas
  • M. Kvas
  • M. J. Wingfield
Article

Abstract

Pitch canker, caused by the ascomycete fungus Fusarium circinatum, infects a wide range of Pinus species. The pathogen has a global distribution and limits plantation productivity wherever susceptible Pinus species are commercially cultivated. During 2005–2007, symptoms typical of those associated with F. circinatum were observed in Colombia on nursery seedlings of P. maximinoi, P. tecunumanii and P. patula, as well as established P. patula and P. kesiya trees in plantations. Symptoms on seedlings included collar and root disease while shoot dieback and resinous stem cankers were found on trees in plantations. The aim of this study was to isolate and identify the causal agent of these symptoms and to evaluate the relative tolerance of various families of Pinus species commonly grown in Colombia. By making use of morphology and DNA-based methods, as well as pathogenicity tests on P. patula seedlings, it was possible to show that the symptoms observed in the nursery and field were caused by F. circinatum. Furthermore, the results of pathogenicity tests with two virulent isolates of the pathogen indicated that P. tecunumanii from low-elevation sources and P. maximinoi are significantly more tolerant to infection by F. circinatum than P. tecunumanii from high-elevation sources and P. patula. These results show that there is substantial opportunity to avoid losses due to infection by F. circinatum through deployment of resistant planting stock.

Keywords

Fusarium circinatum Pitch canker Pathogenicity DNA-based diagnostics Pinus species 

Notes

Acknowledgements

We thank Smurfit Kappa Cartón de Colombia, the members of the Tree Protection Cooperative Programme (TPCP) and University of Pretoria, as well as the National Research Foundation (NRF) and the THRIP support programme of the Department of Trade and Industry in South Africa for financial support. We also thank Liliana Perafan and Mauricio Zapata of Smurfit Kappa Cartón de Colombia for their assistance with statistical analyses.

References

  1. Alonso R, Bettucci L (2009) First report of the pitch canker fungus Fusarium circinatum affecting Pinus taeda seedlings in Uruguay. Australas Plant Dis Notes 4:91–92Google Scholar
  2. Arvanitis LG, Godbee JF Jr, Porta I (1984) Pitch canker impact on volume growth: a case study in slash pine plantations. South J Appl For 8:43–47Google Scholar
  3. Blakeslee GM, Oak SW (1979) Significant mortality associated with pitch canker infection of slash pine in Florida. Plant Dis Rep 63:1023–1025Google Scholar
  4. Benson DA, Karsch-Mizrachi I, Lipman DJ, Ostell J, Sayers EW (2011) GenBank. Nucleic Acids Res 39(Database issue):D32–D37PubMedCrossRefGoogle Scholar
  5. Bethune JE, Hepting GH (1963) Pitch canker damage to south Florida slash pine. J For 61:517–522Google Scholar
  6. Bragança H, Diogo E, Moniz F, Amaro P (2009) First report of pitch canker on pines caused by Fusarium circinatum in Portugal. Plant Dis 93:1079CrossRefGoogle Scholar
  7. Britz H, Coutinho TA, Wingfield MJ, Marasas WFO, Gordon TR, Leslie JF (1999) Fusarium subglutinans f.sp. pini represents a distinct mating population in the Gibberella fujikuroi species complex. Appl Environ Microbiol 65:1198–1201PubMedGoogle Scholar
  8. Britz H, Coutinho TA, Gordon TR, Wingfield MJ (2001) Characterisation of the pitch canker fungus, Fusarium circinatum, from Mexico. S Afr J Bot 67:609–614Google Scholar
  9. Britz H, Coutinho TA, Wingfield MJ, Marasas WFO (2002) Validation of the description of Gibberella circinata and morphological differentiation of the anamorph Fusarium circinatum. Sydowia 54:9–22Google Scholar
  10. Carlucci A, Colatruglio L, Frisullo S (2007) First report of pitch canker caused by Fusarium circinatum on Pinus halepensis and P. pinea in Apulia (Southern Italy). Plant Dis 91:1683CrossRefGoogle Scholar
  11. Coutinho TA, Steenkamp ET, Mongwaketsi K, Wilmot M, Wingfield MJ (2007) First outbreak of pitch canker in a South African pine plantation. Australas Plant Pathol 36:256–261CrossRefGoogle Scholar
  12. Crous JW (2005) Post establishment survival of Pinus patula in Mpumalanga, 1 year after planting. South Afr For J 205:3–11Google Scholar
  13. Dwinell LD, Barrows-Broaddus J, Kuhlman EG (1985) Pitch canker: a disease complex of southern pines. Plant Dis 69:270–276CrossRefGoogle Scholar
  14. Fisher NL, Burgess LW, Toussoun TA, Nelson PE (1982) Carnation leaves as a substrate and for preserving cultures of Fusarium species. Phytopathology 72:151–153CrossRefGoogle Scholar
  15. Geiser DM, Jiménez-Gasco MM, Kang S, Makalowski I, Veeraraghavan N, Ward TJ, Zhang N, Kuldau GA, O’Donnell K (2004) FUSARIUM-ID v. 1.0: a DNA sequence database for identifying Fusarium. Eur J Plant Pathol 110:473–479CrossRefGoogle Scholar
  16. Geiser DM, Ivey ML, Hakiza G, Juba JH, Miller SA (2005) Gibberella xylarioides (anamorph: Fusarium xylarioides), a causative agent of coffee wilt disease in Africa, is a previously unrecognized member of the G. fujikuroi complex. Mycologia 97:191–201PubMedCrossRefGoogle Scholar
  17. Gordon TR, Okamoto D, Storer AJ, Wood DL (1998a) Susceptibility of five landscape pines to pitch canker disease, caused by Fusarium subglutinans f. sp. pini. HortSci 33:868–871Google Scholar
  18. Gordon TR, Wikler KR, Clark SL, Okamoto D, Storer AJ, Bonello P (1998b) Resistance to pitch canker disease, caused by Fusarium subglutinans f. sp. pini, in Monterey pine (Pinus radiata). Plant Pathol 47:706–711Google Scholar
  19. Gordon TR, Storer AJ, Wood DL (2001) The pitch canker epidemic in California. Plant Dis 85:1128–1139CrossRefGoogle Scholar
  20. Gordon TR, Leveau JHJ (2010) Plant pathology: a story about biology. Annu Rev Phytopathol 48:292–309CrossRefGoogle Scholar
  21. Guindon S, Gascuel O (2003) PHYML – A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol 52:696–704PubMedCrossRefGoogle Scholar
  22. Guerra-Santos JJ (1999) Pitch canker on Monterey pine in Mexico. In ‘Current and potential impacts of pitch canker in radiata pine. Proceedings of the IMPACT Monterey workshop, California, USA, 30 November to 3 December 1998’. Forestry and Forest Products No.112. (Eds ME Devey, AC Matheson, TR Gordon) pp 58–61. (CSIRO: Canberra)Google Scholar
  23. Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98Google Scholar
  24. Hepting GH, Roth ER (1946) Pitch canker, a new disease of some southern pines. J For 44:724–744Google Scholar
  25. Hepting GH, Roth ER (1953) Host relations and spread of the pine pitch canker disease. Phytopathology 43:475Google Scholar
  26. Hodge GR, Dvorak WS (2000) Differential responses of Central American and Mexican pine species and Pinus radiata to infection by the pitch canker fungus. New Forests 19:241–258CrossRefGoogle Scholar
  27. Hodge GR, Dvorak WS (2007) Variation in pitch canker resistance among provenances of Pinus patula and Pinus tecunumanii from Mexico and Central America. New Forests 33:193–206CrossRefGoogle Scholar
  28. IDEAM (Instituto de Hidrologia, Meteorologia y Estudios Ambientales) (2009) Informe Anual sobre el estado del medio ambiente y los recursos naturales renovables en Colombia: Bosques-2009, p 236Google Scholar
  29. Iturritxa E, Ganley RJ, Wright J, Heppe E, Steenkamp ET, Gordon TR, Wingfield MJ (2011) A genetically homogenous population of Fusarium circinatum causes pitch canker of Pinus radiata in the Basque Country, Spain. Fungal Biol 115:288–295PubMedCrossRefGoogle Scholar
  30. Katoh K, Misawa K, Kuma K, Miyata T (2002) MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Res 30:3059–3066PubMedCrossRefGoogle Scholar
  31. Kobayashi T, Muramoto M (1989) Pitch canker of Pinus luchuensis, a new disease of Japanese forests. Forest Pests 40:169–173Google Scholar
  32. Kvas M, Steenkamp ET, Al Dawi AO, Deadman ML, Al Jahwari AA, Marasas WFO, Wingfield BD, Ploetz RC, Wingfield MJ (2008) Fusarium mangiferae associated with mango malformation in the Sultanate of Oman. Eur J Plant Pathol 121(1):95–199Google Scholar
  33. Lambrechts L, Fellous S, Koella JC (2006) Coevolutionary interactions between host and parasite genotypes. Trends Parasitol 22:12–16PubMedCrossRefGoogle Scholar
  34. Landeras E, García P, Fernández Y, Braña M, Fernández-Alonso O, Méndez-Lodos S, Pérez-Sierra A, Léon M, Abad-Campos P, Berbegal M, Berltrán R, Gárcia-Jiménez J, Armengol J (2005) Outbreak of pitch canker caused by Fusarium circinatum on Pinus spp. in northern Spain. Plant Dis 89:1015CrossRefGoogle Scholar
  35. Leibing C, van Zonneveld M, Jarvis A, Dvorak W (2009) Adaptation of tropical and subtropical pine plantation to climate change: realignment of Pinus patula and Pinus tecunumanii genotypes to 2020 planting site climates. Scand J For Res 24:483–493CrossRefGoogle Scholar
  36. Lee JK, Lee SH, Yang SI, Lee YW (2000) First report of pitch canker disease on Pinus rigida in Korea. Plant Pathol J 16:52–54Google Scholar
  37. Leslie JF, Summerell BA (2006) The Fusarium laboratory manual. Blackwell Professional, Ames, IowaCrossRefGoogle Scholar
  38. Matheson AC, Devey ME, Gordon TR, Werner W, Vogler DR, Balocchi C, Carson MJ (2006) Heritability of response to inoculation by pine pitch canker of seedlings of radiata pine. Aust For 69:101–106Google Scholar
  39. Mendell BC, De La Torre R, Sydor T (2006) Timberland investments in South America: a profile of Colombia. Timber Mart-South Market News Quarterly 3rd Quarter, pp 13–14Google Scholar
  40. Mitchell RG, Zwolinski J, Jones NB, Coutinho TA (2004) The effect of applying prophylactic measures on the post-planting survival of Pinus patula in South Africa. South Afr For J 200:51–59Google Scholar
  41. Mitchell RG, Steenkamp ET, Coutinho TA, Wingfield MJ (2011) The pitch canker fungus, Fusarium circinatum: implications for South African forestry. Southern Forests 73:1–13CrossRefGoogle Scholar
  42. Nirenberg HI (1976) Untersuchungen über die morphologische und biologische Differenzierung in der Fusarium-Sektion Liseola. Mitteilungen aus der Biologischen Bundesanstalt für Landund Forstwirtschaft 169:1–117Google Scholar
  43. Nirenberg HI, O’Donnell K (1998) New Fusarium species and combinations within the Gibberella fujikuroi species complex. Mycologia 90:434–458CrossRefGoogle Scholar
  44. O’Donnell K, Cigelnik E (1997) Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus Fusarium are nonorthologous. Mol Phylogenet Evol 7:103–116PubMedCrossRefGoogle Scholar
  45. O’Donnell K, Cigelnik E, Nirenberg HI (1998a) Molecular systematics and phylogeography of the Gibberella fujikuroi species complex. Mycologia 90:465–493CrossRefGoogle Scholar
  46. O’Donnell K, Kistler HC, Cigelnik E, Ploetz RC (1998b) Multiple evolutionary origins of the fungus causing Panama disease of banana: concordant evidence from the nuclear and mitochondrial gene genealogies. Proc Natl Acad Sci USA 95:2044–2049PubMedCrossRefGoogle Scholar
  47. Ronquist F, Heuelsenbeck JP (2003) MrBayes: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574PubMedCrossRefGoogle Scholar
  48. Santos JJG, Tovar DB (1991) Algunos aspectos sobre el cancro resinoso de los pinos. In ‘VI Simposio Nacional Sobre Parasitologia orestal, October 1991’. p 31. (Unidad de Xcongresos del Colegio de Postgraduados Montecillos: Edo, Mexico)Google Scholar
  49. SAS Institute (2009) SAS System for Windows Version 9.2. SAS Institute Inc., Cary, NC, USAGoogle Scholar
  50. Steenkamp ET, Wingfield BD, Coutinho TA, Wingfield MJ, Marasas WFO (1999) Differentiation of Fusarium subglutinans f. sp. pini by histone gene sequence data. Appl Environ Microbiol 65:3401–3406PubMedGoogle Scholar
  51. Storer AJ, Gordon TR, Clark SL (1998) Association of the pitch canker fungus, Fusarium subglutinans f. sp. pini with Monterey pine seeds, and seedlings in California. Plant Pathol 47:649–656CrossRefGoogle Scholar
  52. Thompson JN, Burdon JJ (1992) Gene-for-gene coevolution between plants and parasites. Nature 360:121–126CrossRefGoogle Scholar
  53. Wikler KR, Gordon TR (2000) An initial assessment of genetic relationships among populations of Fusarium circinatum in different parts of the world. Can J Bot 78:709–717Google Scholar
  54. Wingfield MJ, Wingfield BD, Coutinho TA, Viljoen A, Britz H, Steenkamp ET (1999) Pitch canker: a South African perspective. In ‘Current and potential impacts of pitch canker in radiata pine. Proceedings of the IMPACT Monterey workshop, California, USA, 30 November to 3 December 1998’. (Eds ME Devey, AC Matheson, TR Gordon) pp 62–69. (CSIRO Forestry and Forest Products No. 112: Canberra)Google Scholar
  55. Wingfield MJ, Coutinho TA, Roux J, Wingfield BD (2002a) The future of exotic plantation forestry in the tropics and southern hemisphere: lessons from pitch canker. South Afr For J 195:79–82Google Scholar
  56. Wingfield MJ, Jacobs A, Coutinho TA, Ahumada R, Wingfield BD (2002b) First report of the pitch canker fungus, Fusarium circinatum, on pines in Chile. Plant Pathol 51:397CrossRefGoogle Scholar
  57. Wingfield MJ, Hammerbacher A, Ganley RJ, Steenkamp ET, Gordon TR, Wingfield BD, Coutinho TA (2008) Pitch canker caused by Fusarium circinatum – a growing threat to the pine plantations and forests worldwide. Australas Plant Pathol 37:319–334CrossRefGoogle Scholar
  58. Woo K-S, Yoon J-H, Han S-U, Kim C-S (2010) Assessment of pathogenic variation against pitch canker pathogen, Fusarium circinatum in Pinus thunbergii and responses of natural selection Pinus x rigitaeda to branch inoculation in a seed orchard. Plant Pathol J 26:299–305CrossRefGoogle Scholar

Copyright information

© Australasian Plant Pathology Society Inc. 2012

Authors and Affiliations

  • E. T. Steenkamp
    • 1
  • C. A. Rodas
    • 2
  • M. Kvas
    • 1
  • M. J. Wingfield
    • 1
  1. 1.Department of Microbiology and Plant Pathology, Tree Protection Co-operative Programme, Forestry and Agricultural Biotechnology Institute (FABI)University of PretoriaPretoriaSouth Africa
  2. 2.Smurfit Kappa Cartón de Colombia, Investigación ForestalCali, ValleColombia

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