Indian Pediatrics

, Volume 51, Issue 9, pp 727–729 | Cite as

Molecular copro-prevalence of Cryptosporidium in Egyptian children and evaluation of three diagnostic methods

  • Mona M. Fathy
  • Noha M. Abdelrazek
  • Fayza A. Hassan
  • Ayman A. El-Badry
Research Brief

Abstract

Objective

To determine molecular prevalence of Cryptosporidium in a cohort of Egyptian children and compare three diagnostic tests.

Methods

Stool samples from children with diarrhea (n=150) and from apparently healthy children (n=100) were examined for Cryptosporidium using microscopy, enzyme linked immuosorbant assay (ELISA) and nested polymerase chain reaction (nPCR).

Results

nPCR detected Cryptosporidium in 22.4% of children. Acid-fast stain and ELISA showed false negativity but 100% specificity with nPCR as gold standard.

Conclusion

Cryptosporidium is a common cause of diarrhea in children in Egypt.

Keywords

Diarrhea Etiology ELISA Nested PCR 

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Copyright information

© Indian Academy of Pediatrics 2014

Authors and Affiliations

  • Mona M. Fathy
    • 1
  • Noha M. Abdelrazek
    • 2
  • Fayza A. Hassan
    • 1
  • Ayman A. El-Badry
    • 2
  1. 1.Departments of Clinical and Chemical PathologyCairo UniversityCairoEgypt
  2. 2.Departments of Medical Parasitology, Kasr Al-Ainy Faculty of MedicineCairo UniversityCairoEgypt
  3. 3.Medical Parasitology Department, Kasr Al-Ainy Faculty of MedicineCairo UniversityEl Manial, CairoEgypt

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