Indian Pediatrics

, Volume 47, Issue 9, pp 761–769 | Cite as

Impact of two regimens of vitamin D supplementation on calcium — vitamin D — PTH axis of schoolgirls of Delhi

  • Raman K. MarwahaEmail author
  • Nikhil Tandon
  • Neha Agarwal
  • Seema Puri
  • Rashmi Agarwal
  • Satveer Singh
  • Kalaivani Mani
Research Paper



To determine the efficacy of supplementation with oral vitamin D3 (cholecalciferol) on bone mineral biochemical parameters of school-going girls.


Government school (government-aided) and Private school (fee paying) in Delhi.


Randomized controlled trial.


Cholecalciferol granules (60,000 IU) orally with water, either once in two months (two-monthly D3 group) or once a month (one-monthly D3 group) for one year.


290 healthy schoolgirls (6–17 y), 124 from lower socioeconomic strata (LSES) (attending government schools) and 166 from upper socioeconomic strata (USES) (attending private schools).

Outcome measures

Serum 25(OH)D, calcium, phosphorus, parathyroid hormone, and alkaline phosphatase levels at 6 and 12 months after start of supplementation.


At baseline, 93.7% schoolgirls were vitamin D deficient [25(OH)D<50 nmol/L]. While significant increase in serum calcium and decrease in alkaline phosphatase levels was noted in both groups with both interventions, PTH response was inconsistent. In LSES subjects, twomonthly D3 and one-monthly D3 supplementation resulted in a significant increase in serum 25(OH)D levels by 8.3 nmol/L and 11.0 nmol/L, respectively at 6 months (P<0.05). Similarly, the increase in the two intervention arms in USES subjects was 10.5 nmol/L and 16.0 nmol/L, respectively (P<0.05). In both groups, this increase in serum 25(OH)D levels persisted at 12 months (P<0.05). Despite supplementation with 60,000 IU of Vitamin D3 (monthly or two-monthly), only 47% were vitamin D sufficient at the end of one year.


60,000 IU of cholecalciferol, monthly or two-monthly, resulted in a significant increase in serum 25(OH)D levels in vitamin D deficient schoolgirls.

Key words

Cholecalciferol India Supplementation Schoolgirls Vitamin D 


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Copyright information

© Indian Academy of Pediatrics 2010

Authors and Affiliations

  • Raman K. Marwaha
    • 1
    • 5
    Email author
  • Nikhil Tandon
    • 2
  • Neha Agarwal
    • 3
  • Seema Puri
    • 3
  • Rashmi Agarwal
    • 1
  • Satveer Singh
    • 1
  • Kalaivani Mani
    • 4
  1. 1.Department of Endocrinology and Thyroid ResearchInstitute of Nuclear Medicine and Allied Sciences (INMAS)New DelhiIndia
  2. 2.Department of Endocrinology and MetabolismAll India Institute of Medical Sciences (AIIMS)New DelhiIndia
  3. 3.Department of Foods and Nutrition, Institute of Home EconomicsUniversity of DelhiNew DelhiIndia
  4. 4.Department of BiostatisticsAll India Institute of Medical SciencesNew DelhiIndia
  5. 5.Department of Endocrinology and Thyroid Research CentreINMASDelhiIndia

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