Withania somnifera Reverses Transactive Response DNA Binding Protein 43 Proteinopathy in a Mouse Model of Amyotrophic Lateral Sclerosis/Frontotemporal Lobar Degeneration
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Abnormal cytoplasmic mislocalization of transactive response DNA binding protein 43 (TARDBP or TDP-43) in degenerating neurons is a hallmark of amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration with ubiquitin-positive inclusions (FTLD-U). Our previous work suggested that nuclear factor kappa B (NF-κB) may constitute a therapeutic target for TDP-43-mediated disease. Here, we investigated the effects of root extract of Withania somnifera (Ashwagandha), an herbal medicine with anti-inflammatory properties, in transgenic mice expressing a genomic fragment encoding human TDP-43A315T mutant. Ashwagandha extract was administered orally to hTDP-43A315T mice for a period of 8 weeks starting at 64 and 48 weeks of age for males and females, respectively. The treatment of hTDP-43A315T mice ameliorated their motor performance on rotarod test and cognitive function assessed by the passive avoidance test. Microscopy examination of tissue samples revealed that Ashwagandha treatment of hTDP-43A315T mice improved innervation at neuromuscular junctions, attenuated neuroinflammation, and reduced NF-κB activation. Remarkably, Ashwagandha treatment reversed the cytoplasmic mislocalization of hTDP-43 in spinal motor neurons and in brain cortical neurons of hTDP-43A315T mice and it reduced hTDP-43 aggregation. In vitro evidence is presented that the neuronal rescue of TDP-43 mislocalization may be due to the indirect effect of factors released from microglial cells exposed to Ashwagandha. These results suggest that Ashwagandha and its constituents might represent promising therapeutics for TDP-43 proteinopathies.
KeywordsAmyotrophic lateral sclerosis Withania somnifera TDP-43 NF-κB.
Transactive response DNA binding protein was originally described as a regulatory element involved in modulating HIV-1 gene expression . The 414 amino acid-containing protein has a molecular weight of 43 kDa (hence it is commonly referred to as TDP-43), and structurally consists of 2 RNA recognition motifs, a nuclear localization sequence, a nuclear export domain, and a glycine-rich C-terminal domain . TDP-43 is predominantly a nuclear protein, even though it is capable of shuttling between the nucleus and cytoplasm—a process partly regulated by nuclear localization signal and nuclear export signal motifs . TDP-43 is a DNA/RNA binding protein and it is involved in regulating RNA transcription, splicing, trafficking, and microRNA biogenesis . Its involvement in neurodegenerative disorders was first reported in 2006 when hyperphosphorylated, ubiquitinated, and cleaved C-terminal fragments of the protein were detected from postmortem brain and spinal cord of patients suffering from frontotemporal lobar degeneration with ubiquitin-positive inclusions (FTLD-U) and amyotrophic lateral sclerosis (ALS) [5, 6]. TDP-43 has been shown to be prone to aggregation , and neuronal and glial TDP-43 inclusions have since been reported from >95% of sporadic ALS cases. Protein cleavage, aggregation, and neurotoxicity enhancing mutations in the TARDBP have also been reported from both familial and sporadic ALS cases .
Cellular levels of TDP-43 appear to be tightly regulated. The protein has the intrinsic property of autoregulating its RNA level by binding with the 3' untranslated region leading to the excision of an intron, thereby resulting in its degradation by nonsense-mediated RNA decay [9, 10]. However, in some human patients it has been demonstrated that TDP-43 levels are elevated [11, 12], suggesting that disease-associated TDP-43 aggregates disrupt its self-regulation, thereby contributing to the pathogenesis. Mislocalization of nuclear TDP-43 into the cytoplasm is also an early event in disease pathology, resulting in neurotoxicity . However, whether nuclear depletion, constituting a “loss of function” of the protein, or its accumulation in cytoplasm constituting a “novel gain of toxic function” plays the key role in the disease has been a matter of debate [13, 14]. Nonetheless, insights from few recent studies favor the “loss of nuclear function” hypothesis [15, 16, 17, 18, 19].
In a previous study, we provided evidence that nuclear factor kappa B (NF-κB) may constitute a therapeutic target in ALS pathogenesis with TDP-43 deregulation. We showed that TDP-43 binds to and acts as a coactivator of the P65 subunit of NF-κB . Postmortem spinal cord samples from sporadic ALS cases exhibited elevated levels of NF-κB mRNA when compared with age-matched controls and the p65 NF-κB protein displayed abnormal nuclear localization in neurons. In addition, treatment of transgenic mice expressing human TDP-43A315T mutant with Withaferin A, a NF-κB inhibitor, ameliorated disease phenotypes. Withaferin A is a component of the plant Withania somnifera dunal [or Ashwagandha (ASH)], a perennial plant belonging to the family Solanaceae  that has been in use for the last 4000 years in traditional Indian medical system (Ayurveda). The root of the plant reportedly contains 14 to 15 different alkaloids and about 40 structurally similar steroidal lactones (called withanolides) other than various carbohydrates and amino acids, in varying amounts . It reportedly has antimicrobial, anti-inflammatory, antineoplastic, antistress, cardioprotective, antidiabetic, and neuroprotective properties . The neuroprotective property of the plant extract or purified products from the plant has been demonstrated in multiple disease models such as Alzheimer’s disease, Parkinson’s disease, Huntington’s disease, and spinal cord injuries . The current study was designed to evaluate the efficacy of Ashwagandha (ASH) root extract to ameliorate behavioral and pathological phenotypes in a transgenic mouse model of ALS/FTLD exhibiting TDP-43 proteinopathy .
Materials and Methods
Preparation of ASH Root Extract
Withania somnifera (ASH) root, rich in various withanolides and alkaloids, was provided to us in dried powder form by Valeant Pharmaceuticals International Inc (Quebec, Canada). The product is an 11:1 extract from the plant root.
ALS/FTLD Mouse Model and Treatment Paradigm
A transgenic mouse line bearing human genomic fragment encoding TDP-43 with A315T mutation was generated previously by us . Animals were randomly distributed in either ASH-treated (n = 28) or Vehicle (Veh)-treated (n = 24) groups. The average age of the male mice at the beginning of treatment was 445 ± 0.45 days and that of female mice was 338 ± 3.2 days. Birth records of all the mice used for the experiments are provided in Figure S1. Mice in the ASH group were fed 5 mg root powder (by gavage) as a suspension in 200 μl sterile buffered saline every alternate day, for 8 or 16 weeks. Animals in the Veh group received equal volumes of buffered saline only for the same durations. This treatment had no effect on survival and body weight of the animals, and there were no outward manifestation of deleterious side effects. The Animal Care Ethics Committee of Université Laval approved all in vivo experimental protocols. Experiments were carried out in accordance with the Guide to the Care and Use of Experimental Animals of the Canadian Council on Animal Care.
Rotarod Performance Test
To test motor coordination in treated and untreated mice they were allowed to run on an accelerating rotarod at 3 rpm speed with 0.25 rpm/s acceleration. Mice were subjected to 3 trials per session every week and the longest latency to fall from the rotating rod was recorded. The maximum cut-off limit was set for 3 min.
Passive Avoidance Test
One-trial passive avoidance test was performed as described earlier , with minor modifications. The latency time for mice to enter the dark compartment was measured, with a 5-min cut-off.
Immunofluorescence Microscopy and Image Analysis
List of antibodies used for Western blots (WB) and immunofluorescence (IF)
Dilution for WB
Dilution for IF
Millipore (Temecula CA, USA)
Santa Cruz (Santa Cruz, CA, USA)
Cell Signaling Technologies (Danvers, MA, USA)
Glyceraldehyde 3-phosphate dehydrogenase
Glial fibrillary acidic protein
Cell Signaling Technologies
Hemagglutinin antigen HA
Roche Applied Sciences (Penzberg, Germany)
Human transactive response DNA binding protein 43 (TDP-43; clone 2E2-D3)
Abnova (Taipei City, Taiwan)
Wako Chemicals (Richmond, VA, USA)
Inducible nitric oxide synthase
BD Biosciences (San Jose, CA, USA)
Cell Signaling Technologies
Nuclear factor kappa B (NF-κB)
Abcam (Cambridge, UK)
Proteintech (Chicago, IL, USA)
Phospho-NF-κB (Ser 536)
Cell Signaling Technologies,
Synaptic vesicle protein-2 SV-2
Developmental Studies Hybridoma Bank (Mt. Prospect, IA, USA)
Tumor necrosis factor-α
Stem Cell Technologies (Vancouver, Canada)
Neuromuscular Junction Staining
Twenty-μm-thick cryosections of mouse gastrocnemius muscle were used to stain for neuromuscular junctions (NMJs). Presynaptic connections were stained using neurofilament-H and synaptic vesicle protein 2 (SV2). Tetramethylrhodamine-conjugated α-bungarotoxin (1:100; Sigma-Aldrich, St. Louis, MO, USA) was used to label acetylcholine receptors located at the subsynaptic membrane. Montages of z-stack images were captured using a Zeiss Apotome microscope and the images were processed using ImageJ and Adobe Photoshop. NMJs were classified as fully innervated, partially innervated, or denervated, based on the extent of overlap of α-bungarotoxin and neurofilament-H+SV2 staining.
Protein Extraction Protocols
Spinal cords were excised from anesthetized mice after perfusion with ice-cold 0.9% saline. Whole protein lysates were extracted by methods previously described . Cytosolic and nuclear fractions from cultured cells were also prepared as per methods described elsewhere . Purity of the fractions was confirmed by the presence of cytosolic glyceraldehyde 3-phosphate dehydrogenase or nuclear P84 proteins.
Immunoblot analysis were performed from samples containing equal amount of protein (quantified by Bio-Rad Protein assay; Bio-Rad, Hercules, CA, USA) by methods previously described . Details of antibodies used are represented in Table 1.
Generation of Stable NSC34-hTDPA315T Cell Line
For in vitro experiments, NSC34 (a murine neuroblastoma/spinal cord hybrid) cell line was used. Mammalian expression vector plasmid pCMV–TDP-43 with point mutation A315T and a HA tag was generated previously . Cells were transfected with the pCMV–TDP-43A315T plasmid using Lipofectamine 2000 reagent (Life Technologies, Carlsbad, CA, USA) and were subsequently selected in Dulbecco’s modified eagle medium (DMEM) containing 375 μg/ml Geneticin (G418; Life Technologies). For further experiments, selected clones were amplified, checked for protein expression, and propagated. Prior to experimentation, these cells were differentiated as per the published protocol .
Luciferase Assay to Check p65 Activation in Microglia
Mouse microglial cell line BV2 was stably transfected with pGL4.32[luc2P/NF-κB–RE/Hygro] plasmid DNA (Promega, Madison, WI, USA). The pGL4.32[luc2P/NF-κB–RE/Hygro] vector contains 5 copies of an NF-κB response element that drives transcription of the luciferase reporter gene luc2P. The stable cell line was maintained in DMEM supplemented with 10% fetal bovine serium and 100 μg/ml hygromycin. Cells (5 × 104 per well) were seeded in 24-well plates. The cells were stimulated with 100 ng/ml bacterial lipopolysaccharide (LPS) for 3 h, after which the media was removed, wells washed with 1× PBS, followed by cell lysis using Glo Lysis buffer (Promega). Luciferase activity was measured using the Bright-Glo Luciferase assay system (Promega), according to the manufacturer’s instructions. To test the efficacy of ASH on reducing LPS-induced P65 activity, cells were treated with varying concentrations of ASH extract in dimethyl sulfoxide (DMSO; 1 μg, 10 μg, 100 μg, 250 μg, and 500 μg per ml) for 3 h post-LPS treatment, followed by luciferase assay. Control studies were done with cells treated with ASH alone. Results were expressed as mean of luciferase activity/μg cellular protein from at least 6 wells in each treatment condition.
Cell Survival Assay
Cell viability post-ASH treatment was assessed using [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] MTS assay, as per the manufacturer’s instructions (Promega). BV2 was seeded onto 96-well plates at a density of 104 cells/well. The treatment paradigm was similar to that explained for luciferase assay. Postincubation with MTS reagent, the absorbance, reflecting the reduction of MTS by viable cells, was determined at 490 nm using an EnSpire 2300 Multilabel reader (Perkin Elmer, Waltham, MA, USA). Values were expressed as a percentage relative to those obtained in controls.
To test the effect of ASH extract on viability of NSC34–hTDP-43A315T, cells were seeded in a 96-well plate and subsequently treated with 250, 100, 10, and 1 μg/ml ASH extract for 6 h. Values were expressed as a percentage relative to those obtained in controls.
In Vitro Model of Inducing hTDP-43 Mislocalization
To induce mislocalization of nuclear TDP-43 into the cytoplasm in NSC34–hTDP-43A315T cells, cells were subjected to excitotoxic (2 mM glutamate in buffer containing 125 mM NaCl, 10 mM CaCl2 5.9 mM KCl, 11.6 mM HEPES, and 11.5 mM glucose; pH 7.4), inflammatory [40 ng/ml of recombinant tumor necrosis factor (TNF)-α], or oxidative stimuli (50 μM) for varying time points . Cells were collected, washed once in ice-cold 1× PBS, and subjected to fractionation for separation of cytosolic and nuclear fractions as described above. Immunoblotting was performed with the protein samples to determine the level TDP-43 in each fraction. Untreated NSC34–hTDP-43A315T served as control.
Effect of Microglia-Conditioned Media on Glutamate-Stimulated NSC34–hTDP-43A315T
To test the role of soluble factors released from microglia on TDP-43 distribution in NSC34–hTDP-43A315T cells, BV2 cells were seeded at a density of 2 × 106 cells per 10-cm plate and cultured from 16–20 h in DMEM containing 10% FBS. Prior to treatment, the culture media was replaced with serum-free DMEM. Subsequently, the cells were treated with either LPS (100 ng/ml) or ASH (250 μg/ml dissolved in DMSO) and incubated for 4 h at 37 °C. Control BV2 cells were treated with an equal volume of DMSO only. After 4 h the culture supernatant was collected and centrifuged under sterile conditions at 2655 g for 5 min to precipitate cellular debris; this served as the conditioned media. NSC34–hTDP-43A315T cells that had been seeded separately into 3 other plates had been treated with glutamate (as described above) almost at the same time as BV2 treatment. Prior to addition of the conditioned media onto the NSC34–hTDP-43A315T cells, their glutamate-containing media were removed, plates were washed with sterile PBS, and finally incubated with the BV2-conditioned media and some fresh serum-free DMEM (at a ratio of 2:1) for 12 h. Postincubation, cells were collected and subjected to nuclear cytosolic fractionation. Subsequently, they were analyzed by immunoblotting to determine TDP-43 levels. As negative controls for this experiment, glutamate-treated NSC34–hTDP-43A315T cells were treated with BV2-conditioned media that had been heated at 70 °C to denature any soluble factors that may have been contained in them.
Culture and Treatment of Primary Mouse Microglia
Primary microglia were cultured from the brains of pups (postnatal day 6) of TDP-43A315T mutant mice. Brains were collected and placed in ice-cold PBS. Following mechanical dissociation, the brains were incubated in a 0.25% Trypsine-EDTA solution (Sigma-Aldrich) containing 250 K U/ml DNase I (Sigma-Aldrich). After centrifugation, the cell pellets were placed in T-75 cm2 flasks (Sarstedt, Nümbrecht, Germany) for 10 d at 37 °C, 5% CO2, in DMEM high-glucose media with 10% fetal bovine serum and antibiotic solution (Sigma-Aldrich). Genotyping carried out from tissues of the pups confirmed the identity of each culture. At confluence, the wild-type (WT), as well as transgenic microglia, were plated in 6-well plates at a concentration of 200,000 cells/well in serum containing media. Cells were incubated with granulocyte colony-stimulating factor 24 h later to allow adhesion. After 2 to 3 days cells were deemed suitable for experimentation.
Prior to treatment, cells were transferred to serum-free media. Microglia from WT mice or transgenic TDP-43A315T mice were challenged with LPS or DMSO for 6 h to elicit cytokine/chemokine release. One set of WT microglia was treated with LPS for 3 h followed by further addition and incubation with ASH extract in DMSO (250 μg/ml) for 3 h more. Postincubation, the media was collected, centrifuged at 1000 × g for 5 min to precipitate out any debris, and the resultant media was used for estimation of cytokine/chemokine release.
The cytokine expression profiles from primary microglia at different treatment conditions were performed with mouse cytokine antibody array kit (Raybio Mouse Inflammation Antibody Array 1, Cat#AAM- INF-1; RayBiotech, Norcross, GA, USA) as previously described . Cell culture media post-treatment were centrifuged at 300 × g to remove cellular debris and incubated with the array membranes overnight at 4 °C. After washing in with the buffer provided with the kit, membranes were incubated with biotin-conjugated antibodies overnight. Signal detection was performed according to the RayBiotech protocol, by exposing membranes to X-ray film (Biomax MR1; #8701302; Kodak, Rochester, NY, USA), and the obtained results analyzed using ImageJ software. Data are expressed in arbitrary units relative to appropriate positive controls.
Effect of Direct ASH Treatment on NSC34–hTDP-43A315T Cells
To evaluate if ASH can directly affect TDP-43 re-distribution, NSC34–hTDP-43A315T cells were treated with ASH at a concentration of 10 μg/ml, with or without glutamate pretreatment. TDP-43 levels were determined by immunoblotting from nuclear and cytoplasmic fractions of these cells.
Prism 5.0 (GraphPad, La Jolla, CA, USA) was used for all statistical analysis. Comparisons between 2 groups were done by unpaired two-tailed t test with Welch’s correction. Comparison between multiple groups was done by 1-way analysis of variance with Bonferroni’s post-test. A p-value up to 0.05 was considered significant.
ASH Extract Inhibits NF-κB Activation in Cultured Cells
Modulation of Cytokine/Chemokine Profile of Stimulated Primary Microglia Post-Treatment With ASH
In vitro, there was no significant difference between the secretory cytokine/chemokine profiles of WT or TDP-43A315T microglia, with or without LPS challenge (Fig. S2a). On treating WT microglia with LPS a significant upregulation was observed in the levels of FAS ligand (2.68-fold), interferon-γ (2-fold), interleukin (IL)-1β (2.06-fold; but not statistically significant), IL-6 (13.8-fold), IL-17A (2.21-fold), monocyte chemoattractant protein-1 (2-fold), macrophage inflammatory protein (MIP)-1α (4.5-fold) and MIP-1γ (1.6-fold), monokine induced by interferon-gamma (2.8-fold), the chemokine LIX (4.68-fold), and regulated on activation, normal T cell expressed and secreted (RANTES) (2.65-fold). On the contrary, IL-4 (0.52-fold) and chemokine (C motif) ligand 1 (0.6-fold) levels were found to decrease significantly following LPS challenge. However, ASH treatment of LPS-challenged cells resulted in significant downregulation in the levels of the upregulated cytokines/chemokines and upregulation of IL-4 and chemokine (C motif) ligand 1 levels. Interestingly, even though the levels of soluble TNF receptor R1 and R2 levels were found to be elevated after LPS challenge, there was no significant alteration in the level of secreted TNF-α (data not shown) (Fig. 1c–q).
Amelioration of Motor and Cognitive Performance in Transgenic Mice Expressing hTDP-43A315T
To further evaluate the effect of ASH on cognitive performance of hTDP-43A315T mice, we used the passive avoidance test. ASH treatment of hTDP-43A315T mice increased significantly the latency to enter the dark chamber, irrespective of sex (Fig. 2c, d). In contrast, the Veh-treated hTDP-43A315T mice were inept at recalling the unpleasant experience encountered in the dark chamber, thereby indicating cognitive defects.
Improvement of NMJ Innervation in hTDP-43A315T Mice
Attenuation of Neuroinflammation in hTDP-43A315T Mice
Morphologically, there was also a difference in microglial phenotypes of spinal cords from ASH- and Veh-treated hTDP-43A315T mice. Microglia, visualized by Iba-1 staining, showed a more reactive phenotype in Veh-treated mice compared with that in ASH-treated mice. Microglia in ASH-treated mice showed small soma with distal arborization (Fig. 4i–k); in contrast, microglia in Veh-treated mice showed an increase in soma size with multiple short thick processes branching out (Fig. 4l–n). Iba-1 signal intensity analysis also corroborated a lesser intensity of staining in ASH-treated animals (p < 0.05) (Fig. 4h). Ym-1 and arginase-1 are well-reported phenotypic markers for M2a microglia. In our samples, we saw an increased expression of both of these proteins in ASH-treated animals compared with Veh-treated animals. However, we failed to detect any difference in the levels of TNF-α, a secreted cytokine from type M1 and M2b microglia (Fig. 4o).
Reduced p65 NF-κB Levels in the Spinal Cord of hTDP-43A315T Mice
Rescue of TDP-43 Mislocalization and Aggregation
Reduction of Peripherin Expression
The intermediate filament protein peripherin has been shown to be involved in neurite elongation at developmental stage and axonal regeneration but could also be responsible for protein aggregation and motor neuron death in ALS . In Veh-treated transgenic mice, peripherin was found to be prominently expressed. However, in the spinal cord of hTDP-43A315T mice treated with ASH, there was a marked reduction in peripherin levels (Fig. S2f). Note that peripherin levels in female hTDP-43A315T mice were higher than in male mice, irrespective of the treatment.
Nuclear TDP-43 Redistribution in Cell Culture System is Mediated by Secreted Factors From ASH-Treated Microglial Cells
We further investigated whether there was a direct effect of ASH on NSC34. On direct treatment of NSC34–hTDP-43A315T with 250 μg/ml ASH solution, almost 50% cell death was observed just after 4 h of incubation. By reducing the dose to 100 μg/ml no significant improvements were observed, but on further lowering the concentrations (10 and 1 μg/ml) the cells seemed to tolerate ASH (Fig. 7g). To test whether these low ASH concentrations could affect P65 NF-κB activity in NSC34–hTDP-43A315T cells, the P65 luc reporter was transfected, as described in the “Materials and Methods” section. The NSC34–hTDP-43A315T-P65 luc cells were then treated with low dosage of ASH, with or without stimulation with recombinant TNF-α. Luciferase assay from these cells clearly showed that these doses were sufficient to reduce significantly P65 activity post-TNF-α treatment (p < 0.01) (Fig. 7h). Interestingly, it was also observed that direct ASH treatment of glutamate-pretreated NSC34–TDP-43A315T cells did not alter TDP-43 distribution (Fig. 7i).
There is compelling evidence that the NF-κB signaling pathway may represent a rational therapeutic target for ALS. For instance, a number of ALS-linked genes encode proteins that may interact with the NF-κB signaling pathway: 1) TDP-43 and FUS can bind and activate p65 NF-κB [12, 30]; 2) ALS-linked mutations have been discovered in the optineurin gene, which encodes a protein activating the suppressor of NF-κB ; 3) mutations in valosin-containing protein activates NF-κB signalling [32, 33]; 4) overt inflammation was present in mice deficient for progranulin, a negative regulator of NF-κB activity ; 5) p62 (SQSTM1) can be associated with exacerbated inflammatory responses  and mutations in ALS/FTLD increase p62 levels ; 6) NF-kB signaling pathway is upregulated in ALS induced pluripotent stem cell-derived motor neurons ; 7) ablation of NF-κB signaling in microglia extended survival of superoxide dismutase 1 mutant mice .
To attenuate activation of the NF-κB signaling pathway in a transgenic mouse model of ALS, we tested an extract of W. somnifera (ASH) that had the potency to reduce in a dose-dependent fashion the activity of NF-κB P65–luciferase reporter in the microglial cultured BV2 cells (Fig. 1). The anti-inflammatory properties of ASH were further demonstrated by it role in inhibiting proinflammatory cytokines/chemokines released by primary mouse microglia as a result of an inflammatory stimulus. Among the variety of cytokines and chemokines affected, of particular interest are RANTES, IL-6, IL-17, and MIP-1α, whose circulating levels in serum and/or cerebrospinal fluid are reported to be elevated in ALS [39, 40, 41, 42]. Likewise, soluble TNF receptors R1 and R2 levels are also reported to be elevated in plasma of patients with ALS . IL-4 levels have been reported to be negatively correlated with IL-6 levels in cerebrospinal fluid of ALS cases. Thus, if IL-6 level increases, IL-4 decreases . In vitro, ASH restored LPS-induced downregulation of microglial IL-4 secretion and thus may contribute to modulation of inflammatory processes in multiple ways.
Transgenic mice expressing hTDP-43A315T genomic fragment used in this study exhibited age-associated pathologic changes, including TDP-43 proteinopathy, cognitive deficits, and motor dysfunction . When administered orally by gavage in hTDP-43A315T transgenic mice >1 year of age, ASH was found to ameliorate motor performance on rotarod test, as well as cognitive function, as determined by passive avoidance test. Moreover, ASH ameliorated muscle innervation, as well as pathological changes. Peripherin is an intermediate filament protein whose accumulation is a pathological hallmark of ALS and transgenic mouse studies demonstrated that sustained peripherin overexpression can cause motor neuron death [45, 46]. Peripherin levels are upregulated in the hTDP-43A315T transgenic mice , and ASH treatment caused a marked reduction in peripherin levels.
Of particular interest was the finding that ASH led to redistribution of cytoplasmic hTDP-43 to the nucleus in spinal motor neurons and in brain cortical neurons of TDP-43A315T transgenic mice after 8 weeks of treatment (Fig. 6a–d,f,g ). Moreover, ASH treatment reduced the levels of hTDP-43 recovered in the detergent-insoluble fraction of spinal cord (Fig. 6e). Thus, ASH treatment may confer protection by rescuing both the mislocalization and aggregation of hTDP-43. It is noteworthy that ASH treatment led to a significant reduction in levels of phospho-NF-κB in the nucleus of spinal motor neurons of hTDP-43A315T mice (Fig. 5a–c). This observation provides further evidence of a link between inflammation and TDP-43 pathology. This is in line with our recent report that chronic induction of inflammation by LPS treatment exacerbated TDP-43 proteinopathy in hTDP-43A315T mice .
The neuroprotective effects of ASH in this mouse model led us to further investigate whether ASH exerted benefits directly on neurons or indirectly on neurons via the action of the compound on other CNS cell types like glial cells. To address whether ASH may exert a direct protective effect on neurons, we used NSC34 motor neuron-like cells that were stably transfected to express hTDP-43A315T. Glutamate induced excitotoxic stress and cytoplasmic mislocalization of hTDP-43 in NSC34 cells. However, ASH treatment at10 μg/ml did not rescue this phenomenon (Fig. 7i), but treatment of glutamate-pretreated NSC34–hTDP-43A315T cells with conditioned media from ASH-treated microglial cells corrected the cytoplasmic mislocalization of hTDP-43. The factors from ASH-treated microglia involved in hTDP-43 redistribution into nucleus were inactivated by heat (Fig. 7f). Therefore, according to these in vitro results, the beneficial effects of ASH on neuronal pathology likely arise indirectly from the action of the drug on glial cells. Actually, it is well established that glial cells contribute to the pathogenesis of ALS . Astrocytes have been reported in multiple studies to be implicated in inducing motor neuron death [49, 50]. Yet, astrocyte activation in response to factors released from damaged motor neurons may also trigger the release of nerve growth factor and other antioxidative enzymes that confer neuroprotection . The role of microglia is also complex. In response to neurodegeneration or to accumulations of misfolded proteins, microglia can proliferate and adopt an activated state with secretion of several factors that may be either beneficial or detrimental to neurons [52, 53]. Hence, microglial responses can vary from neuroprotective (M2a state) to injurious/toxic states (M1, classically activated) . In our study, after 8 weeks of ASH treatment, we observed a significant reduction in GFAP expression in the spinal cord of hTDP-43A315T mice, as well as reduction of Iba-1 immunoreactivity in microglia, although many of these cells did not assume a quiescent morphology after ASH treatment. Immunoblotting of spinal cord extracts revealed an upregulation in levels of YM-1 and arginase-1, which are markers of microglial M2a phenotype.
In conclusion, our results demonstrated beneficial effects of ASH treatment on behavioral and pathologic phenotypes of transgenic mice expressing hTDP-43A315T. It remains to be determined what components in ASH are responsible for the therapeutic effects. One active molecule in ASH is Withaferin A, a steroid lactone known to act as NF-κB inhibitor and which has been shown to confer protection in 2 different mouse models of ALS [12, 26]. Nonetheless, other components present in ASH may also confer protection. For instance, sominone, a metabolite of withanoside IV has been shown to promote neurite outgrowth and to improve learning and memory deficits in mice . An extract of W. somnifera was also effective in reversing behavioral deficits and amyloid plaque burden in a mouse model of Alzheimer’s disease . In addition to diseases of the ALS/FTLD spectrum, TDP-43 pathology has been reported in other neurologic disorders including Alzheimer’s disease, Parkinson’s disease, Lewy body disease, cerebral ischemia, and hippocampal sclerosis . ASH is a natural health product, easily accessible, and has been well tolerated in previous studies. Nonetheless, there are some drawbacks to such a therapeutic approach. As with other plant extracts, it is still not clear which components of ASH are responsible for what specific beneficial effects and whether the benefits are due to compounds acting alone or synergistically with others in whole extracts. Moreover, huge variations in composition of ASH extracts may occur as a result of variations in the source of W. somnifera or methods of root extract preparations. Based on the findings presented here, it is of interest to further investigate whether ASH extract or it constituents might be effective in conferring therapeutic effects in TDP-43 proteinopathies like ALS and FTLD.
We thank Christine Bareil, Geneviève Soucy, and Pierre Cordeau Jr. for their technical assistance. We thank Valeant Pharmaceuticals International Inc (QC, Canada) for having kindly provided the Ashwagandha extract for our study. This work was funded by a grant from ALS Association (USA), the Canadian Institute of Health Research, and Hudson Team grant from ALS Society of Canada and Brain Canada Foundation. J.-P.J. holds a Canada Research Chair Tier 1 on mechanisms of neurodegeneration.
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