Endocan reduces the malign grade of gastric cancer cells by regulating associated protein expression
- 169 Downloads
Endocan, which has been identified to be low expressed in gastric cancer, was found to be positively related to the differentiation level of gastric cancer in vivo and in vitro. In the present study, we aimed to investigate the role of endocan in gastric adenocarcinoma cell line SGC7901 by artificially upregualting or downregulating endocan expression using endocan recombinant vector or specific small interfering RNA (siRNA)-targeting endocan gene, respectively. The effects of endocan recombinant vector-mediated over-expressing and siRNA-mediated endocan silencing on the differentiation, migration, and apoptosis of SGC7901 cells were assessed. Furthermore, the primary molecular mechanisms of endocan were explored by testing the expression alterations of associated protein in SGC7901 along endocan over-expression or knockdown. We found that over-expression of endocan reduced the migration but promoted the differentiation and apoptosis of SGC7901 cells. While, knockdown of endocan did just the opposite. Some molecules were found to participate in endocan-mediated anti-tumor effects, such as p53, caspase 3, and MMP-9. In conclusion, our findings suggest that endocan plays an anti-carcinogenic role in gastric cancer development and progression and might serve as a prognostic biomarker as well as a potential therapeutic target for gastric cancer.
KeywordsGastric cancer Endocan Biomarker
This study was supported by the Fund for Young Talents in the College of Anhui Province (No. 2012SQRL067), National Natural Science Foundation of China (No. 81201907), National Natural Science Foundation of China (No. 81272399), National Natural Science Foundation of China (No. 81470568), and Research Fund for Doctors in Anhui Medical University (XJ201229).
Compliance with ethical standards
Conflicts of interest
- 9.Grigoriu BD, Depontieu F, Scherpereel A, Gourcerol D, Devos P, Ouatas T, et al. Endocan expression and relationship with survival in human nonsmall cell lung cancer. Clin cancer res off J Am Assoc. Cancer Res. 2006;12:4575–82.Google Scholar
- 12.Rennel E, Mellberg S, Dimberg A, Petersson L, Botling J, Ameur A, et al. Endocan is a VEGF-A and PI3K regulated gene with increased expression in human renal cancer. Exp Cell Res 2007; 313: 1285–1294.Google Scholar
- 13.Xu Z, Zhang SM, Zhou Q, Wang Y, Xia RX. Endocan, a potential prognostic and diagnostic biomarker of acute leukemia. Mol Cell Biochem 2014; 395: 117–123.Google Scholar
- 20.Bao L, Liu F, Guo HB, Li Y, Tan BB, Zhang WX, et al. Naringenin inhibits proliferation, migration, and invasion as well as induces apoptosis of gastric cancer SGC7901 cell line by downregulation of AKT pathway. Tumour Biol. 2016; [Epub ahead of print].Google Scholar
- 22.Fields GB. New strategies for targeting matrix metalloproteinases. Matrix Biol 2015; 44–46:239–246.Google Scholar
- 23.Major apoptotic mechanisms and genes involved in apoptosis. Kiraz Y, Adan A, Kartal Yandim M, Baran Y. Tumour Biol. 2016 Apr 9.Google Scholar