Tumor Biology

, Volume 37, Issue 10, pp 13499–13508 | Cite as

Clinicopathological and prognostic significance of Yes-associated protein expression in hepatocellular carcinoma and hepatic cholangiocarcinoma

  • Hao Wu
  • Yan Liu
  • Xiao-Wei Jiang
  • Wen-Fang Li
  • Gang Guo
  • Jian-Ping Gong
  • Xiong DingEmail author
Original Article


Hepatocellular carcinoma (HCC) and hepatic cholangiocarcinoma (CC) are the most aggressive malignancies with a poor prognosis in humans, and hepatic cholangiocarcinoma (CC) exhibits greater malignant behaviour. Yes-associated protein (YAP) is an important downstream target of the Hippo signalling pathway. As an oncogene, it plays a vital role in the occurrence and development of tumours. Our study focuses on the clinical significance of YAP protein expression in HCC and CC. Furthermore, we sought to explore the different survival rates between HCC and CC. A total of 137 patients with HCC and 122 with CC after resection were evaluated by immunohistochemistry for the expression of YAP. Our results showed that positive expression rates of YAP were more frequently noted in CC 67.2 % (82/122) than in HCC 56.9 % (78/137) (P = 0.024). High YAP expression in HCC and CC was significantly associated with tumour size (P < 0.001 and P = 0.019, respectively), liver cirrhosis (P = 0.002 and P = 0.009, respectively), vascular invasion (P = 0.047 and P = 0.018, respectively), multiplicity (P = 0.019 and P = 0.015, respectively), and intrahepatic metastasis (P = 0.015 and P = 0.047, respectively). Importantly, recurrence-free survival and disease-specific survival rates were lower in CC with high YAP expression than in HCC with high YAP expression (P < 0.001 and P < 0.001, respectively). Overall, high YAP expression was more frequently found in CC than in HCC, and YAP overexpression was associated with poor survival rates in patients with HCC and CC. Targeting YAP treatment requires further prospective investigations in larger patient populations.


Hepatocellular carcinoma Hepatic cholangiocarcinoma Yes-associated protein(YAP) Immunohistochemistry 



This research was funded by the National Natural Science Foundation of China, projects No 81272570 and the Health Bureau of Chongqing City, projects No 2015ZDXM012.

Compliance with ethical standards

Conflicts of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2016

Authors and Affiliations

  • Hao Wu
    • 1
  • Yan Liu
    • 2
  • Xiao-Wei Jiang
    • 3
  • Wen-Fang Li
    • 4
  • Gang Guo
    • 5
  • Jian-Ping Gong
    • 1
  • Xiong Ding
    • 1
    Email author
  1. 1.Department of Hepatobiliary SurgeryThe Second Affiliated Hospital of Chongqing Medical UniversityChongqingChina
  2. 2.Department of GastroenterologyThe Fifth people’s Hospital of ChengduChengduChina
  3. 3.Department of General SurgeryThe Fifth people’s Hospital of ChongqingChongqingChina
  4. 4.Department of General SurgeryThe Taihe Hospital Affiliated to Hubei Medical UniversityShiyanChina
  5. 5.Laboratory of PathologyWest China Hospital of Sichuan UniversityChengduChina

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