Tumor Biology

, Volume 37, Issue 9, pp 11815–11824 | Cite as

High miR-96 levels in colorectal adenocarcinoma predict poor prognosis, particularly in patients without distant metastasis at the time of initial diagnosis

  • Stamatia-Maria Rapti
  • Christos K. Kontos
  • Iordanis N. Papadopoulos
  • Andreas Scorilas
Original Article


MicroRNA-96 (miR-96) is an oncomiR that facilitates the development of malignant tumors by promoting growth, proliferation, and survival of cancer cells. Previous studies using high-throughput techniques have shown that miR-96 is upregulated in colorectal cancer compared to adjacent normal colorectal tissue. The aim of this study was the investigation of the potential clinical value of miR-96 as a molecular prognostic biomarker in colorectal adenocarcinoma. For this purpose, total RNA was extracted from 108 primary colorectal adenocarcinoma samples and 54 paired non-cancerous colorectal tissue specimens. After polyadenylation and reverse transcription, miR-96 molecules were determined using an in-house developed real-time quantitative PCR based on SYBR Green chemistry. Calculations were carried out with the comparative CT method, using SNORD48 as endogenous reference gene. Finally, extensive biostatistical analysis was performed and showed that miR-96 is significantly upregulated in colorectal adenocarcinoma specimens compared to their non-cancerous counterparts (p < 0.001) as well as in tumors having invaded regional lymph nodes (p = 0.009) and those of advanced TNM stage (p = 0.008). miR-96 expression is an unfavorable prognostic marker in colorectal adenocarcinoma, predicting poor disease-free and overall survival (p = 0.041 and 0.028, respectively), independently of classical clinicopathological parameters. Most importantly, miR-96 expression stratifies patients without distant metastasis (M0) at the time of diagnosis into two groups with substantially different prognosis (p = 0.040). In conclusion, high tissue levels of miR-96 are associated with advanced stages of colorectal adenocarcinoma and predict an increased risk for disease recurrence and poor overall survival, especially in patients without distant metastasis at the time of diagnosis.


MicroRNAs (miRNAs) Molecular tumor markers Prognostic biomarkers Colorectal cancer Real-time quantitative PCR (qPCR) 


Compliance with ethical standards

The study was approved by the institutional Ethics Committee of the University General Hospital “Attikon” in accordance with the ethical standards of the Declaration of Helsinki and informed consent was obtained from the patients.

Conflicts of interest

The authors declare that they have no conflict of interest.

Supplementary material

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High resolution image (TIF 562 kb)
13277_2016_5023_MOESM2_ESM.doc (84 kb)
ESM 2 (DOC 84 kb)


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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2016

Authors and Affiliations

  • Stamatia-Maria Rapti
    • 1
  • Christos K. Kontos
    • 1
  • Iordanis N. Papadopoulos
    • 2
  • Andreas Scorilas
    • 1
  1. 1.Department of Biochemistry and Molecular BiologyUniversity of AthensAthensGreece
  2. 2.Fourth Surgery DepartmentUniversity General Hospital “Attikon”AthensGreece

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