Tumor Biology

, Volume 37, Issue 8, pp 10487–10497 | Cite as

DNA methyltransferase 1, 3a, and 3b expression in hepatitis C associated human hepatocellular carcinoma and their clinicopathological association

  • Nadir Naveed Siddiqui
  • Ahtesham ul Haq
  • Owais Ali Siddiqui
  • Rizma Khan
Original Article


Identification of biomarker will obligate a substantial influence on various cancer management and treatment. We hypothesize that genetic/proteomic and epigenetic studies should be uncovering modifications which may be independently or jointly affect the expression of the genes that are involved in the progression of liver cancer (LC). For this purpose, we examined the effect of expressional changes of DNMTs on HCV infected LC of different genotypes. We found that both mRNA and protein expression levels of DNMT1, 3a, and 3b were upregulated in genotype 1b and 3a HCV infected patients as compared to control. However, DNMT3b mRNA levels did not change in genotypes 2a, 3, and 4, but were upregulated at the protein level by genotype 1b, 2a, and 3a. Furthermore, no significant changes were observed for DNMTs investigated in sample expressing the genotypes 5 and 6. Our findings suggest that HCV at least in part by altering DNMTs expression may play a significant role in HCC progression.


Hepatocellular carcinoma DNA methyltransferase 1 3a and 3b Two dimensional gel electrophoresis Western blotting Real time PCR 



We graciously thank Dr. N. Kabir (Panjwani Center, ICCBS, and University of Karachi-Pakistan) for providing the facility of immunohistochemistry.

The authors also thank Dr. Abid Ali (HEJ, University of Karachi) and Kamran Syed (Chemical House) for providing 2DE facility. A part of this study was performed at the Industrial Biotechnology Department of The Karachi Institute of Biotechnology and Genetic Engineering, (KIBGE), University of Karachi, Karachi, Pakistan.

Compliance with ethical standards

Conflicts of interest


Supplementary material

13277_2016_4941_MOESM1_ESM.docx (19 kb)
ESM 1 (DOCX 18 kb)


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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2016

Authors and Affiliations

  • Nadir Naveed Siddiqui
    • 1
  • Ahtesham ul Haq
    • 2
    • 3
  • Owais Ali Siddiqui
    • 3
  • Rizma Khan
    • 3
    • 4
  1. 1.The Karachi Institute of Biotechnology and Genetic Engineering, (KIBGE)University of KarachiKarachiPakistan
  2. 2.Department of BiochemistryUniversity of Karachi-PakistanKarachiPakistan
  3. 3.Department of Molecular GeneticsDr. Ziauddin HospitalKarachiPakistan
  4. 4.Department of BiochemistryZiauddin UniversityKarachiPakistan

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