Biological characteristics of a novel giant cell tumor cell line derived from spine
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Giant cell tumor of bone(GCTB) is a special bone tumor for it consists of various cell types, and its biological characteristics is different from common benign or malignant neoplasm. In the present study, we report the biological features of a primary Asian GCTB cell line named GCTB28. We analyzed extensive properties of the GCTB28 cells including morphological observations, growth, cell cycle, karyotype, proliferation, proteins expression, surface biomarker verification, and tumorigenicity in nude mice. We found that the stromal cells of GCTB were endowed with self-renewal capacity and played dominant roles in GCTB development. Moreover, we confirmed that GCTB cells can be CD33−CD14− phenotype which was not in accord with previous study. This study provides an in vitro model system to investigate pathogenic mechanisms and molecular characteristics of GCTB and also provides a useful tool for researching the therapeutic targeting of GCTB.
KeywordsGiant cell tumor of bone (GCTB) Biomarker Mononuclear stromal cells Multinuclear giant cells Novel cell line of GCTB
Compliance with ethical standards
This study was funded by grants from the Biobank Project of Shanghai (12DZ2295103), Bone Tumor Sample Databases and Digital Information Platform Project of Shanghai (08DZ2292800), and Shanghai Biobank Network of Common Human Tumor Tissue (12DZ2295100).
Conflicts of interest
Animals were involved in the present study. All experimental operations on animals were approved by the Animal Ethics Committees of the Second Military Medical University.
Human tissues were involved in the present study. All procedures performed in studies involving human participants were in accordance with the ethical standards of the Clinical Research Ethics Committee of Second Military Medical University and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. Tumor tissues were obtained with informed consent, and this study was approved by the Clinical Research Ethics Committee of Second Military Medical University.
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