Tumor Biology

, Volume 37, Issue 6, pp 7481–7491 | Cite as

Tumor-suppressive microRNA-34a inhibits breast cancer cell migration and invasion via targeting oncogenic TPD52

Original Article

Abstract

The tumor protein D52 (TPD52) is an oncogene overexpressed in breast cancer. Although the oncogenic effects of TPD52 are well recognized, how its expression and the role in migration/invasion is still not clear. This study tried to explore the regulative role of microRNA-34a (miR-34a), a tumor suppressive miRNA, on TPD52 expression in breast cancer. The expression of miR-34a was found significantly decreased in breast cancer specimens with lymph node metastases and breast cancer cell lines. The clinicopathological characteristics analyzed showed that lower expression levels of miR-34a were associated with advanced clinical stages. Moreover, TPD52 was demonstrated as one of miR-34a direct targets in human breast cancer cells. miR-34a was further found significantly repress epithelial-mesenchymal transition (EMT) and inhibit breast cancer cell migration and invasion via TPD52. These findings indicate that miR-34a inhibits breast cancer progression and metastasis through targeting TPD52. Consequently, our data strongly suggested that oncogenic TPD52 pathway regulated by miR-34a might be useful to reveal new therapeutic targets for breast cancer.

Keywords

Human breast cancer miR-34a TPD52 Epithelial-mesenchymal transition 

Abbreviations

3′-UTR

3′-Untranslated region

DMEM

Dulbelcco’s modified Eagle’s media

EMT

Epithelial-mesenchymal transition

FBS

Fetal bovine serum

IHC

Immunohistochemistry

IRB

Institutional Review Board

KRT19

Keratin 19

miRNA

MicroRNA

PBS

Phosphate-buffered saline

qRT-PCR

Quantitative real-time reverse transcription-PCR

TGF-β

Transforming growth factor-beta

TPD52

Tumor protein D52

Notes

Acknowledgments

This study was supported by the Foundation of Educational Committee of Heilongjiang Province of China (Grant No. 12541311).

Compliance with ethical standards

The authors declare that all the patients, involved in this study, signed informed consent forms according to our institutional guidelines, and the study was approved by Institutional Review Board (IRB) protocols of Harbin Medical University. All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Conflicts of interest

None

Supplementary material

13277_2015_4623_Fig6_ESM.gif (71 kb)
Supplementary Figure 1

Quantitive analysis for western blot results of TPD52 expression treated with miR-34a mimic or inhibitor in MDA-MB-231 and MCF-7 cells. Western blot analysis of TPD52 expression treated with miR-34a mimic or inhibitor was performed triplicate in MDA-MB-231 and MCF-7 cells. β-actin was used as an internal control. The band density was determined using the ImageJ software for quantitative analysis (*P<0.05 compared with control group). (GIF 71 kb)

13277_2015_4623_MOESM1_ESM.tif (815 kb)
High Resolution (TIF 815 kb)
13277_2015_4623_MOESM2_ESM.doc (46 kb)
Supplementary Table 1(DOC 46 kb)
13277_2015_4623_MOESM3_ESM.doc (39 kb)
Supplementary Table 2(DOC 39 kb)

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Guodong Li
    • 1
    • 2
  • Lei Yao
    • 3
  • Jinning Zhang
    • 1
    • 2
  • Xinglong Li
    • 1
    • 2
  • Shuwei Dang
    • 1
    • 2
  • Kai Zeng
    • 1
    • 2
  • Yuhui Zhou
    • 1
    • 2
  • Feng Gao
    • 3
  1. 1.Department of General Surgerythe Fourth Affiliated Hospital of Harbin Medical UniversityHarbinChina
  2. 2.Bio-Bank of Department of General Surgerythe Fourth Affiliated Hospital of Harbin Medical UniversityHarbinChina
  3. 3.Department of General Surgerythe Second Affiliated Hospital of Harbin Medical UniversityHarbinChina

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