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Tumor Biology

, Volume 37, Issue 4, pp 5591–5597 | Cite as

BAG3 regulates cell proliferation, migration, and invasion in human colorectal cancer

  • Huiyong Shi
  • Haidong Xu
  • Zengjun Li
  • Yanan Zhen
  • Bin Wang
  • Shoujun Huo
  • Ruixue Xiao
  • Zhongfa XuEmail author
Original Article

Abstract

Bcl2-associated athanogene 3 (BAG3) has been reported to be elevated in various tumors. However, it is unclear whether BAG3 has a functional role in the initiation and progression of colorectal cancer (CRC). Here, we collected CRC samples and cell lines to validate the pathway by using gene and protein assays. RT-PCR showed that the expression of BAG3 mRNA in CRC tissues was obviously higher than that in non-tumor tissues (p < 0.001). Immunohistochemical analysis showed that immunoreactivity of BAG3 was found in most CRC tissues and strongly correlated with TNM stage (p = 0.001), differentiation (p = 0.003), and metastasis (p = 0.010). Low expression of BAG3 in HCT-8 significantly reduced cellular proliferation, migration, and invasion. The analysis of in vitro cell showed that HCT-8 cells were exposed to si-BAG3, and its growth was inhibited depending on modulation of cell cycle G1/S checkpoints and cell cycle regulators, involving cyclin D1, cyclin A2, and cyclin B1. Furthermore, suppression of the epithelial-mesenchymal transition (EMT) by si-BAG3 is linked to the decreased expression of E-cadherin and the increased expression of N-cadherin, vimentin, and MMP9. In conclusion, in the present study, we demonstrated that BAG3 overexpression plays a critical role in cell proliferation, migration, and invasion of colorectal cancer. Our data suggests targeted inhibition of BAG3 may be useful for patients with CRC.

Keywords

BAG3 EMT CRC 

Notes

Acknowledgments

We greatly thank other members of our lab for valuable suggestions and writing.

Compliance with ethical standards

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Huiyong Shi
    • 1
  • Haidong Xu
    • 1
  • Zengjun Li
    • 2
  • Yanan Zhen
    • 1
  • Bin Wang
    • 1
  • Shoujun Huo
    • 1
  • Ruixue Xiao
    • 1
  • Zhongfa Xu
    • 1
    Email author
  1. 1.Department of General SurgeryAffiliated Hospital of Shandong Academy of Medical SciencesJinanChina
  2. 2.Department of General SurgeryShandong Cancer Hospital and InstituteJinanChina

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