Tumor Biology

, Volume 37, Issue 4, pp 4649–4654 | Cite as

RETRACTED ARTICLE: Overexpression of OCT4 contributes to progression of hepatocellular carcinoma

  • Gang Xu
  • Fuzhen Qi
  • Jianhuai Zhang
  • Jianbo Xu
  • Ting Shi
  • Yi Miao
Original Article
  • 906 Downloads

Abstract

The abnormal change of octamer transcription factor 4 (OCT4) is associated with tumor progression; however, its effect on hepatocellular carcinoma (HCC) behavior remains unclear. The purpose of this study was to determine the correlation between HCC and OCT4. In the present study, IHC, western blot analysis, and QRT-PCR were performed to identify differentially expressed OCT4 in a series of HCC tissues and adjacent non-cancerous tissues. In addition, the functions of OCT4 on HCC progression were studied in vitro. Silencing of OCT4 with siRNA was performed in HCC cell lines, and the impact on proliferation, migration, and the EMT marker of HCC was analyzed. Our results found that OCT4 levels were significantly higher in HCC tissues compared with the adjacent non-cancerous tissues. Furthermore, OCT4 siRNA significantly reduced the proliferation rate of SMMC-7721 and HepG2 cells, inhibited the migration and inversion, and could reverse EMT in HCC cells, indicating that OCT4 plays a critical role in HCC progression. Our data suggest that the pathogenesis of human HCC may be mediated by OCT4, and thus, OCT4 could represent selective targets for the molecularly targeted treatments of HCC.

Keywords

Hepatocellular carcinoma Octamer transcription factor 4 Epithelial-to-mesenchymal transition Proliferation Migration 

Notes

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Gang Xu
    • 1
  • Fuzhen Qi
    • 1
  • Jianhuai Zhang
    • 1
  • Jianbo Xu
    • 1
  • Ting Shi
    • 1
  • Yi Miao
    • 2
  1. 1.Department of General SurgeryHuai’an First People’s Hospital, Nanjing Medical UniversityHuai’anChina
  2. 2.Department of General SurgeryFirst Affiliated Hospital of Nanjing Medical UniversityNanjingChina

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