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Tumor Biology

, Volume 37, Issue 3, pp 3599–3607 | Cite as

Recurrence of squamous cell lung carcinoma is associated with the co-presence of reactive lesions in tumor-adjacent bronchial epithelium

  • Olga V. Pankova
  • Evgeny V. DenisovEmail author
  • Anastasia A. Ponomaryova
  • Tatiana S. Gerashchenko
  • Sergey A. Tuzikov
  • Vladimir M. Perelmuter
Original Article

Abstract

Recurrences occur in 30 % of lung cancer patients after radical therapy; however, known prognostic factors are not always effective. In this study, we investigated whether the frequency of squamous non-small cell lung cancer (NSCLC) recurrence depends on the presence of reactive lesions in tumor-adjacent bronchial epithelium. Specimens of adjacent lung tissue from 104 patients with squamous NSCLC were used for the determination of basal cell hyperplasia (BCH) and squamous metaplasia (SM) and for the analysis of the expression of Ki-67, p53, Bcl-2, and CD138. We found that recurrence was observed in 36.7 % of patients with BCH combined with SM (BCH + SM+) in the same bronchus, compared with 1.8 % in patients with isolated BCH (BCH + SM−; odds ratio (OR) 31.26, 95 % confidence interval (CI) 3.77–258.60; p = 0.00002). The percentage of Ki-67-positive cells was significantly higher in BCH + SM+ than in BCH + SM− (34.9 vs. 18.3 %; effect size 2.86, 95 % CI 2.23–3.47; p = 0.003). P53 expression was also more significant in BCH + SM+ than in BCH + SM− (14.4 vs. 9.6 %; effect size 1.22, 95 % CI 0.69–1.76; p = 0.0008). In contrast, CD138 expression was lower in BCH + SM+ than in BCH + SM− (21.8 vs. 38.5 %; effect size −6.26, 95 % CI −7.31 to −5.22; p = 0.003). Based on our results, we concluded that the co-presence of reactive bronchial lesions is associated with the development of recurrent squamous NSCLC and may be a negative prognostic indicator. In addition, significant differences in Ki-67, p53, and CD138 expression exist between isolated BCH and BCH combined with SM that probably reflect part of biological differences, which could relate to the mechanism of lung cancer recurrence.

Keywords

Lung cancer Recurrence Hyperplasia Metaplasia Immunohistochemistry 

Notes

Acknowledgments

The study was supported by the Russian Scientific Foundation (project #14-15-00350). E.V. Denisov was supported by Tomsk State University Competitiveness Improvement Program.

Conflicts of interest

None

Supplementary material

13277_2015_4196_MOESM1_ESM.pdf (192 kb)
ESM 1 (PDF 192 kb)

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Olga V. Pankova
    • 1
  • Evgeny V. Denisov
    • 2
    • 3
    • 4
    Email author
  • Anastasia A. Ponomaryova
    • 2
    • 5
  • Tatiana S. Gerashchenko
    • 2
    • 3
  • Sergey A. Tuzikov
    • 6
  • Vladimir M. Perelmuter
    • 1
    • 7
  1. 1.Department of Pathological Anatomy and CytologyTomsk Cancer Research InstituteTomskRussia
  2. 2.Laboratory of Molecular Oncology and ImmunologyTomsk Cancer Research InstituteTomskRussia
  3. 3.Laboratory for Translational Cellular and Molecular BiomedicineNational Research Tomsk State UniversityTomskRussia
  4. 4.Department of Organic ChemistryNational Research Tomsk State UniversityTomskRussia
  5. 5.National Research Tomsk Polytechnic UniversityTomskRussia
  6. 6.Department of Thoracoabdominal OncologyTomsk Cancer Research InstituteTomskRussia
  7. 7.Department of Pathological AnatomySiberian State Medical UniversityTomskRussia

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