Skip to main content

Advertisement

SpringerLink
Log in

Epigenetic downregulated ITGBL1 promotes non-small cell lung cancer cell invasion through Wnt/PCP signaling

  • Original Article
  • Published:
Tumor Biology

An Erratum to this article was published on 29 February 2016

Abstract

Integrin, beta-like 1 (ITGBL1), is a β-integrin-related extracellular matrix protein which contains ten EGF-like repeats domain. Surprisingly, we screen Oncomine Database and found that ITGBL1 is more commonly downregulated in non-small cell lung cancer (NSCLC) tissues, and the result reminds us to explore its significance in NSCLC. Thus, we retrieved DRUGSURV Database and found that downregulated ITGBL1 predicts a poor prognosis of patients. These results provided us the clues that ITGBL1 might be a tumor suppressor in NSCLC. However, the biological functions of ITGBL1 have not been reported to date. In the current study, we surprisingly found that knockdown of ITGBL1 in NSCLC cell lines could promote cancer cell migration and invasion. Furthermore, recombinant ITGBL1 protein-treated cancer cell could inhibit cell migration and invasion. These results suggested that ITGBL1 plays a suppressive role in NSCLC progression. We further found that the downregulation of ITGBL1 might result from highly expressed miR-576-5p in NSCLC tissues, and the activity of Wnt/PCP signaling was enhanced when the level of ITGBL1 was inhibited. In conclusion, our results suggest that ITGBL1 is a novel tumor suppressor in NSCLC progression.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Herbst RS, Heymach JV, Lippman SM. Lung cancer. The New England journal of medicine. 2008;359:1367–80.

    Article  CAS  PubMed  Google Scholar 

  2. Siegel R, Naishadham D, Jemal A. Cancer statistics, 2013. CA Cancer J Clinsss. 2013;63:11–30.

    Article  Google Scholar 

  3. Molina JR, Yang P, Cassivi SD, Schild SE, Adjei AA. Non-small cell lung cancer: epidemiology, risk factors, treatment, and survivorship. Mayo Clin Proc. 2008;83(5):584–94.

    Article  PubMed  PubMed Central  Google Scholar 

  4. Little AG, Gay EG, Gaspar LE, Stewart AK. National survey of non-small cell lung cancer in the United States: epidemiology, pathology and patterns of care. Lung Cancer. 2007;57:253–60.

    Article  PubMed  Google Scholar 

  5. Lu P, Weaver VM, Werb Z. The extracellular matrix: a dynamic niche in cancer progression. J Cell Biolss. 2012;196:395–406.

    Article  CAS  Google Scholar 

  6. Tsunoda T, Furusato B, Takashima Y, Ravulapalli S, Dobi A, Srivastava S, et al. The increased expression of periostin during early stages of prostate cancer and advanced stages of cancer stroma. Prostate. 2009;69:1398–403.

    Article  CAS  PubMed  Google Scholar 

  7. Tilman G, Mattiussi M, Brasseur F, van Baren N, Decottignies A. Human periostin gene expression in normal tissues, tumors and melanoma: Evidences for periostin production by both stromal and melanoma cells. Mol Cancer. 2007;6:80.

    Article  PubMed  PubMed Central  Google Scholar 

  8. Morra L, Moch H. Periostin expression and epithelial-mesenchymal transition in cancer: a review and an update. Virchows Archiv. 2011;459:465–75.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Fu Y, Feng M-X, Yu J, Ma M-ZM, Liu X-J, Li J, et al. DNA methylation-mediated silencing of matricellular protein dermatopontin promotes hepatocellular carcinoma metastasis by α3β1 integrin-rho gtpase signaling. Oncotarget. 2014;5:6701–15.

    Article  PubMed  PubMed Central  Google Scholar 

  10. Berg RW, Leung E, Gough S, Morris C, Yao WP, Wang SX, et al. Cloning and characterization of a novel beta integrin-related cdna coding for the protein tied ("ten beta integrin egf-like repeat domains") that maps to chromosome band 13q33: a divergent stand-alone integrin stalk structure. Genomics. 1999;56:169–78.

    Article  CAS  PubMed  Google Scholar 

  11. Esposito V, Baldi A, De Luca A, Groger AM, Loda M, Giordano GG, et al. Prognostic role of the cyclin-dependent kinase inhibitor p27 in non-small cell lung cancer. Cancer Res. 1997;57:3381–5.

    CAS  PubMed  Google Scholar 

  12. Katoh M. Wnt/PCP signaling pathway and human cancer (review). Oncol Rep. 2005;14:1583–8.

    CAS  PubMed  Google Scholar 

  13. Das PM, Singal R. DNA methylation and cancer. J Clin Oncol. 2004;22:4632–42.

    Article  CAS  PubMed  Google Scholar 

  14. Kouwenhove M, Kedde M, Agami R. MicroRNA regulation by RNA-binding proteins and its implications for cancer. Nat Rev Cancer. 2011;11:644–56.

    Article  PubMed  Google Scholar 

  15. Sawan C, Herceg Z. Histone modifications and cancer. Adv Genet. 2010;70:57–85.

    CAS  PubMed  Google Scholar 

  16. Kanwal R, Gupta S. Epigenetic modifications in cancer. Clin Genet. 2012;81:303–11.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Respiratory Medicine, The First Affiliated Hospital of Nanchang University, Nanchang, China

    Bo Tong

  2. Jiangxi Provincial People’s Hospital, No. 152, Aiguo Road, Nanchang, 330006, China

    Xin Gan

  3. Department of Tumor Chemotherapy, Jiangxi Tumor Hospital, Nangchang, 330029, China

    Zhentian Liu

  4. Department of Internal medicine, Jiangxi Tumor Hospital, Nangchang, 330029, China

    Jianying Zhou

Authors
  1. Xin Gan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zhentian Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Bo Tong
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jianying Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Bo Tong.

Additional information

Xin Gan and Zhentian Liu contributed equally to this work.

An erratum to this article is available at http://dx.doi.org/10.1007/s13277-016-4834-3.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Gan, X., Liu, Z., Tong, B. et al. Epigenetic downregulated ITGBL1 promotes non-small cell lung cancer cell invasion through Wnt/PCP signaling. Tumor Biol. 37, 1663–1669 (2016). https://doi.org/10.1007/s13277-015-3919-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13277-015-3919-8

Keywords

Search

Navigation