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Tumor Biology

, Volume 36, Issue 12, pp 9189–9199 | Cite as

RETRACTED ARTICLE: High expression of ErbB3 binding protein 1 (EBP1) predicts poor prognosis of pancreatic ductal adenocarcinoma (PDAC)

  • Chen Gong
  • Yixin Zhang
  • Yinji Chen
  • Haifeng Zhang
  • Xiaorong Liu
  • Huaqing Xue
  • Li Ji
  • Liang Wang
  • Linlin Yang
  • Guoxiong Zhou
  • Chunhua Wan
Research Article
  • 879 Downloads

Abstract

Recent studies have identified that ErbB3 binding protein 1 (EBP1) is broadly expressed in various cancer tissues and critically involved in plenty of biological processes in this regard. However, the functional role of EBP1 in pancreatic ductal adenocarcinoma (PDAC) has never been elucidated. In this study, we found that EBP1 could serve as a prognostic biomarker of PDAC. Western blot analysis revealed that EBP1 was remarkably upregulated in PDAC tissues and cell lines. Using immunohistochemical analysis, we showed that the expression of EBP1 was correlated with tumor size (P = 0.004), histological differentiation (P = 0.041), and tumor node metastasis (TNM) stage (P = 0.000). Notably, Kaplan–Meier curve showed that high expression of EBP1 predicted significantly worsened prognosis of PDAC patients (P = 0.001). In addition, knockdown of EBP1 expression suppressed PDAC cell proliferation and retarded cell cycle progression. Furthermore, depletion of EBP1 induced the apoptosis of Panc-1 cells. Of great interest, we found that EBP1 interacted with anti-apoptotic protein, Bcl-xL, and promoted its accumulation. In summary, our results suggest that EBP1 is a novel prognostic indicator and potential therapeutic target of PDAC, shedding new insights into the important role of EBP1 in cancer development.

Keywords

EBP1 Pancreatic ductal adenocarcinoma (PDAC) Prognosis Proliferation Apoptosis 

Notes

Acknowledgments

This work was supported by the National Natural Scientific Foundation of China (No. 81072028) and Jiangsu Province’s Outstanding Medical Academic Leader program (No. LJ101135).

Conflicts of interest

None

Supplementary material

13277_2015_3625_Fig7_ESM.gif (16 kb)
Supplemental Fig. 1

Graphic representation of the relationship between EBP1 and Ki-67 expression in 191 PDAC specimens. The scatter plots of EBP1 versus Ki-67 with regression line showing a correlation of them using the Spearman’s correlation coefficient. (GIF 15 kb)

13277_2015_3625_MOESM1_ESM.tif (171 kb)
(TIFF 171 kb)

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Chen Gong
    • 1
    • 2
  • Yixin Zhang
    • 3
  • Yinji Chen
    • 4
  • Haifeng Zhang
    • 1
  • Xiaorong Liu
    • 2
  • Huaqing Xue
    • 2
  • Li Ji
    • 1
  • Liang Wang
    • 1
  • Linlin Yang
    • 2
  • Guoxiong Zhou
    • 1
  • Chunhua Wan
    • 2
  1. 1.Department of GastroenterologyAffiliated Hospital of Nantong UniversityNantongChina
  2. 2.Jiangsu Province Key Laboratory for Inflammation and Molecular Drug TargetNantong UniversityNantongChina
  3. 3.Department of General SurgeryNantong University Cancer HospitalNantongChina
  4. 4.National Engineering Lab of Food Storage and TransportationNanjing University of Finance and EconomicsNanjingChina

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