Tumor Biology

, Volume 36, Issue 12, pp 9223–9231 | Cite as

Elevated expression of TGIF is involved in lung carcinogenesis

  • Yadong WangEmail author
  • Haiyu Wang
  • Huiyan Gao
  • Bing Xu
  • Wenlong Zhai
  • Jiangmin Li
  • Congke Zhang
Research Article


The purpose of this study was to explore the expression of TG-interacting factor (TGIF) in lung carcinogenesis. Malignant transformation of human bronchial epithelial (16HBE) cell was established by benzo(a)pyrene (BaP) treatment. Soft agar assay and tumor formation assay in nude mice were applied. Tumorigenesis experiment in vivo was done by BaP treatment. Western blotting, immunohistochemistry, and quantitative polymerase chain reaction were used to detect TGIF expression. We observed a higher level of TGIF messenger RNA (mRNA) in lung cancer tissues than that in paracancerous tissues. We observed significantly higher levels of TGIF mRNA and protein in A549 and H1299 cell lines than that in 16HBE cell. Increased expressions of TGIF protein and mRNA were observed in 16HBE cells induced by BaP treatment as compared to those in solvent control group. We observed significantly higher levels of TGIF mRNA and protein in 16HBE-BaP cells than that in 16HBE-control cells. We observed significantly higher levels of TGIF mRNA and protein in mice lung tissues treated with BaP than that in control group. Our results suggested that elevated expression of TGIF was involved in lung carcinogenesis.


TGIF Lung carcinogenesis Benzo(a)pyrene Lung cancer 



This work was supported by the National Natural Science Foundation of China (U1404815).

Conflicts of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Yadong Wang
    • 1
    Email author
  • Haiyu Wang
    • 1
  • Huiyan Gao
    • 1
  • Bing Xu
    • 1
  • Wenlong Zhai
    • 2
  • Jiangmin Li
    • 1
  • Congke Zhang
    • 1
  1. 1.Department of ToxicologyHenan Center for Disease Control and PreventionZhengzhouChina
  2. 2.Department of General SurgeryFirst Affiliated Hospital of Zhengzhou UniversityZhengzhouChina

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